Biotin-dependent expression of the asialoglycoprotein receptor in HepG2.

Collins, Janna C.; Paietta, Elisabeth; Green, R; Morell, Anatol G.; Stockert, Richard J.

doi:10.1016/s0021-9258(18)37954-7

Cited by 59 publications

(8 citation statements)

References 22 publications

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“…The use of biotin in these studies reflects the fact that the expression levels of the asialoglycoprotein receptor (ASGP-R) in HepG2 cells are generally low 20 but can be induced by biotin treatment. 21 Consistent with such literature reports and fully in accord with our design expectations, the fluorescence intensity of 1 in HepG2 cells was found to depend on the concentration of biotin in the cellular medium (Figure S20). Moreover, fluorescence changes were seen in the case of the other cell lines noted above.…”

Section: ■ Results and Discussionsupporting

confidence: 91%

Hepatocyte-Targeting Single Galactose-Appended Naphthalimide: A Tool for Intracellular Thiol Imaging in Vivo

et al. 2012

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We present the design, synthesis, spectroscopic properties, and biological evaluation of a single galactose-appended naphthalimide (1). Probe 1 is a multifunctional molecule that incorporates a thiol-specific cleavable disulfide bond, a masked phthalamide fluorophore, and a single galactose moiety as a hepatocyte-targeting unit. It constitutes a new type of targetable ligand for hepatic thiol imaging in living cells and animals. Confocal microscopic imaging experiments reveal that 1, but not the galactose-free control system 2, is preferentially taken up by HepG2 cells through galactose-targeted, ASGP-R-mediated endocytosis. Probe 1 displays a fluorescence emission feature at 540 nm that is induced by exposure to free endogenous thiols, most notably GSH. The liver-specificity of 1 was confirmed in vivo via use of a rat model. The potential utility of this probe in indicating pathogenic states and as a possible screening tool for agents that can manipulate oxidative stress was demonstrated in experiments wherein palmitate was used to induce lipotoxicity in HepG2 cells.

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Section: ■ Results and Discussionsupporting

confidence: 91%

Hepatocyte-Targeting Single Galactose-Appended Naphthalimide: A Tool for Intracellular Thiol Imaging in Vivo

et al. 2012

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“…PL is a water-soluble, nonionic polysaccharide [108] which has a low toxicity and immunogenicity [107]. PL binds to the asialoglycoprotein receptor [109] which is overexpressed on HepG2 cells with maximum receptor activity [110]. The cellular uptake results from this study showed that the NPs internalized far better in HepG2 cells than Hela cells, due to the high asialoglycoprotein receptor overexpression in HepG2 cells, confirming the effective targeting ability of this nanocomposite [107].…”

Section: Porphyrin-based Magnetic Nanoparticlessupporting

confidence: 62%

Recent Advances in Porphyrin-Based Inorganic Nanoparticles for Cancer Treatment

Montaseri

Kruger

Abrahamse

2020

IJMS

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The application of porphyrins and their derivatives have been investigated extensively over the past years for phototherapy cancer treatment. Phototherapeutic Porphyrins have the ability to generate high levels of reactive oxygen with a low dark toxicity and these properties have made them robust photosensitizing agents. In recent years, Porphyrins have been combined with various nanomaterials in order to improve their bio-distribution. These combinations allow for nanoparticles to enhance photodynamic therapy (PDT) cancer treatment and adding additional nanotheranostics (photothermal therapy—PTT) as well as enhance photodiagnosis (PDD) to the reaction. This review examines various porphyrin-based inorganic nanoparticles developed for phototherapy nanotheranostic cancer treatment over the last three years (2017 to 2020). Furthermore, current challenges in the development and future perspectives of porphyrin-based nanomedicines for cancer treatment are also highlighted.

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“…30 They found that the expression of the asialoglycoprotein receptor is reduced in biotin-deficient HepG2 cells. 30 Addition of biotin or biocytin restored full expression of the asialoglycoprotein receptor, while messenger RNA expression was not modified. Additional support to the notion that biotin affects protein expression was obtained in high-throughput immunoblotting studies.…”

Section: Biotin As Gene Expression Regulatormentioning

confidence: 99%

“…27 Biotin also modifies gene expression at the posttranscriptional level. 30,31 The earliest studies pointing to a role of biotin in post-transcriptional events were by Stockert and colleagues and date back to the late 1980s. 30 They found that the expression of the asialoglycoprotein receptor is reduced in biotin-deficient HepG2 cells.…”

Section: Biotin As Gene Expression Regulatormentioning

confidence: 99%

“…30,31 The earliest studies pointing to a role of biotin in post-transcriptional events were by Stockert and colleagues and date back to the late 1980s. 30 They found that the expression of the asialoglycoprotein receptor is reduced in biotin-deficient HepG2 cells. 30 Addition of biotin or biocytin restored full expression of the asialoglycoprotein receptor, while messenger RNA expression was not modified.…”

Section: Biotin As Gene Expression Regulatormentioning

confidence: 99%

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Biological Effects of Pharmacological Concentrations of Biotin

Fernández-Mejía

Lazo-de-la-Vega-Monroy

2011

J Evid Based Complementary Altern Med

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Understanding the molecular mechanisms of vitamins has opened new perspectives regarding the relationship between nutritional signals and biological functions, which, in turn, has led to the development of new therapeutic agents. Although little is known about water-soluble vitamins as genetic modulators, evidence about their effects on gene expression has grown. In the case of biotin, besides its role as a carboxylase prosthetic group, it also affects gene expression and has a wide repertoire of effects on biological functions. Only recently, the role of pharmacological concentrations of biotin on systemic functions has attracted attention, and it is now being reconsidered with the help of new technologies. This novel approach could lead to new perspectives in its use as a therapeutic agent. The present review is focused on the effects of pharmacological concentrations of biotin on several biological functions and on the biotin signaling pathways that participate in gene expression.

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Biotin-dependent expression of the asialoglycoprotein receptor in HepG2.

Cited by 59 publications

References 22 publications

Hepatocyte-Targeting Single Galactose-Appended Naphthalimide: A Tool for Intracellular Thiol Imaging in Vivo

Hepatocyte-Targeting Single Galactose-Appended Naphthalimide: A Tool for Intracellular Thiol Imaging in Vivo

Recent Advances in Porphyrin-Based Inorganic Nanoparticles for Cancer Treatment

Biological Effects of Pharmacological Concentrations of Biotin

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