Biotechnological procedures to select white rot fungi for the degradation of PAHs

Lee, Hwanhwi; Jang, Yeongseon; Choi, Yong Seok; Kim, Min Ji; Lee, Jaejung; Lee, Hanbyul; Hong, Jeong Hee; Lee, Young Min; Kim, Gyu Hyeok; Kim, Jae Jin

doi:10.1016/j.mimet.2013.12.007

Cited by 133 publications

(91 citation statements)

References 27 publications

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“…Bacillus fusiformis isolated from wastewater sludge of an oil refinery showed optimal naphthalene degradation at pH 7 and 30°C [17]. Furthermore, among the four temperatures studied, 118 microbial isolates (78.6%) had an optimal temperature of 30°C for PAHs degradation, as previously reported [18] . Temperature and pH of the medium affected PAHs degradation by increasing the solubility of the compound [19] .…”

Section: (A) (A) (B) (D) (C)supporting

confidence: 81%

Proposal of possible pathway of fluorene biodegrada-tion by Citrobacter sp. FL5

Zhu

Huang

Zhang

et al. 2016

Appl Env Biotechnol

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Abstract:The biodegradation ability of Citrobacter sp. FL5 on fluorene was investigated in the present study. The bacterial isolate was identified based on biochemical test, physiological and 16S rDNA sequence analysis. Fluorene biodegradation was studied in the liquid media at the initial concentration of 50 mg L −1 fluorene at different pH values (6, 7, 8 and 9) and temperatures (25, 30, 37 and 45°C). Citrobacter sp. FL5 showed maximum performance of fluorene degradation ability at pH 7 and 30°C where it degrades 98% fluorene in liquid media. Furthermore, the isolate degrades 97.5% and 96% of 100 and 150 mg L -1 fluorene respectively at 168 hrs. The possible metabolic pathway for fluorene biodegradation by Citrobacter sp. FL5 was deduced by identification of metabolites through Gas Chromatography Mass Spectrometry (GC-MS) technique. 5 types of metabolites that were detected and identified were dibenzofuran, 9-fluorenylmethanol, methyl benzilate, piperonylic acid and catechol. This study was the first to report and describe the ability of Citrobacter sp. in detail for degradation of higher fluorene concentrations at various pH and temperatures ranges.

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Section: (A) (A) (B) (D) (C)supporting

confidence: 81%

Proposal of possible pathway of fluorene biodegrada-tion by Citrobacter sp. FL5

Zhu

Huang

Zhang

et al. 2016

Appl Env Biotechnol

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“…Tolerance is one of the properties evaluated when searching microorganisms for bioremediation processes (Bayman and Radkar 1997; Matsubara et al 2006; Lee et al 2014; Sharma et al 2017). A PCB tolerance test should be a primary stage in order to identify desirable PCB-degrading fungal species for biotechnological procedures.…”

Section: Resultsmentioning

confidence: 99%

“…Different screening techniques were carried out to select strains with polycyclic aromatic hydrocarbons, dyes and phenols biodegradation capabilities (Levin et al 2004; Matsubara et al 2006; Lee et al 2014; Carabajal et al 2016). However, plate screening has not been yet evaluated as a quick technique to select strains capable of tolerating and degrading PCBs.…”

Section: Introductionmentioning

confidence: 99%

Assessing the ability of white-rot fungi to tolerate polychlorinated biphenyls using predictive mycology

et al. 2018

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The aim of the present study was to assess the ability of different white-rot fungi to tolerate polychlorinated biphenyls (PCBs) using predictive mycology, by relating fungal growth inhibition to ligninolityc enzyme secretion. Fungal strains were grown in the presence of PCBs in solid media and their radial growth values were modelled through the Dantigny-logistic like function in order to estimate the time required by the fungal colonies to attain half their maximum diameter. The principal component analysis (PCA) revealed an inverse correlation between strain tolerance to PCBs and the laccase secretion over time, being laccase production closely associated with fungal growth capacity. Finally, a PCA was run to regroup and split between resistant and sensitive fungi. Simultaneously, a function associated with a model predicting the tolerance to PCBs was developed. Some of the assayed isolates showed a promising capacity to be applied in PCB bioremediation.Abbreviations: Polychlorinated biphenyls (PCBs), white-rot fungi (WRF)

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“…Several compounds, including crude oil, naphthalene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)pyrene (Da Silva, Umbuzeiro, Pfenning, Canhos, & Esposito, 2003;Argumedo-Delira, Alarcon, Ferreira-Cerrato, Almaraz, & Peña Cabriales, 2012;Lee et al, 2014;Zafra, Absálon, Cuevas, & Cortes-Espinosa, 2014), triazine herbicides (Colla, Primaz, Lima, Bertolini, & Costa, 2008) and heavy metals (Vale, Abreu, Gouveia, Leitão, & Santaella, 2011), has been used in these types of tests. The literature reports that high growth rates and tolerance to certain levels of contaminants are considered desirable characteristics in microorganisms, as are indicators of an ability to degrade such xenobiotics as PAHs (Lamar, Larsen, & Kirk, 1990;Matsubara, Lynch, & De Leij, 2006).…”

Section: Introductionmentioning

confidence: 99%

<b>Tolerance to Polycyclic Aromatic Hydrocarbons (PAHs) by filamentous fungi isolated from contaminated sediment in the Amazon region

Souza

Barreto

Mota

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Tolerance to Polycyclic Hydrocarbons Aromatic (PAHs) is considered an important characteristic when assessing the bioremediation potential of microorganisms. Given this, the objective of this research was to assay filamentous fungi from the Amazon region, isolated from sediments with differents levels of contamination by PAHs, for tolerance to phenanthrene and pyrene. To achieve this, fungal cultures plugs (5 mm), obtained after 7 days growth, were transferred to petri dishes containing 20% Sabouraud dextrose agar medium, after surface innoculation with phenanthrene and pyrene crystals, separately. Radial mycelial growth was evaluated after 10 days at five different concentration levels for each contaminant and control group, all in triplicate for each treatment. Fungal growth and growth inhibition rates were calculated. The average growth of the colonies in each treatment was compared with one-way ANOVA, followed by a Tukey Test (p < 0,05). All fungi showed tolerant to phenanthrene and pyrene. However, Hypoxylon sp. showed the lowest growth inhibition rate and average growth rates significantly different of the other six tested species. Hypoxylon sp. has been shown to be a promising genetic resource for use in new studies of PAHs degradation.Keywords: phenanthrene, pyrene, fungal growth inhibition, Amazon.Tolerância a Hidrocarbonetos Policíclicos Aromáticos (HPAs) por fungos filamentosos isolados de sedimentos contaminados da região Amazônica RESUMO. A tolerância a Hidrocarbonetos Policíclicos Aromática (HPAs) é considerada como uma característica importante na avaliação do potencial de micro-organismos para biorremediação. Diante disso, o objetivo desta pesquisa foi avaliar fungos filamentosos da região amazônica, isolados de sedimentos com diferentes níveis de contaminação por HPAs, quanto à tolerância ao fenantreno e pireno. Para tanto, discos das culturas fúngicas (5 mm), obtidas após 7 dias de crescimento, foram transferidas para placas de Petri contendo meio Agar Sabouraud Dextrose a 20%, após inoculação superficial com cristais de fenantreno e pireno, separadamente. O crescimento micelial radial foi avaliado após 10 dias em cinco concentrações diferentes para cada contaminante e grupo controle, ambos em triplicata para cada tratamento. As taxas de crescimento fúngico e de inibição de crescimento foram calculadas. O crescimento médio das colônias em cada tratamento foi comparado com ANOVA one way, seguido pelo teste de Tukey (p < 0,05). Todos os fungos mostraram tolerância ao fenantreno e ao pireno. No entanto, Hypoxylon sp. apresentou menor taxa de inibição de crescimento e taxas médias de crescimento significativamente diferentes das outras seis espécies testadas. Hypoxylon sp. tem se mostrado um recurso genético promissor para uso em novos estudos sobre degradação de HPAs.Palavras-chaves: fenantreno, pireno, inibição do crescimento fúngico, Amazônia.

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Biotechnological procedures to select white rot fungi for the degradation of PAHs

Cited by 133 publications

References 27 publications

Proposal of possible pathway of fluorene biodegrada-tion by Citrobacter sp. FL5

Proposal of possible pathway of fluorene biodegrada-tion by Citrobacter sp. FL5

Assessing the ability of white-rot fungi to tolerate polychlorinated biphenyls using predictive mycology

<b>Tolerance to Polycyclic Aromatic Hydrocarbons (PAHs) by filamentous fungi isolated from contaminated sediment in the Amazon region

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