Biotechnology and molecular biology of tropical and subtropical fruits

Islas-Osuna, María A.; Tiznado‐Hernández, Martín Ernesto

doi:10.1533/9780857093622.315

Cited by 4 publications

(2 citation statements)

References 307 publications

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“…Furthermore, the long regeneration phase can lead to morphological abnormalities and somaclonal variations [25,26]. In addition, many plant species are recalcitrant to in vitro regeneration [27][28][29]. In order to overcome these limitations and to achieve high transformation efficiencies, the nontissue culture in vivo method of Agrobacterium-mediated transformation, called in planta transformation has become increasingly important.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

Rizwan

Ahmed

Zhang

et al. 2021

Plants

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Passion fruit (Passiflora edulis) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies. In this study, a simple and efficient Agrobacterium-mediated in planta transformation system for passion fruit was established, using Agrobacterium virulent strain EHA105 harboring the binary vectors pCAMBIA1301 and pCAMBIA1302 with GUS and GFP reporter genes. The system requires less time and labor costs than conventional transformation systems, and no additional phytohormones and sterile conditions are required. Regeneration efficiency of 86% and transformation efficiency of 29% were achieved, when the wounds were wrapped with Parafilm and the plants were kept in darkness for 15 days. Approximately 75% of the regenerated plants had a single shoot and 26% multiple shoots. The transformation was confirmed at the DNA and RNA levels as well as by GUS staining and GFP fluorescent measurements. The developed protocol will contribute to the genetic improvement of passion fruit breeding.

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Section: Introductionmentioning

confidence: 99%

Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

Rizwan

Ahmed

Zhang

et al. 2021

Plants

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“…The development of next-generation sequencing (NGS) technologies, including second and third-generation sequencing, has signi cantly improved the genome sequencing of fruits (26). This progress has been instrumental in the development of genomics-assisted breeding programs, facilitated by the enhanced availability of genomic and transcriptomic data for various fruit species (27). RNA sequencing (RNA-Seq) is a powerful technology that enables researchers to study gene expression and transcriptomic data in a highthroughput manner.…”

mentioning

confidence: 99%

FEAtl: A Comprehensive web-based Expression Atlas for functional genomics in Tropical and Subtropical Fruit Crops

Roy,

Chaurasia,

Kumar

et al. 2024

Preprint

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Background Fruit crops, including tropical and subtropical fruits like Avocado (Persea americana), Fig (Ficus carica), Date Palm (Phoenix dactylifera), Mango (Mangifera indica), Guava (Psidium guajava), Papaya (Carica papaya), Pineapple (Ananas comosus), and Banana (Musa acuminata) are economically vital, contributing significantly to global agricultural output, as classified by the FAO's World Programme for the Census of Agriculture. Advancements in next-generation sequencing, have transformed fruit crop breeding by providing in-depth genomic and transcriptomic data. RNA sequencing enables high-throughput analysis of gene expression, and functional genomics, crucial for addressing horticultural challenges and enhancing fruit production. The genomic and expression data for key tropical and sub-tropical fruit crops is currently lacking a comprehensive expression atlas, revealing a significant gap in resources for horticulturists who require a unified platform with diverse datasets across various conditions and cultivars. Results The Fruit Expression Atlas (FEAtl), available at http://backlin.cabgrid.res.in/FEAtl/index.php, is a first-ever extensive and unified expression atlas for tropical and subtropical fruit crops developed using 3-tier architecture. The expressivity of coding and non-coding genes, encompassing 2,060 RNA-Seq samples across 91 tissue types and 177 BioProjects, it provides a comprehensive view of gene expression patterns for different tissues under various conditions. FEAtl features multiple tabs that cater to different aspects of the dataset, namely, Home, About, Analyze, Statistics, and Team and contains seven central functional modules: Transcript Information, Sample Information, Expression Profiles in FPKM and TPM, Functional Analysis, Genes Based on Tau Score, and Search for Specific Gene. The expression of a transcript of interest can be easily queried by searching by tissue ID and transcript type. Expression data can be displayed as a heat map, along with functional descriptions as well as Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Conclusions This atlas represents a groundbreaking compilation of a wide array of information pertaining to eight distinct fruit crops and serves as a fundamental resource for comparative analysis among different fruit species and is a catalyst for functional genomic studies.

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Current Status of Loquat (Eriobotrya JaponicaLindl.): Bioactive Functions, Preservation Approaches, and Processed Products

Dhiman

Suhag

Thakur

et al. 2021

Food Reviews International

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Biotechnology and molecular biology of tropical and subtropical fruits

Cited by 4 publications

References 307 publications

Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

FEAtl: A Comprehensive web-based Expression Atlas for functional genomics in Tropical and Subtropical Fruit Crops

Current Status of Loquat (Eriobotrya JaponicaLindl.): Bioactive Functions, Preservation Approaches, and Processed Products

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