Biotechnology of thermophilic bacteria — Growth, products, and application

Sonnleitner, Bernhard

doi:10.1007/bfb0004397

Cited by 53 publications

(36 citation statements)

References 249 publications

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“…Under oxygen-limited conditions, their metabolism switches to mixed fermentation in which alcohols and organic acids are formed (23,40,42). Since B. stearothermophilus strains are highly auxotrophic, they must be supplied with most amino acids and vitamins.…”

mentioning

confidence: 99%

“…For producing S-layer self-assembly products, the S-layer protein from cell wall fragments was extracted with guanidinium hydrochloride (GHCl) ( 40,000 x g at 20°C, most of the supernatant was dialyzed against distilled water at 4°C for at least 48 h. Portions (1 to 2 ml) of the GHCI extracts were dialyzed against either a KCl solution (20 mM in distilled water) or a CaCl2 solution (10 mM in distilled water) at 20°C. Samples from all preparations were examined by negative staining, which was done as previously described (25).…”

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confidence: 99%

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Isolation of two physiologically induced variant strains of Bacillus stearothermophilus NRS 2004/3a and characterization of their S-layer lattices

et al. 1994

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confidence: 99%

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confidence: 99%

Isolation of two physiologically induced variant strains of Bacillus stearothermophilus NRS 2004/3a and characterization of their S-layer lattices

et al. 1994

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“…Among the ten carbon sources studied, starch, sucrose, and lactose proved appreciably good for the protease production. Lactose, starch, soy meal and sucrose are considered good for industrial protease production (Sonnleitner, 1983). The appreciable high enzyme activity and stability at high temperatures and pH, suggest that K-30 can be a potential producer of alkaline protease by using cheap substances like wheat bran, rice bran, and soy meal.…”

Section: Resultsmentioning

confidence: 99%

Optimization of thermostable alkaline protease production from species of Bacillus using rice bran

Krishna¹,

Devi²

2005

Afr. J. Biotechnol.

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A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species. Isolate K-30 produced thermostable alkaline protease utilizing rice bran. The optimum conditions for protease activity was 55°C at pH 9 with 4% inoculum in the medium containing 1% rice bran after 96 h of incubation. Beef extract, tryptone and yeast extract were good nitrogen sources while lactose, starch, and sucrose were suitable for enzyme production. The extracellular production of the enzyme, its thermostable nature and compatibility with most commercial detergents are features which suggest its application in the detergent industry.

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“…Thermophilic bacteria are of particular interest in this respect, both in their own right, and as a source of desirable genes encoding highly active thermostable proteins for genetic constructions in mesophiles (Sonnleitner, 1983). Some 100 species of bacteria and fungi are known to produce cellulases (Ljungdahl & Eriksson, 1985) but comparatively little information is available regarding the biochemistry and genetic regulation of these important enzymes.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning of Clostridium Thermocellum DNA and the Expression of Further Novel Endo- ,4-glucanase Genes in Escherichia Coli

Romaniec

Clarke²,

Hazlewood³

1987

Microbiology

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Sau3A fragments of Clostridium thermocellum (NCIB 10682) DNA were ligated into the BamHI site of pBR322 and expressed in Escherichia coli HBlOl and a Lac-mutant thereof. Twentyeight clones with carboxymethylcellulase (CMCase) activity were selected from two libraries by means of the Congo Red plate assay. Restriction enzyme analysis indicated that the CMCase+ clones contained a total of 13 unique DNA inserts. Hybridization of recombinant plasmids with chromosomal DNA confirmed the physical maps in all but one case and was further used to demonstrate the absence of homology between the Hind111 restriction fragments of similar size which occurred in many of the clones. Without exception, CMCase+ E. coli clones expressed endoglucanase activity, but differed with respect to the amount and nature of the enzyme activity produced; additionally, some clones had exoglucanase activity which, in at least one case, was not attributable to the production of a second enzyme. For a few selected clones, the partially purified CMCase was analysed by electrophoresis. A temperature profile characteristic of a thermostable enzyme was demonstrated for the endoglucanase of one of the most active clones. Based on the evidence presented here, it is probable that the 13 unique DNA fragments described do not contain any of the C. thermocellum endoglucanase genes previously cloned.

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Biotechnology of thermophilic bacteria — Growth, products, and application

Cited by 53 publications

References 249 publications

Isolation of two physiologically induced variant strains of Bacillus stearothermophilus NRS 2004/3a and characterization of their S-layer lattices

Isolation of two physiologically induced variant strains of Bacillus stearothermophilus NRS 2004/3a and characterization of their S-layer lattices

Optimization of thermostable alkaline protease production from species of Bacillus using rice bran

Molecular Cloning of Clostridium Thermocellum DNA and the Expression of Further Novel Endo- ,4-glucanase Genes in Escherichia Coli

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