Biotechnological Processes in Microbial Amylase Production

Gopinath, Subash C. B.; Anbu, Periasamy; Arshad, M. K. Md; Lakshmipriya, Thangavel; Voon, C. H.; Hashim, U.; Chinni, Suresh V.

doi:10.1155/2017/1272193

Cited by 167 publications

(95 citation statements)

References 32 publications

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“…Many of the Bacillus strains used commercially for the production of α-amylases have an optimum pH from 6 to 8 for the enzymatic production (Fooladi and Sajjadian, 2010;Wang et al, 2016). The influence of temperature on the production of amylase by bacteria is reported in the literature (Fooladi and Sajjadian, 2010;Dash et al, 2015;Wang et al, 2016;Gopinath et al, 2017). Konsoula and Liakopoulou-Kryiakides (2004) obtained the maximum production of amylase by thermophilic strains of Bacillus subtilis maintained at 40°C.…”

Section: Resultsmentioning

confidence: 99%

“…The amylases are indispensable in the food, textiles, paper, pharmaceutical, and detergent industries (Pandey et al, 2000;Sahnoun et al, 2015;Bhange et al, 2016;Al-Johani et al, 2016). The wide potential application of amylase in industrial and medicinal processes propels an effort on the part of researchers in seeking for the most efficient means of its production (Dash et al, 2015;Gopinath et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Besides, the level of production of this enzyme also differs depending on the source of the microbe, where isolates of environments rich in starch or amylose produce naturally greater quantities of the enzyme. Factors such as pH, temperature, salt, sources of carbon and nitrogen are vital to the amylase production rate Gopinath et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Research Article Genetic diversity of Bacillus sp producers of amylase isolated from the soil.

Xavier¹,

Lima

Oliveira

et al. 2017

Genet. Mol. Res.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Research Article Genetic diversity of Bacillus sp producers of amylase isolated from the soil.

Xavier¹,

Lima

Oliveira

et al. 2017

Genet. Mol. Res.

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“…Amylolytic activity was identified by Bacillus subtilis B19 [36]. Amylase production on the agar plate was proved by Aspergillus versicolor and Penicillium sp [37]. Amylase production has been done by numerous strains notably S. gulbargensis, Streptomyces strain A3, S. avermitilis, S. rochei BTSS 1001 [38][39].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Screening and Determination of Α-Amylase Activity From Marine Streptomyces Species

Sathya

Ushadevi

2018

Int J Pharm Pharm Sci

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Objective: This study was aimed to isolate potent amylase producing Streptomyces from the marine source.Methods: Soil samples were collected from less explored mangrove regions of Muthupet, Tamilnadu. Isolation of Streptomyces was performed by serial dilution plate technique using starch casein agar (SCA) (pH 7.2 and temp 28 °C). Morphological and biochemical characteristics were studied using Bergey's manual of systematic bacteriology. Preliminary screening and quantification of amylase activities were analysed in selected Streptomyces isolates by starch agar plate and dinitrosalicylic acid (DNS) method respectively.Results: Totally 65 isolates were separated from the marine soil. Among them, 23 strains showed different morphological features. These strains were subjected to amylase activity. Eight Streptomyces isolates (S1-S8) exhibited positive for amylase activity. The zone of clearance was exhibited in the range of diameters between 4-20 mm. Fermentation was prompted with inorganic salt starch agar, international Streptomyces project (ISP-4) media at 28 °C and incubated in an orbital shaker at 250 rpm for 96 h (pH 7.5). The quantitative estimation of amylase activity was exhibited selected eight isolates in the range between 2.4±0.002-5.9±0.005 (U/ml). The Streptomyces species S4, S5 and S6 exhibited strong amylase activity in both qualitative and quantitative level. Conclusion:This work motivating the amylase producing Streptomyces are originated in mangroves and it proved Streptomyces sp. S6 has a more efficient source of amylase production.

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“…The further support of energy consumption studies, such as those on thermodynamics, pinch technology, and environment-friendly technologies, has hastened the large-scale production of the enzyme [1]. Today a large number of extracellular microbial amylases are available commercially and they have almost completely replaced chemical hydrolysis of starch.…”

Section: Introductionmentioning

confidence: 99%

Maximum α-Amylase Production by Molecular and Biochemical Characterized Soil Microorganism

Singh¹,

Sharma²,

Singh³

2017

J Biotechnol Biomater

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The present investigation was undertaken with an aim to produce α-amylase in cost effective way keeping its market potential into consideration. This result obtained from present study prove that Bacillus licheniformis is the maximum α-amylase producing strain isolated from soil under suitable conditions. This study will very helpful for upcoming researchers to uncover the various aspect of enzyme production by microorganism. The present investigation was undertaken with an aim to produce higher amount of amylase with pure compounds in submerged fermentation. To screen the isolates for promising α-amylase producing capability and one of the most promising isolate is further scale up.Amylase constitutes a class of industrial enzymes having approximately 20% of the enzyme market. α-amylase has found its application in a range of industries including food, brewing, distilling industry, textile, paper pharmaceutical and bioconversion of solid waste etc. Microorganism used in present investigation, isolated from rhizospheric regions of giant newar variety of Raphanus sativus grown in district Jaunpur, (Uttar Pradesh) India. A total of 30 natural isolates were scanned for α-amylase activity out of which 20 isolates were biochemically characterized. Based on ribotyping all the isolates were identified and one of the most promising amylase producer, i.e., Bacillus licheniformis was finally selected for further studies. The experiments were carried out in triplicates and mean value was taken. Nutritional source such as carbon and nitrogen sources were optimized for the production of α-amylase in free cell condition. Maximum α-amylase production was observed at 24 h. of incubation. Three different types of media such as Semi-synthetic, complex media I and complex media II tested and various parameters were optimized for enhance the alpha-amylase yield. It was found that identified soil microorganism Bacillus licheniformis is the best strain for α-amylase production and complex media-I gave maximum yield.

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Biotechnological Processes in Microbial Amylase Production

Cited by 167 publications

References 32 publications

Research Article Genetic diversity of Bacillus sp producers of amylase isolated from the soil.

Research Article Genetic diversity of Bacillus sp producers of amylase isolated from the soil.

Isolation, Screening and Determination of Α-Amylase Activity From Marine Streptomyces Species

Maximum α-Amylase Production by Molecular and Biochemical Characterized Soil Microorganism

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