Biotechnological production of (R)-3-hydroxybutyric acid monomer

Tokiwa, Yutaka; Ugwu, Charles U.

doi:10.1016/j.jbiotec.2007.03.015

Cited by 95 publications

(62 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microbial production of (R)-3-hydroxybutyrate [(R)-3HB] is a typical example. This chiral compound is known as a monomeric constituent of bacterial polyester P(3HB), and is used as a precursor for synthetic antibiotics (Iimori and Shibasaki 1986), vitamins, pheromones, and also as a building block of biobased polyesters such as P(3HB) (Ren et al 2010, Tokiwa andUgwu 2007).…”

Section: Introductionmentioning

confidence: 99%

Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase

Matsumoto

Okei

Honma

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

(R)-3HB-CoA is hydrolyzed into (R)-3HB by modifying enzymes or undergoes 2 degradation of the polymerized product. In the present study, we constructed a new (R)-3HB-generating pathway from glucose by using propionyl-CoA transferase (PCT).This pathway was designed to excrete (R)-3HB by means of a PCT-catalyzed reaction coupled with regeneration of acetyl-CoA, the starting substance for synthesizing (R)-3HB-CoA. Considering the equilibrium reaction of PCT, the PCT-catalyzed (R)-3HB production would be expected to be facilitated by the addition of acetate since it acts as an acceptor of CoA. As expected, the engineered Escherichia coli harboring the phaAB and pct genes produced 1.0 g L -1 (R)-3HB from glucose, and with the addition of acetate into the medium, the concentration was increased up to 5.2 g L -1 , with a productivity of 0.22 g L -1 h -1 . The effectiveness of the extracellularly added acetate was evaluated by monitoring the conversion of 13 C carbonyl carbon labeled acetate into (R)-3HB using gas chromatography/mass spectrometry. The enantiopurity of (R)-3HB was determined to be 99.2% using chiral liquid chromatography. These results demonstrate that the PCT pathway achieved a rapid co-conversion of glucose and acetate into (R)-3HB.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase

Matsumoto

Okei

Honma

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Thus, PHAs have emrged as a promising class of new emerging bio-polymers in areas such as medicine, agriculture, tissue engineering, nanocomposites, polymer blends and chiral synthesis [255][256][257]. Significant improvement in properties of PHA can be achieved through co-polymerization.…”

Section: Genetically-engineered Polyestersmentioning

confidence: 99%

Challenges for Natural Monomers and Polymers: Novel Design Strategies and Engineering to Develop Advanced Polymers

Pillai

2010

Designed Monomers and Polymers

View full text Add to dashboard Cite

The last century witnessed the emergence of polymer science and technology that enabled the generation of knowledge and techniques to control the size, shape, structure, properties and functions of polymers to generate polymers with unprecedented properties and functions. There have been many attempts to apply this information to natural monomers and polymers to achieve the desired property profiles with varying degrees of success which have given/are giving it a new outlook as a possible alternative source for the production of polymers. Novel concepts and techniques such as 'bio-inspired' polymer design, 'synthetically-inspired' material development, etc., are contributing to the development of natural monomers and polymers as a sustainable resource to generate a knowledge-based design methodology to meet the vastly developing requirements of modern materials. This paper reviews these emerging concepts and techniques that integrate materials synthesis, process and manufacturing options with eco efficiency.

show abstract

“…They have also been used as a starting material in many pharmaceutical industries for the production of antibiotics, flavors, vitamins, and pheromones [3][4][5]. In addition, some of their derivatives exhibit antimicrobial, antiviral, and insecticidal activities [6][7][8]. To date, various methods, including the acid hydrolysis of biosynthetically produced poly(3-hydroxybutyrate), sharpless epoxidation and hydroxylation of allylic alcohols, and enantioselective reduction of 3-ketoesters [9][10][11], can be used to synthesize β-hydroxybutyric acid (β-HBA), which is one of the representative derivatives of β-hydroxy carboxylic acids.…”

Section: Introductionmentioning

confidence: 99%

Direct Conversion of Propylene Oxide to 3-Hydroxy Butyric Acid Using a Cobalt Carbonyl Ionic Liquid Catalyst

2017

View full text Add to dashboard Cite

show abstract

Biotechnological production of (R)-3-hydroxybutyric acid monomer

Cited by 95 publications

References 61 publications

Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase

Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase

Challenges for Natural Monomers and Polymers: Novel Design Strategies and Engineering to Develop Advanced Polymers

Direct Conversion of Propylene Oxide to 3-Hydroxy Butyric Acid Using a Cobalt Carbonyl Ionic Liquid Catalyst

Contact Info

Product

Resources

About