Biotechnology—a sustainable alternative for chemical industry

Gavrilescu, Maria; Chisti, Yusuf

doi:10.1016/j.biotechadv.2005.03.004

Cited by 588 publications

(305 citation statements)

References 98 publications

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“…The global market for food and feed enzymes is a multibillion-dollar industry [49,50]. Enzymatic processing methods are widely used and collectively comprise an essential tool in the food-processing arena.…”

Section: Resultsmentioning

confidence: 99%

Treatment of cashew extracts with Aspergillopepsin reduces IgE binding to cashew allergens

2016

J App Biol Biotech

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Enzymes from Aspergillus fungal species are used in many industrial and pharmaceutical applications. Aspergillus niger and Aspergillus oryzae were cultured on media containing cashew nut flour to identify secreted proteins that may be useful as future food allergen processing enzymes. Mass-spectrometric analysis of secreted proteins and protein bands from SDS-PAGE gels indicated the presence of at least 63 proteins. The majority of these proteins were involved in carbohydrate metabolism, but there were also enzymes involved in lipid and protein metabolism. It is likely that some of these enzymes are specifically upregulated in response to cashew nut protein, and study of these enzymes could aid our understanding of cashew nut metabolism. Aspergillopepsin from A. niger was one of the proteolytic enzymes identified, and 6 distinct peptides were matched to this protein providing 22% coverage of the protein. Cashew extracts were incubated with a commercially available preparation of Aspergillopepsin (Acid Stable Protease, ASP) using simulated gastric fluid conditions to determine if ASP could degrade the protein and lower antibody binding to cashew allergens. Following treatment of cashew extract with ASP, a significant reduction was observed in cashew allergen binding to rabbit anti-cashew IgG using an immunoblot assay and serum IgE antibodies from cashew allergic individuals using competitive ELISA. These findings highlight the possible application of Aspergillopepsin/ASP from A. niger in food processing steps to attenuate cashew nut and other tree nut or peanut allergens.

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Section: Resultsmentioning

confidence: 99%

Treatment of cashew extracts with Aspergillopepsin reduces IgE binding to cashew allergens

2016

J App Biol Biotech

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“…There is currently significant effort under-way to extend pathway reconstructions to regions of metabolism that are poorly understood or to a large degree, have been functionally neglected (Breitling et al, 2008;Viswanathan et al, 2008). Industrial biotechnology has largely focused on the production of added value and commodity chemicals; however, the largest expected growth sector is in the area of specialty and fine chemicals, where industrial biotechnology offers simpler routes for complex synthetic chemistry, or the possibility of de novo chemicals that may offer similar or enhanced application (Gavrilescu and Chisti, 2005;Hirche, 2006). Specialty and fine chemical entities are typically present as metabolic intermediates in secondary and tertiary regions of metabolism, often poorly annotated, and rarely included in genome-scale network reconstructions.…”

Section: Discussionmentioning

confidence: 99%

“…However, the greatest percentage gain is likely to occur in the fine chemical market (16-60%), where industrial biotechnology platforms enable complex chemistry that are presently produced via complex synthetic or combinatorial routes (Hirche, 2006). Furthermore, industrial biotechnology is enabling new products including novel therapeutic agents such as polyketides, and specialty chemicals not previously identified such as the diverse polyunsaturated fatty acids and biopolymers produced by microalgae (Gavrilescu and Chisti, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…; annual market size exceeding US$1.5 billion), and amino acids (L-glutamate and L-lysine production by Corynebacterium glutamicum; annual production exceeding 600,000 tons) are well established and successful (Gavrilescu and Chisti, 2005). In each of these examples, host organisms well suited for production of the target compound were naturally isolated.…”

Section: Introductionmentioning

confidence: 99%

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Industrial systems biology

Otero

Nielsen

2009

Biotech & Bioengineering

134

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ABSTRACT:The chemical industry is currently undergoing a dramatic change driven by demand for developing more sustainable processes for the production of fuels, chemicals, and materials. In biotechnological processes different microorganisms can be exploited, and the large diversity of metabolic reactions represents a rich repository for the design of chemical conversion processes that lead to efficient production of desirable products. However, often microorganisms that produce a desirable product, either naturally or because they have been engineered through insertion of heterologous pathways, have low yields and productivities, and in order to establish an economically viable process it is necessary to improve the performance of the microorganism. Here metabolic engineering is the enabling technology. Through metabolic engineering the metabolic landscape of the microorganism is engineered such that there is an efficient conversion of the raw material, typically glucose, to the product of interest. This process may involve both insertion of new enzymes activities, deletion of existing enzyme activities, but often also deregulation of existing regulatory structures operating in the cell. In order to rapidly identify the optimal metabolic engineering strategy the industry is to an increasing extent looking into the use of tools from systems biology. This involves both x-ome technologies such as transcriptome, proteome, metabolome, and fluxome analysis, and advanced mathematical modeling tools such as genome-scale metabolic modeling. Here we look into the history of these different techniques and review how they find application in industrial biotechnology, which will lead to what we here define as industrial systems biology.

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“…Biofuels derived from algae have attained the importance in the past decades. Various types of sustainable and renewable fuels can be derived from microalgae, such as methane [10], biodiesel [16][17][18][19][20][21] and biohydrogen [22][23][24][25][26]. Microalgal species are ubiquitous that are able to survive in diverse habitat [27,28].…”

Section: Microalgae As Renewable Feedstock For Biofuelmentioning

confidence: 99%

Lucrative future of microalgal biofuels in Pakistan: a review

Manzoor

Tabssum

Javaid

et al. 2015

Int J Energy Environ Eng

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Pond-grown microalgae have been considered as feedstocks for production of biodiesel. Various natural habitats and man-made environments can be exploited to cultivate microalgae without utilizing arable land which is already decreasing due to urbanization and industrial development. Suitable temperature, solar illumination and availability of phosphorus and nitrogen-rich organic wastes result in algal blooms in stagnant water and river bank's associated pools in many regions including Pakistan.

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Biotechnology—a sustainable alternative for chemical industry

Cited by 588 publications

References 98 publications

Treatment of cashew extracts with Aspergillopepsin reduces IgE binding to cashew allergens

Treatment of cashew extracts with Aspergillopepsin reduces IgE binding to cashew allergens

Industrial systems biology

Lucrative future of microalgal biofuels in Pakistan: a review

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