Biotechnological Valorization of Food Marine Wastes: Microbial Productions on Peptones Obtained from Aquaculture By-Products

Abstract

Based on a biotechnological strategy, in the present work several peptones are produced from the Alcalase hydrolysis (0.1–0.2% v/w, 56–64 °C, pH 8.27–8.98, 3 h) and thermal processing (105 °C, 60 min) of wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass. These peptones were included (in the range of 2.6–11 g/L of soluble protein) as main source of organic nitrogen (protein substrates) in low-cost media for the culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria. In most cases, batch fermentations conducted in aquaculture peptone media led to the best growth, metabolic productions and yields. Nevertheless, no significant differences between aquaculture peptones and commercial media were generally observed. Kinetic parameters from a logistic equation and used for cultures modeling were applied with the purpose of comparing the bioproduction outcomes. In economical terms, the validity of the aquaculture peptones as substitutives of the peptones (meat extract, casitone, etc.) from commercial media was also compared. The decreasing of the costs for LAB bioproductions ranged between 3–4 times and the growth costs of MPB and Gram+ bacteria were improved more than 70 and 15 times, respectively, in relation to those found in control commercial media.

l‐Lactate oxidase‐mediated removal of l‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid

“…The high medium cost of the laboratory medium is mainly due to the cost for organic nitrogen sources. The medium cost for organic nitrogen sources in laboratory MRS medium, which contains 10 g L –1 beef extract, 10 g L –1 bacto peptone, and 5 g L –1 yeast extract, can be estimated at 2.71 US$ L –1 as the prices of beef extract, bacto peptone, and yeast extract are 100 US$ kg –1 , [ 27 ] 170.2 US$ kg –1[ 28 ] (calculated from 152 € kg –1 as 1€ is 1.12 US$), 1.25 US$ kg –1 , [ 29 ] respectively. In contrast, use of the CSL medium including 10 g L –1 CSL significantly reduced the cost for organic nitrogen source to 0.002 US$/L as the price of CSL is 0.2 US$/kg.…”

Valorization of Marine Waste: Use of Industrial By-Products and Beach Wrack Towards the Production of High Added-Value Products

“…Vázquez et al (2019a,b) described alternative processes to valorize fish discards and produce fish mince, gelatins, oils and fish protein hydrolysates to be used as aquaculture feed ingredients. Wastes generated from the industrial processing of various fish species can also be turned into peptones (watersoluble products of partial hydrolysis of proteins to be used as a liquid medium for growing bacteria) (Vázquez et al, 2020). Mussel meat is rich in protein, lipids, carbohydrates, minerals and carotenoid pigments (Grienke et al, 2014) with potential application as food/feed supplements, preservation agents and enzymes.…”

Circular Bio-economy—Paradigm for the Future: Systematic Review of Scientific Journal Publications from 2015 to 2021

“…When fishery wastes are hydrothermally treated, protein-rich peptones are obtained [93]. When these peptones were used as nitrogen sources to culture lactic acid bacteria (LAB) and marine probiotic bacteria (MPB), both of which find use in the food industry, researchers found that the production costs could be decreased by up to 75% and 98% respectively [94]. Bio-calcite-crab shells [92], oyster shells and gastropod shells-have been experimented with phosphorus adsorbents in wastewater treatment and for other value-added bio-products.…”