Biotechnological valorization of biodiesel-derived glycerol: Trials with the non-conventional yeasts Yarrowia lipolytica and Rhodosporidium sp

Sarantou, Sofia; Stoforos, Nikolaos G.; Kalantzi, Ourania; Papanikolaou, Séraphim

doi:10.1016/j.crcon.2020.12.006

Cited by 50 publications

(23 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracellular metabolites, such as citric acid (16-20 g/L) and polyols (mannitol, erythritol and arabitol; 21-48 g/L), were produced as well. At the late growth phases, storage metabolites were re-consumed [31], similarly to other studies [11,[28][29][30][31][32] as well as the present study.…”

Section: Discussionsupporting

confidence: 91%

“…These values drop as the fermentation proceeds simultaneously with the increase of the values of cellular lipid (% in DCW). This is further evidence for a typical inter-play in the biosynthesis of the cellular polysaccharides and cellular lipids that has been reported above [28][29][30][31][32].…”

Section: Discussionsupporting

confidence: 85%

“…Some other studies have been performed that employed some oleaginous yeasts, e.g., Cryptococcus curvatus [28][29][30], Yarrowia lipolytica and Rhodosporidium sp. [31,32] for the production of lipids in different fermentation configurations and culture media. In non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium grown on biodiesel-derived glycerol as sole substrate, biomass production (DCW = 18.1-27.3 g/L) containing high amount of lipids (30.3-54.5% in DCW) was obtained, depending on the strain and media composition.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

et al. 2021

View full text Add to dashboard Cite

Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain R. kratochvilovae CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The ACA-DC 5033 wild-type strain (aka ACA-YC 5033 in the laboratory’s yeast collection), one of those isolated from acid wheat sourdough [ 136 ], exhibits a robust growth on crude glycerol and the advantageous capacity of being able to produce simultaneous high yields of citric acid and of lipids [ 24 ]. Proposed applications for this strain include the production of SCO, citric acid and polyols (mannitol, erythritol and arabitol) from biodiesel-derived crude glycerol [ 24 , 137 ]. Among the other food-derived isolates from this laboratory, the LMBF Y-46 strain, isolated from a Mediterranean fish ( Sparus aurata ), has demonstrated a remarkable capacity for growth on crude glycerol and production of high amounts of mannitol in a screening study involving 11 yeast strains from four genera (including six Y. lipolytica strains: ATCC 20460, ACA-DC 50109 and four other isolates of the laboratory) [ 197 ].…”

Section: Fantastic Yeasts and Where To Find Them: Yarrowia Strains And Yeast Collectionsmentioning

confidence: 99%

“…Collections-cf. [123], high citric acid production [124], sequenced strain [125] in situ soil bioremediation [51,52], citric acid production [124], UV-mutagenesis and metabolic engineering host for design of improved strains producing citrate or erythritol [70,[126][127][128] [129,130], robust growth on crude glycerol, simultaneous high lipid and citric acid yields [131] production of organic acids (notably citric acid) and SCO [129][130][131][132][133][134], including cocoa butter substitute [31,32], parent of evolved strain with increase oleaginicity [135], metabolic engineering host for design of improved GM strains [133,134] ACA-DC 5033 [aka ACA-YC 5033]/acid sourdough, Greece [136] ND wild-type prototroph robust growth on crude glycerol, simultaneous high lipid and citric acid yields [24] SCO, citric acid and polyol production [24,137] ATCC [141,142], self-cycling fermentation [143] organic acid production [143] H222/soil, Germany [10,144] (CLIB 80) MatA wild-type prototroph better fructose assimilation, high citric acid production [144], sequenced strain [34,37] organic acid production, metabolic engineering host for design of improved GM strains [145,146] NCI...…”

Section: Strain Usual Name/origin (Reference Number In Yeastmentioning

confidence: 99%

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

Madzak

2021

JoF

View full text Add to dashboard Cite

Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast Yarrowia lipolytica appears as one of the most attractive for a large range of white biotechnology applications, from heterologous proteins secretion to cell factories process development. The past, present and potential applications of wild-type, traditionally improved or genetically modified Yarrowia lipolytica strains will be resumed, together with the wide array of molecular tools now available to genetically engineer and metabolically remodel this yeast. The present review will also provide a detailed description of Yarrowia lipolytica strains and highlight the natural biodiversity of this yeast, a subject little touched upon in most previous reviews. This work intends to fill this gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide. At last, it will focus on exemplifying how advances in engineering tools can leverage a better biotechnological exploitation of the natural biodiversity of Yarrowia lipolytica and of other yeasts from the Yarrowia clade.

show abstract

Whey and post-frying oil as substrates in the process of microbial lipids obtaining: a value-added product with nutritional benefits

Wierzchowska

Derewiaka

Zieniuk

et al. 2023

Eur Food Res Technol

View full text Add to dashboard Cite

Yarrowia lipolytica has found many biotechnological applications. The species has a number of regulatory mechanisms to maintain cellular homeostasis, enabling biomass growth in complex media. The aim of this study was to evaluate the use of Y. lipolytica yeast as a platform for the simultaneous management of several industrial by-products and the production of microbial lipids with application potential in the chemical and food industries. Batch cultures of KKP 379 strain were conducted in media with post-frying rapeseed oil (PFO) and a by-product of curd cheese production—acid whey. To evaluate the potential of Yarrowia as a nutraceutical, quantitative and qualitative analyses of microbial sterols were carried out along with an assessment of the biomass mineral composition. It was indicated that the composition and content of sterols varied depending on the phase of cell growth in batch culture. During culture in medium with 20% (v/v) whey and 50 g/L PFO, the cellular lipid content reached 39% (w/w). The highest amount of sterols per dry biomass (7.38 mg/g) and cellular lipids (21.08 mg/g) was recorded after 38 h of culture. The dominant was ergosterol 12.10 mg/g (57%). In addition, the composition of carbon and nitrogen sources in the medium affected the content of selected elements in biomass, indicating that substrate modification can be a tool for manipulating the composition of yeast cells. The results of the study showed that the selection of waste substrates is an important factor in regulation of the cellular lipid accumulation efficiency, as well as the content of certain sterols.

show abstract

Biotechnological valorization of biodiesel-derived glycerol: Trials with the non-conventional yeasts Yarrowia lipolytica and Rhodosporidium sp

Cited by 50 publications

References 94 publications

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

Whey and post-frying oil as substrates in the process of microbial lipids obtaining: a value-added product with nutritional benefits

Contact Info

Product

Resources

About