Biotic stress-induced and ripening-related anthocyanin biosynthesis are regulated by alternate phytohormone signals in blueberries

Günther, Catrin S.; Plunkett, Blue; Cooney, Janine M.; Jensen, Dwayne J.; Trower, Tania; Elborough, Caitlin; Nguyen, Han M.; Deng, Cecilia; Lafferty, Declan J.; Albert, Nick W.; Dare, Andrew P.; Espley, Richard V.

doi:10.1016/j.envexpbot.2022.105065

Cited by 11 publications

(3 citation statements)

References 64 publications

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“…A Vaccinium fruit transient assay system such as the non-anthocyanic blueberry flesh, would be ideal for verifying the functionality of Vaccinium anthocyanin structural genes, particularly for ANS . However, this has been proven difficult due to stress-induced anthocyanin biosynthesis when blueberry fruit is injected with Agrobacterium during the transient expression process ( Günther et al., 2022 ). Nonetheless, our experimental findings for the function of VmANS and especially, VcUFGT2 in modulating anthocyanin concentrations in heterologous transient expression systems ( N. benthamiana leaf and strawberry fruit) suggested fundamental key roles for these enzymes in the biosynthesis pathway.…”

Section: Discussionmentioning

confidence: 99%

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

et al. 2023

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Anthocyanins are a major group of red to blue spectrum plant pigments with many consumer health benefits. Anthocyanins are derived from the flavonoid pathway and diversified by glycosylation and methylation, involving the concerted action of specific enzymes. Blueberry and bilberry (Vaccinium spp.) are regarded as ‘superfruits’ owing to their high content of flavonoids, especially anthocyanins. While ripening-related anthocyanin production in bilberry (V. myrtillus) and blueberry (V. corymbosum) is regulated by the transcriptional activator MYBA1, the role of specific structural genes in determining the concentration and composition of anthocyanins has not been functionally elucidated. We isolated three candidate genes, CHALCONE SYNTHASE (VmCHS1), ANTHOCYANIDIN SYNTHASE (VmANS) and UDP-GLUCOSE : FLAVONOID-3-O-GLYCOSYLTRANSFERASE (VcUFGT2), from Vaccinium, which were predominantly expressed in pigmented fruit skin tissue and showed high homology between bilberry and blueberry. Agrobacterium-mediated transient expression of Nicotiana benthamiana showed that overexpression of VcMYBA1 in combination with VmANS significantly increased anthocyanin concentration (3-fold). Overexpression of VmCHS1 showed no effect above that induced by VcMYBA1, while VcUFGT2 modulated anthocyanin composition to produce delphinidin-3-galactosylrhamnoside, not naturally produced in tobacco. In strawberry (Fragaria × ananassa), combined transient overexpression of VcUFGT2 with a FLAVONOID 3´,5´-HYDROXYLASE from kiwifruit (Actinidia melanandra) modulated the anthocyanin profile to include galactosides and arabinosides of delphinidin and cyanidin, major anthocyanins in blueberry and bilberry. These findings provide insight into the role of the final steps of biosynthesis in modulating anthocyanin production in Vaccinium and may contribute to the targeted breeding of new cultivars with improved nutritional properties.

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Section: Discussionmentioning

confidence: 99%

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

et al. 2023

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“…Anthocyanin accumulation is affected by multiple biotic and abiotic factors, such as nutrition, temperature, light and wounding ( Weiss, 2000 ; Lin-Wang et al., 2011 ; Günther et al., 2022 ). Low temperature can induce anthocyanin biosynthesis and the expression of related genes, while high temperature accelerates anthocyanin degradation ( Rowan et al., 2009 ; Rehman et al., 2017 ) and is unfavorable for anthocyanin synthesis ( Crifò et al., 2012 ; Ryu et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

High temperature inhibited the accumulation of anthocyanin by promoting ABA catabolism in sweet cherry fruits

Tan

Wen

et al. 2023

Front. Plant Sci.

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Color is an essential appearance characteristic of sweet cherry (Prunus avium L.) fruits and mainly determined by anthocyanin. Temperature plays an important role in the regulation of anthocyanin accumulation. In this research, anthocyanin, sugar, plant hormone and related gene expression were analyzed using physiological and transcriptomic methods in order to reveal the effects of high temperature on fruit coloring and the related mechanism. The results showed that high temperature severely inhibited anthocyanin accumulation in fruit peel and slowed the coloring process. The total anthocyanin content in fruit peel increased by 455% and 84% after 4 days of normal temperature treatment (NT, 24°C day/14°C night) and high temperature treatment (HT, 34°C day/24°C night), respectively. Similarly, the contents of 8 anthocyanin monomers were significantly higher in NT than in HT. HT also affected the levels of sugars and plant hormones. The total soluble sugar content increased by 29.49% and 16.81% in NT and HT, respectively, after 4 days of treatment. The levels of ABA, IAA and GA20 also increased in both the two treatments but more slowly in HT. Conversely, the contents of cZ, cZR and JA decreased more rapidly in HT than in NT. The results of the correlation analysis showed that the ABA and GA20 contents were significantly correlated with the total anthocyanin contents. Further transcriptome analysis showed that HT inhibited the activation of structural genes in anthocyanin biosynthesis as well as the repression of CYP707A and AOG, which dominated the catabolism and inactivation of ABA. These results indicate that ABA may be a key regulator in the high-temperature-inhibited fruit coloring of sweet cherry. High temperature induces higher ABA catabolism and inactivation, leading to lower ABA levels and finally resulting in slow coloring.

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“…Meanwhile, v-myb avian myeloblastosis viral oncogene homolog (MYB), basic helix-loop-helix (bHLH), WD-repeat protein (WD40), and other transcription factors can affect the synthesis of anthocyanins by regulating the expression of structural genes [17][18][19]. Furthermore, environmental factors also impact the accumulation of anthocyanins in plants [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings

Wang

Yuan

et al. 2023

Plants

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Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RILpur–W22 and RILbro–W22) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RILpur–W22 were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RILpur–W22) provided candidate genes for further genetic analysis.

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Biotic stress-induced and ripening-related anthocyanin biosynthesis are regulated by alternate phytohormone signals in blueberries

Cited by 11 publications

References 64 publications

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

High temperature inhibited the accumulation of anthocyanin by promoting ABA catabolism in sweet cherry fruits

Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings

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