Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy

Demling, Philipp; Baier, Makarius; Deitert, Alexander; Fees, Jana; Blank, Lars M

doi:10.1016/j.copbio.2024.103107

Cited by 9 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PolyP, due to their unique properties and relatively low cost of synthesis, nontoxicity, and biodegradability, are widely used in various industry (see above and [6,129]).…”

Section: Polyp As a Valuable Commoditymentioning

confidence: 99%

Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient

Solovchenko,

Plouviez,

Khozin-Goldberg

2024

Preprint

View full text Add to dashboard Cite

Phosphorus (P) is an important and irreplaceable macronutrient. It is central to energy and information storage and exchange in living cell. P is an element with “broken geochemical cycle” since it lacks abundant volatile compounds capable of closing the P cycle. P fertilizers are critical for global food security, but the reserves of minable P are scarce and non-evenly distributed between countries of the world. Accordingly, the risks of global crisis due to limited access to P reserves is expected to be graver than those entailed by competition for fossil hydrocarbons. Paradoxically, despite the scarcity and value of P reserves, its usage is extremely inefficient: current waste rate reaches 80% giving rise to a plethora of unwanted consequences such as eutrophication leading to harmful algal blooms. Microalgal biotechnology comprise a promising solution to tackle this challenge. The proposed review briefly presents the relevant aspects of microalgal P metabolism such as cell P reserve composition and turnover, and the regulation of P uptake kinetics for maximization of P uptake efficiency with the focus on novel knowledge. Multifaceted role of polyPhosphates, the largest cell depot for P, is discussed with emphasis on the P toxicity mediated by short-chain polyPhosphates. Opportunities and hurdles of P bioremoval via P uptake from waste streams with microalgal cultures, either suspended or immobilized, is discussed along with the role of bacterial components of microalgal-bacterial consortia in these processes. Possible avenues of P-rich microalgal biomass such as biofertilizer production or extraction of valuable polyPhosphates and other bioproducts are considered. The review concludes with a comprehensive assessment of the current potential of microalgal biotechnology for ensuring the sustainable usage of phosphorus.

show abstract

“…PolyP, due to their unique properties and relatively low cost of synthesis, nontoxicity, and biodegradability, are widely used in various industry (see above and [6,129]).…”

Section: Polyp As a Valuable Commoditymentioning

confidence: 99%

Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient

Solovchenko,

Plouviez,

Khozin-Goldberg

2024

Preprint

View full text Add to dashboard Cite

show abstract

Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7

Deitert,

Fees,

Mertens

et al. 2024

Yeast

View full text Add to dashboard Cite

Polyphosphate (polyP) is an intriguing molecule that is found in almost any organism, covering a multitude of cellular functions. In industry, polyP is used due to its unique physiochemical properties, including pH buffering, water binding, and bacteriostatic activities. Despite the importance of polyP, its analytics is still challenging, with the gold standard being 31P NMR. Here, we present a simple staining method using the fluorescent dye JC‐D7 for the semi‐quantitative polyP evaluation in yeast extracts. Notably, fluorescence response was affected by polyP concentration and polymer chain length in the 0.5–500 µg/mL polyP concentration range. Hence, for polyP samples of unknown chain compositions, JC‐D7 cannot be used for absolute quantification. Fluorescence of JC‐D7 was unaffected by inorganic phosphate up to 50 mM. Trace elements (FeSO4 > CuSO4 > CoCl2 > ZnSO4) and toxic mineral salts (PbNO3 and HgCl2) diminished polyP–induced JC‐D7 fluorescence, affecting its applicability to samples containing polyP–metal complexes. The fluorescence was only marginally affected by other parameters, such as pH and temperature. After validation, this simple assay was used to elucidate the degree of polyP production by yeast strains carrying gene deletions in (poly)phosphate homeostasis. The results suggest that staining with JC‐D7 provides a robust and sensitive method for detecting polyP in yeast extracts and likely in extracts of other microbes. The simplicity of the assay enables high‐throughput screening of microbes to fully elucidate and potentially enhance biotechnological polyP production, ultimately contributing to a sustainable phosphorus utilization.

show abstract

The role of inositol in the environmental organic phosphate cycle

Su,

Saiardi

2024

Current Opinion in Biotechnology

View full text Add to dashboard Cite

Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy

Cited by 9 publications

References 41 publications

Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient

Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient

Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7

The role of inositol in the environmental organic phosphate cycle

Contact Info

Product

Resources

About