Biotin‐decorated redox‐responsive micelles from diselenide‐linked star‐shaped copolymers for the targeted delivery and controlled release of doxorubicin in cancer cells

Abstract

Polymeric micelles (s-PMs) assembled from star-shaped amphiphilic copolymers functionalized with cancer-homing molecules have been shown to have enormous potential to be used as a delivery tool to achieve longer half-life in the bloodstream, cancer-specific internalization, and controlled release of payloads in tumor cells. Considering the unprecedented thermodynamic stability of s-PMs owing to the entanglement of branched arms, we prepared a biotin-decorated and diselenide-linked star-shaped amphiphilic copolymer, 3s-PCL-SeSe-PEG-biotin, which could undergo self-directed clustering to form uniformly distributed s-PMs with a hydrodynamic diameter (D h ) of 76.27 nm.The s-PMs had appreciable doxorubicin (DOX)-loading content (DLC) and encapsulation efficiency (EE) of 5.83 wt% and 71.02%, respectively. The blank and DOX-loaded 3s-PCL-SeSe-PEG-

Advances in active targeting of ligand-directed polymeric nanomicelles via exploiting overexpressed cellular receptors for precise nanomedicine

“…In another study, star-shaped redox-sensitive polymeric micelles were assembled from 3s-PCL-SeSe-PEG amphiphilic copolymer using ring opening reaction. 117 Biotin was further attached via its carboxylic group to the amino moiety of 3s-PCL-SeSe-PEG via DCC/NHS conjugation technique, then DOX was integrated into the targeted micellar core using dialysis or solvent exchange reaction. Physicochemical studies showed that the prepared nanomicelles were stable upon high dilution with redox-sensitive drug release profile in the high cancer redox environment.…”

Actively Targeting Redox-Responsive Multifunctional Micelles for Synergistic Chemotherapy of Cancer

“…The intersection point of the vertical and horizontal tangent lines of the curve through the points of low concentration is considered as the CMC of the copolymer. 26,31 2.7. Preparation of Blank and DOX-Loaded Micelles.…”

“…It is based on the dilution of the micellar solution against a large volume of solvent, usually PBS, which represents the volume of the entire systemic circulation. 31 Thus, a 5 mg/mL micellar solution was prepared, and the volume was adjusted to 1000 folds by diluting with PBS. Then, the change in hydrodynamic diameter (D h ) as a function of time was measured in triplicate every 24 h for 7 days.…”

“…Encapsulation of DOX within Bi-(HOOC−PEG-PLGA)-S 2 and Bi(Dig−PEG-PLGA)-S 2 micelles could improve the solubility and minimize systemic toxicity during therapeutic usage. 31 In this study, the CMC of the Bi(Dig−PEG-PLGA)-S 2 copolymer was estimated using a fluorescence spectrophotometer, employing pyrene as the hydrophobic core probe. The CMC of the copolymer was found to be 0.014 mg/mL (Figure S2b), indicating that Bi(Dig−PEG-PLGA)-S 2 copolymer-based micelles might be stable at extreme dilution and could have longer circulation times without premature release of its cargo.…”

“…53−55 Overall, the DLC and EE of Bi(Dig−PEG-PLGA)-S2 were more acceptable as compared with disulfide-linked redoxresponsive systems designed for DOX delivery. 31,56 3.6. Redox-Responsive Drug Releasing Behavior.…”