Biotechnological valorization of pineapple stem for pectinase production by Bacillus subtilis BKDS1: Media formulation and statistical optimization for submerged fermentation

Kavuthodi, Bijesh; Sebastian, Denoj

doi:10.1016/j.bcab.2018.05.003

Cited by 21 publications

(4 citation statements)

References 24 publications

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“…megaterium and these strains grew on this medium as well as on conventional Nutrient agar (Reddy et al, 2017). Kavuthodi et al (2015) applied 10% (w/v) watermelon rind extract to form liquid media containing 2.5 -15% (v/v) of this extract to culture B. subtilis BKDS1. The medium containing 12.5% the extract was optimal for the growth and the synthetization of exo-pectinase of B. subtilis BKDS1.…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Tran

Nguyen³

et al. 2023

Not Bot Horti Agrobo

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Plant-based media have recently been of interest as potential natural media for microbial culture. This study was conducted to apply inexpensive and available fruits to culture Bacillus antagonizing three fungal phytopathogens including Phytophthora capsici GTC 2.6.1, Rhizoctonia solani GTC 2.7.1, and Sclerotium rolfsii GTC 2.9.1. The results showed that the juice media of dragon fruit, Cavendish banana, watermelon, pineapple, and MT1 seedless guava could all be used to culture two screened antagonistic Bacillus strains including B08 and B18. Surveys on the media from watermelon juice indicated that juice concentration (based on mass of fruit pulp), initial pH, concentration of traditional medium added to the juice medium had different effects on the growth of two strains of Bacillus sp. B08 and B18. These preliminary results demonstrated the potential application of the juice in biomass production of antagonistic Bacillus strains. This approach is certainly safe for the environment and has the potential to improve fruit consumption and reduce the cost of microbial fertilizer production, so it needs more research attention.

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Section: Discussion Discussion Discussionmentioning

confidence: 99%

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Tran

Nguyen³

et al. 2023

Not Bot Horti Agrobo

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“…In this context, we emphasize the need to study l -asparaginase from endophytes and improve its synthesis. Moreover, response surface methodology (RSM) is one of the statistical methods that have been successfully used to optimize the growth conditions of organisms to increase overall metabolite and biomass production [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Response surface methodological optimization of l-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1

Abhini

Rajan

Zuhara

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background This study targets the enhanced production of l-asparaginase, an antitumor enzyme by Acinetobacter baumannii ZAS1. This organism is an endophyte isolated from the medicinal plant Annona muricata. Plackett–Burman design (PBD) and central composite design (CCD) were used for statistical optimization of media components. Results The organism exhibited 18.85 ± 0.2 U/mL enzyme activities in unoptimized media. Eight variables: l-asparagine, peptone, glucose, lactose, yeast extract, NaCl, MgSO4, and Na2HPO4 were screened by PBD. Among them, only four factors—l-asparagine, peptone, glucose, and Na2HPO4—were found to affect enzyme production significantly (p < 0.05). Furthermore, the best possible concentrations and interactive effects of the components that enhance this enzyme's output were chosen by using CCD on these selected variables. The results revealed that an optimized medium produces a higher concentration of enzymes than the unoptimized medium. After optimizing media components, the maximum l-asparaginase activity was 45.59 ± 0.36 U/mL, around the anticipated value of 45.04 ± 0.42 U/mL. After optimization of process parameters, it showed a 2.41-fold increase in the production of l-asparaginase by the endophyte Acinetobacter baumannii ZAS1. Conclusion The findings of this study indicated that an endophyte, Acinetobacter baumannii ZAS1 that produces l-asparaginase could be used to increase enzyme output. However, using the statistical methods Plackett-Burman design and central composite design of response surface methodology is a handy tool for optimizing media components for increased l-asparaginase synthesis.

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“…Innovative retting approaches, such as chemical, mechanical, osmotic, ultrasound, and microwave, were attempting to yield fiber; notwithstanding, every alternative has its impediments concerning pollution and significant expense [19][20][21]. The microbial solutions, for example, commencement of expected bacterial agents, either live strains or enzymes in the retting cycle, have demonstrated a plausible procedure yet request some basic conditions for their ideal initiation.…”

Section: Introductionmentioning

confidence: 99%

Critical Factors for Optimum Biodegradation of Bast Fiber’s Gums in Bacterial Retting

Hossain

Siddiquee

Subbiah

2021

Fibers

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Bast fiber plants require a post-harvest process to yield useable natural cellulosic fibers, denoted as retting or degumming. It encompasses the degradation of the cell wall’s non-cellulosic gummy substances (NCGs), facilitating fibers separations, setting the fiber’s quality, and determining downstream usages. Due to the inconvenience of traditional retting practices, bacterial inoculum and enzyme applications for retting gained attention. Therefore, concurrent changes of agroclimatic and socioeconomic conditions, the conventional water retting confront multiple difficulties, bast industries become vulnerable, and bacterial agents mediated augmented bio-retting processes trying to adapt to sustainability. However, this process’s success demands a delicate balance among substrates and retting-related biotic and abiotic factors. These critical factors were coupled to degrade bast fibers NCGs in bacterial retting while holistically disregarded in basic research. In this study, a set of factors were defined that critically regulates the process and requires to be comprehended to achieve optimum retting without failure. This review presents the bacterial strain characteristics, enzyme potentials, specific bast plant cell wall’s structure, compositions, solvents, and interactions relating to the maximum NCGs removal. Among plants, associated factors pectin is the primary biding material that determines the process’s dynamics, while its degree of esterification has a proficient effect through bacterial enzymatic degradation. The accomplished bast plant cell wall’s structure, macerating solvents pH, and temperature greatly influence the bacterial retting process. This article also highlights the remediation process of water retting pollution in a biocompatible manner concerning the bast fiber industry’s endurance.

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Biotechnological valorization of pineapple stem for pectinase production by Bacillus subtilis BKDS1: Media formulation and statistical optimization for submerged fermentation

Cited by 21 publications

References 24 publications

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Response surface methodological optimization of l-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1

Critical Factors for Optimum Biodegradation of Bast Fiber’s Gums in Bacterial Retting

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