Biotechnological Potential of Different Organs of Mistletoe (Viscum album L.) Collected from Various Host Tree Species in an Urban Area

Skrypnik, Liubov; Feduraev, Pavel; Golovin, Anton; Maslennikov, Pavel; Belov, Nikolay; Matveev, Matvei; Pungin, Artem

doi:10.3390/plants11202686

Cited by 15 publications

(8 citation statements)

References 55 publications

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“…The antioxidant activity of G. maritima and S. marina extracts was determined by the ability to capture the radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 -azino-bis (3ethylbenzthiazolino-6-sulfonic acid (ABTS) and by the reducing ability when reacting with Fe(III) -2,4,6-tripyridyl-s-triazine complex (FRAP) according to [44]. Antioxidant activity by DPPH, ABTS, and FRAP was determined spectrometrically using a microplate reader (CLARIOstar, BMG Labtech, Germany).…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

Effect of Salinity Stress on Phenolic Compounds and Antioxidant Activity in Halophytes Spergularia marina (L.) Griseb. and Glaux maritima L. Cultured In Vitro

Pungin

Lartseva

Loskutnikova

et al. 2023

Plants

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The study of halophytes as sources of phenolic compounds, as well as conditions that further enhance the accumulation of biologically active compounds in them, is of particular interest. In this paper, the effect of different salinity levels (25–500 mM in the form of NaCl) on the content of phenolic compounds and the antioxidant activity of two rare halophyte species Spergularia marina (L.) Griseb. and Glaux maritima L. cultured in vitro was investigated. A species-specific reaction of plants to salinization was established. In G. maritima, the maximum total content of phenolic compounds was observed at 50–100 mM, flavonoids 75–400 mM, and hydroxycinnamic acids 200–300 mM, as well as individual phenolics (protocatechuic acid, catechin, astragalin, hyperoside, rutin, isoquercitrin, and apigenin derivative) at 100–300 mM NaCl. For S. marina, on the contrary, there was a slight decrease in the content of phenolic compounds when NaCl was added to the nutrient medium compared to the control. The content of protocatechuic acid, rosmarinic acid, and apigenin derivative significantly decreased with increased salt stress. The change in antioxidant activity at different salinity levels was also species specific. The maximum values of different groups of phenolic compounds in G. maritima were observed at 50–300 mM NaCl. The cultivation of S. marina without the addition of NaCl and at 500 mM NaCl allowed the production of plants with the highest content of phenolic compounds. The obtained results can be further used in the development of protocols for the cultivation of these plants in vitro in order to induce the biosynthesis of phenolic compounds in them.

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Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

Effect of Salinity Stress on Phenolic Compounds and Antioxidant Activity in Halophytes Spergularia marina (L.) Griseb. and Glaux maritima L. Cultured In Vitro

Pungin

Lartseva

Loskutnikova

et al. 2023

Plants

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“…The amount of TPC in VA methanol extracts ranged from 9.81 to 22.76 mg GAE/g. It was determined that the amount of phenolic in the fruit extracts was higher than the branch and leaf extracts.Similarly, Skrypnik et al (2022) reported that TPC in mistletoe fruits was 3.0–3.2 times higher than in leaves and stems [55] …”

Section: Resultsmentioning

confidence: 91%

“…It was determined that the amount of ChemistrySelect phenolic in the fruit extracts was higher than the branch and leaf extracts.Similarly, Skrypnik et al (2022) reported that TPC in mistletoe fruits was 3.0-3.2 times higher than in leaves and stems. [55] The highest TPC was found in samples collected from Pyrus communis (22.76 mg GAE/g sample) at VA fruit methanol extracts. This was followed by Mespilus germanica (22.19 mg GAE/gfruit sample).…”

Section: Total Phenolic and Total Flavonoid Content Quantification An...mentioning

confidence: 90%

Determination of Antioxidant Activities of Viscum album L.: First Report on Interaction of Phenolics with Survivin Protein using in silico Analysis

et al. 2023

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Mistletoe, Viscum album L., (VA) known as “Ökse Otu” in Turkey, is a hemiparasitic shrub that grows especially on the trunks and crowns of broadleaf trees. This study is aimed to determination of the VA’s antioxidant compounds and evaluate their in vitro activities growing on twelve different host trees and determination of in silico approach of compounds against survivin protein. At the same time, the active ingredients determined in HPLC analysis, were performed with molecular docking and molecular dynamics analysis on survivin protein, an apoptosis inhibitor protein in cancer. Antioxidant activities of methanolic extracts of organs of VA were determined. Phytochemical properties of different organs of VA samples taken from different hosts varied as well. Total phenolic content was determined at the highest rate in fruits, followed by leaves and branches, respectively. Total flavonoid content was determined in the highest amounts in the leaves, followed by branches and fruits, respectively. Antioxidant activity values were evaluated with DPPH (using DPPH as a radical), ABTS (using ABTS as radical cation) and FRP tests. The antioxidant activity of the branches of the plant was determined at the highest rate, followed by leaves and fruits, respectively. During in silico analysis, Quercetin, Rosmarinic acid and Catechin were found to bind to survivin protein effectively. These substances can have the potential to become active pharmaceutical ingredients by working with different proteins in the future.

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“…The mismatch of the IR spectrum reaches up to 40% and this was often found in studies using plants as objects of study. 41,42 Analysis of proton ( 1 H) and carbon ( 13 C) NMR of standard and analytes was performed using a 99.9% (w/v; 0.36 M) solution standard of quercetin and analytes dissolved in methanol-d 4 were acquired using the JEOL JNM ECS-400 NMR spectrometer (Figure 5). This spectrum reveals three types of proton and carbon signals as follows: aromatic protons carbon and hydroxy protons carbon of the analyte and standard, and solvent proton carbon.…”

Section: Discussionmentioning

confidence: 99%

In vitro analysis of quercetin-like compounds from mistletoe Dendrophthoe pentandra (L.) Miq as a potential antiviral agent for Newcastle disease

Mochamad,

Malarvili,

Jasmine

et al. 2024

F1000Res

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Background Recent evidence suggests that some flavonoid compounds obtained from crude methanol extract of mistletoe leaves (Dendrophthoe pentandra L. Miq), also known as Benalu Duku (BD), have antimicrobial effects. Thus, the plant has the potential to eliminate viruses that may cause outbreaks in chicken farms. This study aimed to prove the in vitro ability of flavonoid compounds, namely quercetin-like compounds (QLCs), to eliminate field viruses, specifically the Newcastle disease virus (NDV). Methods This research was performed in two stages. An in vitro test was used with a post-test of the control groups designed at a significance of 0.05. BD leaves (5 kg) were extracted using a maceration method with methanol and then separated into hexane, chloroform, ethyl acetate, and methanol fractions. The final extracted products were separated using semi-preparative high-performance liquid chromatography (HPLC) to obtain QLCs. The QLCs were identified and compared with quercetin using HPLC, proton and carbon nuclear magnetic resonance spectrometry, Fourier transform infrared spectrophotometry and ultra-performance liquid chromatography-mass spectrometry. The activity of QLCs was tested in vitro against the NDV at a virulence titter of 10−5 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture. Results Solutions of 0.05% (w/v) QLCs were discovered to have antiviral activity against NDVs, with an average cytopathogenic effect antigenicity at a 10−5 dilution (p<0.05). Conclusions QLCs from flavonoids from the leaves of BD have in vitro antiviral bioactivity against NDV at a virulence titter of 10-5 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture. QLCs may have the potential to be developed as medicinal compounds for the treatment of other human or animal viral infections.

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Biotechnological Potential of Different Organs of Mistletoe (Viscum album L.) Collected from Various Host Tree Species in an Urban Area

Cited by 15 publications

References 55 publications

Effect of Salinity Stress on Phenolic Compounds and Antioxidant Activity in Halophytes Spergularia marina (L.) Griseb. and Glaux maritima L. Cultured In Vitro

Effect of Salinity Stress on Phenolic Compounds and Antioxidant Activity in Halophytes Spergularia marina (L.) Griseb. and Glaux maritima L. Cultured In Vitro

Determination of Antioxidant Activities of Viscum album L.: First Report on Interaction of Phenolics with Survivin Protein using in silico Analysis

In vitro analysis of quercetin-like compounds from mistletoe Dendrophthoe pentandra (L.) Miq as a potential antiviral agent for Newcastle disease

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