Biotechnology and Molecular Approaches to Forage Improvement

Brummer, E. Charles; Wang, Zeng‐Yu

doi:10.1002/9781119436669.ch31

Cited by 2 publications

(2 citation statements)

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“…Certain forage species can also be used as turfgrass or as bioenergy crops. Transgenic technology has been employed to improve agronomical traits in a number of forage species (Brummer & Wang, 2020; Gou et al, 2019; Liu, Aung, et al, 2018; Z. Y. Wang & Brummer, 2012). Unlike transgenic technologies that require the stable integration of transgenes in the plant genome, genome editing allows the generation of targeted mutations with less safety issues.…”

Section: Introductionmentioning

confidence: 99%

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Application of CRISPR/Cas9 technology in forages

et al. 2022

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Genome editing is an advanced genetic modification tool that facilitates the removal, insertion, or substitution of nucleotides in the genome of an organism for mutation induction. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9) is the most widely used genome editing tool that can precisely modify the specific sequence of a genome. Significant progress has been made in the use of CRISPR/Cas9 for the genetic improvement of food crops. However, in forage crops, the use of CRISPR/Cas9 is still in the infant stage with limited understanding and application. This article reviews current advances in the establishment and application of the CRISPR/Cas9 system in alfalfa, summarizes the application of CRISPR/Cas9 in several other forage legume and grass species, and discusses the prospects of CRISPR/Cas9 in the genetic improvement of forage crops.

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Section: Introductionmentioning

confidence: 99%