dCover crops provide several ecosystem services, but their impact on enteric bacterial survival remains unexplored. The influence of cover cropping on foodborne pathogen indicator bacteria was assessed in five cover crop/green manure systems: cereal rye, hairy vetch, crimson clover, hairy vetch-rye and crimson clover-rye mixtures, and bare ground. Cover crop plots were inoculated with Escherichia coli and Listeria innocua in the fall of 2013 and 2014 and tilled into the soil in the spring to form green manure. Soil samples were collected and the bacteria enumerated. Time was a factor for all bacterial populations studied in all fields (P < 0.001). E. coli levels declined when soil temperatures dipped to <5°C and were detected only sporadically the following spring. L. innocua diminished somewhat but persisted, independently of season. In an organic field, the cover crop was a factor for E. coli in year 1 (P ؍ 0.004) and for L. innocua in year 2 (P ؍ 0.011). In year 1, E. coli levels were highest in the rye and hairy vetch-rye plots. In year 2, L. innocua levels were higher in hairy vetch-rye (P ؍ 0.01) and hairy vetch (P ؍ 0.03) plots than in the rye plot. Bacterial populations grew (P < 0.05) or remained the same 4 weeks after green manure incorporation, although initial reductions in L. innocua numbers were observed after tilling (P < 0.05). Green manure type was a factor only for L. innocua abundance in a transitional field (P < 0.05). Overall, the impacts of cover crops/green manures on bacterial population dynamics in soil varied, being influenced by bacterial species, time from inoculation, soil temperature, rainfall, and tillage; this reveals the need for long-term studies.
In agricultural environments, soil can serve as a reservoir and route of transmission for foodborne pathogens (1). Since fresh produce is often consumed raw without a "kill step" to inactivate human-pathogenic microorganisms, it is important to prevent the contamination of these foods during production (2). Growers may implement good agricultural practices (GAPs) to minimize the risk of produce contamination at the preharvest level (3), as this program focuses on on-farm risk factors, including animalbased fertilizers, irrigation water quality, farm worker training and hygiene, and wildlife exclusion. Outbreaks and contamination events, however, continue to occur, emphasizing the need to evaluate the role of other agricultural practices, which thus far have received less attention, on enteric pathogen dynamics on a farm.One such practice is cover cropping, the establishment of a crop, typically a small grain or legume, in between cultivations of a cash crop. In recent years, economic and environmental considerations have renewed interest in this old practice for improving crop productivity and soil health and maintaining the sustainability of agroecosystems (4). Cover cropping brings a variety of ecosystem services to agricultural systems, including seasonal protection of soil from erosion, weed suppression, soil improvement, a...