Biotin–avidin amplified enzyme-linked immunosorbent assay for determination of isoflavone daidzein

He, Jiantao; Shi, Zhihong; Jin, Yan; Zhao, Meiping; Guo, Zhiqiang; Chang, Wen‐Bao

doi:10.1016/j.talanta.2004.07.027

Cited by 25 publications

(14 citation statements)

References 17 publications

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“…4). Compared with reported ELISA assay for determining daidzein [15], the method described here has almost the same sensitivity.…”

Section: Resultsmentioning

confidence: 98%

“…Furthermore, immunoassays have become popular in recent years due to their advantages of high throughput screening purposes, potential of high sensitivity and specificity, and relatively low cost. He et al [15] reported a biotin-avidin-amplified enzyme-linked immunosorbent assay (ELISA) for determining daidzein in serum, urine, and P. radix. BennetauPelissero et al [16] also reported ELISA to measure genistein and daidzein in food and human fluids.…”

Section: Introductionmentioning

confidence: 99%

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Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

et al. 2010

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Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4',7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

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“…4). Compared with reported ELISA assay for determining daidzein [15], the method described here has almost the same sensitivity.…”

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

et al. 2010

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“…In the case of ELISA it is necessary to prepare antibodies (monoclonal or polyclonal) against analyzed isoflavones or their conjugates (antigens) between isoflavones and a bovine serum albumin (BSA) or other high-molecular carrier (for details see [74]). Samples are transferred onto a microtitration plate and the antibodies obtained are bound to antigens present in the sample.…”

Section: Immunoanalysismentioning

confidence: 99%

Current trends in isolation, separation, determination and identification of isoflavones: A review

Vacek

Klejdus

Lojková

et al. 2008

J of Separation Science

108

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Isoflavones are natural substances which elicit a number of physiological effects in living organisms. The substances are synthesized in plant tissues as protective agents against biotic stress (i. e. bacterial infection). Isoflavones are also an important dietary constituent in human nutrition. Modern trends in studies of isoflavones in plant materials and foodstuffs and procedures for chemical analyses of isoflavones in human body fluids and plant tissues are discussed in this review. Highly effective extraction and purification techniques, i. e. solid-phase extraction, accelerated-solvent extraction, and Soxhlet extraction, are presented. Latest procedures in chromatographic separation of isoflavones that apply different types of sorbents are described. Immunochemical analysis, electrochemical sensing of isoflavones, and spectrometric and other analytical techniques and their applications are also mentioned. Special attention is paid to the highly selective and sensitive technique of mass spectrometry and its application for identification of isoflavones and their glucosides in plants. Studies of interactions of isoflavones with cell receptors and a number of biologically active substances such as DNA and proteins are described. The review does not intend to give a complete overview of the topics considered but rather to present modern and most recent methods used in studies of isoflavones.

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“…The extraction and purification of abscisic acid (ABA), indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR) and gibberellic acid (GA) were conducted as described in He et al (2005) and Dong et al (2008) ELISA kits using monoclonal antibodies (Phytodetek, Agdia, Elkhart, IN, USA) were used for reactions with the extracts following the procedure provided by the manufacturer. All samples were measured at 405 nm (Ye et al, 2012).…”

Section: Determination Of Hormone Concentrationmentioning

confidence: 99%

Effects of CuO nanoparticles on insecticidal activity and phytotoxicity in conventional and transgenic cotton

et al. 2016

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Biotin–avidin amplified enzyme-linked immunosorbent assay for determination of isoflavone daidzein

Cited by 25 publications

References 17 publications

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Current trends in isolation, separation, determination and identification of isoflavones: A review

Effects of CuO nanoparticles on insecticidal activity and phytotoxicity in conventional and transgenic cotton

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