Biotecnología forestal aplicada a la producción de madera de nogal

Licea-Moreno, Ricardo Julián; Julián, Ricardo

doi:10.20868/upm.thesis.39525

Cited by 1 publication

(4 citation statements)

References 73 publications

(120 reference statements)

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“…It might suggest a differential exhaustion of culture medium components between laboratories, since higher rooting percentages and number of roots per microshoots were obtained in laboratory 1. Some other authors [34] have also observed that using the same culture medium and conditions, microshoots from vessels with more explants inoculated stopped growing earlier than those with lower quantities, appearing a general declination of microshoots, i.e., wilting and defoliation, by the likely exhaustion of culture medium components.…”

Section: Acclimatizationmentioning

confidence: 80%

“…The quality of microshoots at the end of root expression was also higher when these were cultured in DWKC variant (Figure 3a); producing the microshoots from MS shorter roots than in DKWC. Additionally, microshoots from MS culture medium were more affected by defoliation than those from DKWC (Figure 3b), which might represent a disadvantage during hardening, as has been observed by McGranahan et al [52] and Licea-Moreno [34]. During the 80's, MS was widely used for walnut micropropagation [37,39,53], being replaced by DKW after its creation [10].…”

Section: Rootingmentioning

confidence: 92%

“…Here, a differential degree of defoliation at the end of root expression was observed, depending of the origin of microshoots, with greater affectations for those that grew in culture medium MS than in DKWC. Both McGranahan et al [52] and Licea-Moreno [34] have observed that defoliated rooted-microshoots have less possibilities to overcome ex vitro hardening. Regardless of the differences caused for culture media once the plants were acclimated, the phenotypic variations almost disappeared as can be observed in Figure 4b,c.…”

Section: Acclimatizationmentioning

confidence: 99%

“…Solar et al [33] have registered similar time course profiles for accumulation of phenolic substances of several Persian walnut varieties, being increased the production of flavonoids from the beginning of the growth cycle in May to the end of August. Hence, a large reduction of phenolics production and the number of dead explants has been registered for walnut hybrids and Persian walnut introduced during winter [34,35]. As genotype cannot be controlled, choice of the right moment of introduction might increase the success of in vitro establishment.…”

Section: In Vitro Introduction and Establishmentmentioning

confidence: 99%

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Unraveling Factors Affecting Micropropagation of Four Persian Walnut Varieties

Yegizbayeva¹,

García-García

Yausheva³

et al. 2021

Agronomy

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Walnuts are considered recalcitrant to tissue culture, with a great genetic determinism on all stages of micropropagation; while other factors, also with great impact, become more complicated with the reproduction of newly realized varieties. In this research, a holistic approach aimed to determine the influence of genotype and the nutritive formulation throughout the whole cycle of micropropagation of four Persian walnut varieties (Juglans regia L.) was presented. During the in vitro establishment it was determined that besides genotype and culture medium, the effect of collection season and the likely interaction amongst factors had a great influence on the successful introduction of all four genotypes. However, all cultures were affected by a deep decay, being necessary the introduction of ethylenediamine di-2-hydroxyphenyl acetate ferric, as iron source, and Phloroglucinol in both Murashige and Skoog (1962) and the corrected Driver and Kuniyuki (1987) formulations. These modifications allowed the stabilization of cultures, maintaining thereafter a steady quality. Either proliferation, rooting and ex vitro survival of four clones were affected by the culture medium, obtaining the best results with the corrected Driver and Kuniyuki (1987) formulation. Finally, in vitro plants produced from all clones were acclimated with high survival rates (75.9–91.1% for the best culture medium), depending of clone and the culture medium used. Microsatellite analysis showed that micropropagated plants maintained the same genetic profiles of their corresponding donor trees. These results might contribute to deepening of the understanding of factors that determine the success of micropropagation of walnuts, and the extents of its influence; whereas, it sets the basis for the commercial micropropagation of all four clones.

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Section: Acclimatizationmentioning

confidence: 80%