Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view

Cao, Yujin; Cao, Yugang; Zhao, Min’an

doi:10.1016/j.procbio.2012.05.011

Cited by 28 publications

(9 citation statements)

References 62 publications

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“…Although the precise mechanism by which some marine bacteria and low eukaryotes synthesize large amounts of EPA or DHA within the cell remains uncertain, a hypothetical pathway has been proposed (Wallis et al 2002 ; Napier 2002 ). Duplicating the aerobic pathway, biosynthesis via PKS also involves several rounds of reduction, dehydration, reduction, and condensation; in each cycle the fatty acyl chain is extended by a two-carbon unit while a double bond is introduced every three carbons along the acyl chain (Kaulmann and Hertweck 2002 ; Cao et al 2012 ). Several rounds of sequential reactions are repeated and result in the formation of LC-PUFA with 20 or 22 carbons and several double bonds that are methylene-interrupted, i.e., EPA or DHA.…”

Section: Lc-pufa Biosynthetic Pathwaysmentioning

confidence: 99%

Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA

Ruiz-López

Usher

Sayanova

et al. 2014

Appl Microbiol Biotechnol

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Omega-3 fatty acids are characterized by a double bond at the third carbon atom from the end of the carbon chain. Latterly, long chain polyunsaturated omega-3 fatty acids such as eicosapentaenoic acid (EPA; 20:5Δ5,8,11,14,17) and docosahexanoic acid (DHA; 22:6 Δ4,7,10,13,16,19), which typically only enter the human diet via the consumption of oily fish, have attracted much attention. The health benefits of the omega-3 LC-PUFAs EPA and DHA are now well established. Given the desire for a sustainable supply of omega-LC-PUFA, efforts have focused on enhancing the composition of vegetable oils to include these important fatty acids. Specifically, EPA and DHA have been the focus of much study, with the ultimate goal of producing a terrestrial plant-based source of these so-called fish oils. Over the last decade, many genes encoding the primary LC-PUFA biosynthetic activities have been identified and characterized. This has allowed the reconstitution of the LC-PUFA biosynthetic pathway in oilseed crops, producing transgenic plants engineered to accumulate omega-3 LC-PUFA to levels similar to that found in fish oil. In this review, we will describe the most recent developments in this field and the challenges of overwriting endogenous seed lipid metabolism to maximize the accumulation of these important fatty acids.

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Section: Lc-pufa Biosynthetic Pathwaysmentioning

confidence: 99%

Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA

Ruiz-López

Usher

Sayanova

et al. 2014

Appl Microbiol Biotechnol

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“…Also, as bacteria can be cultured and maintained almost indefinitely, they can be considered as a more easily renewable resource for the production of PUFA 2 . In addition, the PUFA production in PUFA-producing mutants generated from parent strains can be further enhanced by changing the composition of growth media and cultural conditions to make them better candidate for use in industry 21,22 .…”

Section: Discussionmentioning

confidence: 99%

Untitled

2015

IJPSR

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Polyunsaturated Fatty Acids (PUFA) have been shown to be of major importance in the prevention and treatment of a range of human diseases and disorders. A number of microorganisms have been shown to produce and accumulate PUFA. In the current study we have isolated 86 lipid producing microbes from various sources like a river from northern India, lakes from southern India and marine environments off the western coast of India. The isolates were shortlisted following a preliminary screening scheme by studying a set of parameters like intensity of lipid accumulation as judged by Sudan Black B staining, amount of biomass on nitrogen limited semi-synthetic medium, lipid quantity per gram biomass, relative quantities of saturated and unsaturated fatty acids and reproducibility of results. Lipid sample was extracted and derivatized for GC analysis using standard procedure. Further, GC/MS analysis confirmed that 5 isolates produce PUFA of omega-6(LA) and omega-3(ALA, ETA) type. Selected cultures have been identified by 16S r-RNA sequencing and are being optimized for PUFA production by the technique of fermentation.

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“…Bu durum siyanobakteriler, ökaryotik algler, mayalar ve küflerde tespit edilmiştir. EPA üretiminin de düşük sıcaklıklarda daha yüksek olduğu saptanmıştır (26) .…”

Section: Fiziksel Faktörlerunclassified

Single Cell oil Production from Microorganisms

Darcan¹,

Sarigül²

2016

TMCD

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Mikrobiyal yağlar (Single Cell Oil; SCO) hücrelerinde yağ biriktiren maya, küf, alg ve bakteriler tarafından üretilirler ve SCO üretimi uzun yıllardır endüstriyel ve bilimsel araştırma konusu olarak ilgi çekmektedir. Günümüzde tek hücre yağları gıda, ilaç ve bakım ürünlerindeki fonksiyonel yağların ve biyodizel üretiminde hammadde kaynağı olarak da önemlidir. Bitkisel ve hayvansal yağ kaynakları ile kıyaslandığında SCO'nun mikroorganizmalar tarafından üretilmesinin çeşitli avantajları vardır. Bu avantajlar; fermentasyon proseslerinin iklim koşullarından bağımsız olması, atık ürünlerin kullanılması ile gıda olarak da kullanılabilecek kaynaklarla rekabetten sakınılması ve çevre dostu üretim yapılmasıdır. Ayrıca "rekombinant biyoteknoloji" uygulamaları ile strain geliştirilmesi sonucunda yüksek hacimde, farklı, değerli ve ender bulunan tek hücre yağlarının üretilmesi sağlanmaktadır. Yüksek fermentasyon maliyetleri gelecekteki uygulamaları engellemektedir ve endüstriyel boyutta üretim potansiyeline şüpheli yaklaşılmasına neden olmaktadır. Bu nedenle çeşitli hidrofilik ve hidrofobik substratlar SCO üretimi için değerlendirilmektedir. SCO üretimi verimi karbon kaynağının tipi, azot kaynağı, sıcaklık, pH, süre, çözünmüş oksijen miktarı gibi çevresel koşullardan etkilenmektedir ve mikroorganizma türüne ve strainine bağlıdır. Tek hücre yağı üretiminde üretilen yağın miktarı ve yağ asidi kompozisyonu da çevresel koşullardan ve fermentasyon strainlerine bağlı olarak değişmektedir.

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Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view

Cited by 28 publications

References 62 publications

Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA

Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA

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Single Cell oil Production from Microorganisms

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