Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy

Purushothaman, Baskaran; Choi, Jinsung; Park, Sol-Ji; Lee, Jeongmin; Samson, Annie Agnes Suganya; Hong, Sera; Song, Joon Myong

doi:10.1039/c8tb01923a

Cited by 63 publications

(68 citation statements)

References 33 publications

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“…Based on the layer-by-layer seed mediated shell growth strategy, UCNPs were obtained with pure hexagonal phase ( Figure 1A) and homogeneous particles sizes ( Figure 1B). Based on the modified water-in-oil reverse microemulsion method (Purushothaman et al, 2019), dense silica was homogeneously grown on the surface of UCNPs with a thickness of 6.2-8.3 nm ( Figure 1C) with the finial zetapotential of −5.6 mv ( Figure 1D). During the silanization, PS molecules could be easily incorporated into the silica layer without further modification with a concentration of 19.6-26.8 µg/mg, which was confirmed by the UV-Vis analysis ( Figure S2A) and zeta-potential ( Figure S2B).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, ICG as PS molecules and NIR sensitizer could effectively enhance PDT efficacy. The short half-life (<40 ns) and restricted action distance (<20 nm) of the generated ROSs usually limit the therapeutic efficacy of PDT (Thomas et al, 2017;Purushothaman et al, 2019). In this study, under the drive of mitochondria-targeting ligands, the obtained nanotheranostic agent would selectively accumulate in the mitochondria where ROSs were produced under the excitation of NIR laser and then the mitochondria-mediated cell apoptosis was induced (Scheme 1).…”

Section: Introductionmentioning

confidence: 87%

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Construction of Multicolor Upconversion Nanotheranostic Agent for in-situ Cooperative Photodynamic Therapy for Deep-Seated Malignant Tumors

et al. 2020

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Upconversion nanoparticles (UCNPs)-based photodynamic nanotheranostic agents could address the main drawbacks of photosensitizer molecules (PSs) including instability in aqueous solution and rapid clearance. Due to the relatively weak luminescence intensity of UCNPs and insufficient reactive oxygen species (ROSs), UCNPs-based photodynamic therapy (UCNPs-PDT) was discounted for deep-seated tumors. Thus, we proposed a PSs-modulated sensitizing switch strategy. Indocyanine green (ICG) as an NIR organic dye was proved to effectively enhance the luminescence intensity of UCNPs. Herein, four-color UCNPs were coated with a silica layer which loaded ICG and PSs while the thickness of silica layer was controlled to assist the sensitization function of ICG and activation of PSs. Under the drive of mitochondria-targeting ligand, the prepared nanotheranostic agent would accumulate in the mitochondria where ROSs were in-situ produced and then cell apoptosis was induced. Due to the cooperative PDT and high tissue-penetration depth of NIR laser, the prepared upconversion nanotheranostic agent could achieve significant inhibition on the deep-seated tumors.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Construction of Multicolor Upconversion Nanotheranostic Agent for in-situ Cooperative Photodynamic Therapy for Deep-Seated Malignant Tumors

et al. 2020

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“…The Generation Efficiency of Singlet Oxygen for UiO‐66‐TCPP : Through a classical method, DPBF was utilized to quantify the generation efficiency of singlet oxygen . The certification of the DPBF′s stability was of great importance to avoid its disturbance to this test.…”

Section: Methodsmentioning

confidence: 99%

Mitochondria‐Targeted Nanoscale MOFs for Improved Photodynamic Therapy

et al. 2019

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Due to the existence of hypoxic microenvironment, the efficacy of photodynamic therapy (PDT) is frequently weakened. As a result, targeted treatment toward oxygenrich mitochondria is considered as a promising cancer therapy. Herein, both triphenylphosphine (TPP) and folic acid (FA) are simultaneously grafted onto nanoscale metal-organic frameworks (NMOFs) to realize dual-targeting delivery of the nanoplatforms into cancer cells and their mitochondria via the proposed phosphorylation modification strategy. A large amount of highly efficient photosensitizer of porphyrinic molecules is integrated into the NMOFs with a uniform particle size of about 65 nm. Thanks to the strong ZrÀ OÀ P bonding, a dense coverage of phosphonate-conjugating targeting molecules on NMOFs is obtained and each surficial unsaturated ZrÀ O cluster is adequately occupied. The resultant dual-targeting NMOFs feature high biostability and biocompatibility, as well as improved cellular uptake and mitochondrial accumulation. The PDT efficacy of these dualtargeting NMOFs is significantly improved with a low IC 50 of 0.74 μM upon 10 min light radiation, which is at least four times higher than that of non-targeting counterparts. This phosphorylation strategy would be hopeful for immobilizing a variety of biogenic groups on NMOFs to make them targetable to various specific organelles and to improve the therapy efficacy on related diseases.

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“…Particularly, their strong affinities to transferrin is known to cause more accumulation in cancer cells than in normal cells due to an active metabolism that needs more Fe 2+ ion [10,11]. TPP-PEG-biotin is a PDT substance that produces reactive oxygen species (ROSs) by irradiation at 660 nm [12]. Due to the covalent bonding of TPP to PEG, this material can act as self-assembly-based NDDS and a photosensitizer.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional TPP-PEG-biotin self-assembled nanoparticle drug delivery-based combination therapeutic approach for co-targeting of GRP78 and lysosome

et al. 2020

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Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy

Abstract: In this study, the chemo-drug doxorubicin (DOX) was successfully encapsulated in PEG–biotin conjugated porphyrin SANs (DOX@TPP–PEG–biotin) and had synergistic effects after PDT action.

Cited by 63 publications

References 33 publications

Construction of Multicolor Upconversion Nanotheranostic Agent for in-situ Cooperative Photodynamic Therapy for Deep-Seated Malignant Tumors

Construction of Multicolor Upconversion Nanotheranostic Agent for in-situ Cooperative Photodynamic Therapy for Deep-Seated Malignant Tumors

Mitochondria‐Targeted Nanoscale MOFs for Improved Photodynamic Therapy

Multifunctional TPP-PEG-biotin self-assembled nanoparticle drug delivery-based combination therapeutic approach for co-targeting of GRP78 and lysosome

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