Biotechnological tools for detection, identification and management of plant diseases

Baştaş, Kubilay Kurtuluş; Boyraz, Nuh

doi:10.5897/ajb2018.16591

Cited by 10 publications

(4 citation statements)

References 49 publications

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“…By virtue of the speed, sensitivity, specificity and inherent simplicity, PCR has been extensively used for many kinds of applications, particularly revolutionized the field of diagnostics (Singh et al, 2014;Rajalakshmi 2017;Belete and Boyraz, 2019). PCR-based Method of Diagnosis/Identification can be broadly dived into two namely (a) when the sequence of nucleotide is known and (b) when it is unknown.…”

Section: Polymerase Chain Reaction (Pcr) Based Methodsmentioning

confidence: 99%

Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

Alemu

2020

Int J Appl Sci Biotechnol

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The second Sustainable Developmental Goals (SDG), among the seventeen SDG, is concerned with the pursuit of global food security and agricultural sustainability, which become the key to the success of the entire SDG. Whereas agricultural production and productivity are heavily threatened by the incidence of pests that inflict huge losses in various forms. This calls for prompt applications of biotechnology for the fast, accurate and reliable means for characterization of plant generic resources and pests as it is the pre-requisite and gateway for designing appropriate plant protection and production strategies. It is imperative that pests be identified properly so that judicious use of the literature can be made and sustainable management strategies can be implemented at the right stage. To this end, the application of biotechnology has made significant advances for reliable characterization of plant genetic resources as well as accurate diagnosis of pests, study of their genetic diversity and variability, detailed mechanisms of their transmission and evolutionary relationships. Accordingly, this review article covers the comprehensive account of the various molecular techniques, genome mapping and OMICS sciences utilized for characterization plants and pests that ultimately allow the detailed study of the biology and epidemiology of pests at any stage of their life cycle. The resulting data are eventually employed for enhancing successful implementation of sustainable plant protection and production strategies. In conclusion, the increasing projections of transboundary pests, environmental and abiotic factors together with the continuous scientific advancements and breakthroughs have made biotechnology to be an important engine of bioeconomy for generating invaluable products, processes and services. Int. J. Appl. Sci. Biotechnol. Vol 8(3): 247-288

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Section: Polymerase Chain Reaction (Pcr) Based Methodsmentioning

confidence: 99%

Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

Alemu

2020

Int J Appl Sci Biotechnol

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“…Depending on the symptoms, visual monitoring can be performed in the plant samples; it is frequently feasible to detect the primary pathogenesis of a worm, fungal spp., bacterial spp., or viruses [70]. Plant diseases can be identified by examining leaf curling, color change, and spotting of either brown or white color [71].…”

Section: Visual Observationmentioning

confidence: 99%

Scope of Onsite, Portable Prevention Diagnostic Strategies for Alternaria Infections in Medicinal Plants

et al. 2023

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Medicinal plants are constantly challenged by different biotic inconveniences, which not only cause yield and economic losses but also affect the quality of products derived from them. Among them, Alternaria pathogens are one of the harmful fungal pathogens in medicinal plants across the globe. Therefore, a fast and accurate detection method in the early stage is needed to avoid significant economic losses. Although traditional methods are available to detect Alternaria, they are more time-consuming and costly and need good expertise. Nevertheless, numerous biochemical- and molecular-based techniques are available for the detection of plant diseases, but their efficacy is constrained by differences in their accuracy, specificity, sensitivity, dependability, and speed in addition to being unsuitable for direct on-field studies. Considering the effect of Alternaria on medicinal plants, the development of novel and early detection measures is required to detect causal Alternaria species accurately, sensitively, and rapidly that can be further applied in fields to speed up the advancement process in detection strategies. In this regard, nanotechnology can be employed to develop portable biosensors suitable for early and correct pathogenic disease detection on the field. It also provides an efficient future scope to convert innovative nanoparticle-derived fabricated biomolecules and biosensor approaches in the diagnostics of disease-causing pathogens in important medicinal plants. In this review, we summarize the traditional methods, including immunological and molecular methods, utilized in plant-disease diagnostics. We also brief advanced automobile and efficient sensing technologies for diagnostics. Here we are proposing an idea with a focus on the development of electrochemical and/or colorimetric properties–based nano-biosensors that could be useful in the early detection of Alternaria and other plant pathogens in important medicinal plants. In addition, we discuss challenges faced during the fabrication of biosensors and new capabilities of the technology that provide information regarding disease management strategies.

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“…Therefore, different scientific seed health examinations and treatments are important to maintain seed health. Detection and identification of plant pathogens are among the most important strategies for sustainable plant disease management, including the availability of fast, sensitive, and accurate methods to improve disease control decision-making [2].…”

Section: Introductionmentioning

confidence: 99%

Detection of pathogens to test seed health of some food crops in storage

Suhendar,

Koswanudin,

Hidayatun

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The health condition of the seed, which is defined by the presence or absence of seed-borne microbes like fungi, bacteria, nematodes, or viruses, is one aspect that affects seed quality. The purpose of this study is to assess the health of some food crop seeds by looking for fungus and bacteria. The Pathogen Detection Laboratory, Division of Management of Genetic Resources (ICABIOGRAD), conducted this investigation in 2019. The Gen Bank collection and rejuvenation were the sources of the tested seeds. The use of moist blotter and agar procedures allowed for the detection of fungi and bacteria. 100 accessions of each of the following: rice, maize, soybeans, peanuts, sorghum, and cowpea had their seed health evaluated. Under a microscope, pathogens are observed growing, and their pathogenic properties are identified using the relevant literature. According to the findings, 12 maize accessions had fungal infections and 8 had bacterial infections. In peanuts, fungus and bacteria were found in 11 and 14 accessions, respectively. As opposed to 16 accessions of sorghum being infected with fungi and 18 accessions being infected with bacteria, nine accessions of soybeans had fungi and eleven accessions had bacteria. 18 accessions of the rice commodity had fungal infections, while 15 had bacterial infections. A total of 26 cowpea accessions had fungal infections, while 23 had bacterial infections. Even with relatively low infection rates (0–10%), fungus and bacterial infections on stored seeds are always present, with the exception of cowpea.

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Biotechnological tools for detection, identification and management of plant diseases

Cited by 10 publications

References 49 publications

Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

Scope of Onsite, Portable Prevention Diagnostic Strategies for Alternaria Infections in Medicinal Plants

Detection of pathogens to test seed health of some food crops in storage

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