Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana

Shahnawaz,; Pandey, Devendra Kumar; Konjengbam, Merinashwari; Dwivedi, Padmanabh; Kaur, Prabhjot; Kumar, Vijay; Ray, Durga; Rây, Prafulla Chandra; Nazir, Romaan; Kaur, Harmeet; Parida, Sidharth; Dey, Abhijit

doi:10.1007/s00253-021-11580-9

Cited by 16 publications

(2 citation statements)

References 135 publications

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“…[103] 2.4 Application of CRISPR/Cas system in natural product isolation from higher plants Medicinal plants are considered as valuable source of secondary metabolites used as biomedicine and in pharmacotherapy. [42,[104][105][106][107] Most studies of CRISPR/Cas applications in plants have been pre-liminary studies to prove the concept. Plant genome editing was achieved by using a human codon-optimized or a plant codonoptimized CRISPR/Cas9 enzyme with an NLS in the NHEJ-mediated repair of DNA DSBs.…”

Section: Spcas9mentioning

confidence: 99%

Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges

Biswas

Anand

Ghorai

et al. 2022

Biotechnology Journal

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An incredible array of natural products is produced by plants that serve several ecological functions, including protecting them from herbivores and microbes, attracting pollinators, and dispersing seeds. In addition to their obvious medical applications, natural products serve as flavoring agents, fragrances, and many other uses by humans.With the increasing demand for natural products and the development of various gene engineering systems, researchers are trying to modify the plant genome to increase the biosynthetic pathway of the compound of interest or blocking the pathway of unwanted compound synthesis. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has had widespread success in genome editing due to the system's high efficiency, ease of use, and accuracy which revolutionized the genome editing system in living organisms. This study highlights the method of the CRISPR/Cas system, its application in different organisms including microbes, algae, fungi, and also higher plants in natural product research, and its shortcomings and future prospects.

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Section: Spcas9mentioning

confidence: 99%

Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges

Biswas

Anand

Ghorai

et al. 2022

Biotechnology Journal

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“…This plant not only serves as a rich source of numerous antioxidant compounds but is also harnessed for its therapeutic potential in managing neurodegenerative diseases such as Parkinson's and Alzheimer's disease [19]. These therapeutic attributes have propelled it into the spotlight of numerous scientific investigations, including in vitro studies involving cell cultures, callus cultures, as well as tissue and organ cultures [20,21].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant and Cytotoxic Activities on Human Cervical Cancer Cells in Three Different Plant Extracts From Turkey

ULUSU

2023

Journal of Scientific Reports-A

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The objective of this investigation was to assess the total phenolic and flavonoid content within acetone and water extracts derived from Erica manipuliflora, Ferula communis, and Stevia rebaudiana plants. Additionally, the in vitro antioxidant and cytotoxic effects of these extracts were evaluated using the human cervical cancer (HeLa) cell line as a model. Among the three plant species examined, the highest concentrations of total phenols and flavonoids were found in the acetone extract of E. manipuliflora, measuring 365.29 mg GAE/g DW and 105.42 mg QE/g DW, respectively. Acetone and water extracts of E. manipuliflora showed higher DPPH scavenging activities (IC50: 37.57 and 33.27 µg/mL, respectively) compared to other plants. furthermore, E. manipuliflora acetone extract (IC50: 97.35 µg/mL) exhibited the highest inhibition in the HeLa cell line treated with the extracts, thus being the most effective extract on this cell line. As a result of the analyzes, it has been shown that F. communis and S. rebaudiana and especially E. manipuliflora are important natural antioxidant sources with their strong radical scavenging effects, as well as the anticancer potential of these plants.

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Investigating the Use of Biotechnologically Enhanced Medicinal Plants in Allergy Treatment

Mukherjee,

Dave,

Panigrahi

et al. 2024

Biotechnology of Medicinal Plants With Antiallergy Properties

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Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana

Cited by 16 publications

References 135 publications

Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges

Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges

In Vitro Evaluation of Antioxidant and Cytotoxic Activities on Human Cervical Cancer Cells in Three Different Plant Extracts From Turkey

Investigating the Use of Biotechnologically Enhanced Medicinal Plants in Allergy Treatment

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