Biotin-dependent regulation of gene expression in human cells

León-Del-Río, Alfonso

doi:10.1016/j.jnutbio.2005.03.021

Cited by 17 publications

(8 citation statements)

References 16 publications

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“…Availability of vitamins has been shown to affect the expression of a variety of genes in different cellular systems (2,7,9,16); however, there is limited knowledge regarding the effect of pyridoxine availability on global gene expression. Although an effect of pyridoxine availability on expression of total RNA in PACs (22) and a few selected genes (amylase and trypsin) has been reported (4), a comprehensive analysis of the whole transcriptome under conditions of altered pyridoxine level has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile

Srinivasan

Ramesh

et al. 2019

American Journal of Physiology-Cell Physiology

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Pyridoxine (vitamin B6), an essential micronutrient for normal cell physiology, plays an important role in the function of the exocrine pancreas. Pancreatic acinar cells (PACs) obtain vitamin B6 from circulation, but little is known about the mechanism involved in the uptake process; limited information also exists on the effect of pyridoxine availability on the gene expression profile in these cells. We addressed both these issues in the current investigation using mouse-derived pancreatic acinar 266-6 cells (PAC 266-6) and human primary PACs (hPACs; obtained from organ donors), together with appropriate physiological and molecular (RNA-Seq) approaches. The results showed [3H]pyridoxine uptake to be 1) pH and temperature (but not Na+) dependent, 2) saturable as a function of concentration, 3) cis-inhibited by unlabeled pyridoxine and its close structural analogs, 4) trans-stimulated by unlabeled pyridoxine, 5) regulated by an intracellular Ca2+/calmodulin-mediated pathway, 6) adaptively-regulated by extracellular substrate (pyridoxine) availability, and 7) negatively impacted by exposure to cigarette smoke extract. Vitamin B6 availability was found (by means of RNA-Seq) to significantly (FDR < 0.05) modulate the expression profile of many genes in PAC 266-6 cells (including those that are relevant to pancreatic health and development). These studies demonstrate, for the first time, the involvement of a regulatable and specific carrier-mediated mechanism for pyridoxine uptake by PACs; the results also show that pyridoxine availability exerts profound effects on the gene expression profile in mammalian PACs.

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Section: Discussionmentioning

confidence: 99%

Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile

Srinivasan

Ramesh

et al. 2019

American Journal of Physiology-Cell Physiology

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“…It can be responsible for transporting biotin into cells through a specific cell membrane receptor [ 15 , 16 ]. In addition, biotin-transferase activity of BTD in modification of genes expression by biotinylation of histones was shown [ 17 - 20 ]. Biotinidase activity is particularly high in liver, adrenal gland and kidney [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…This explains its obligatory involvement in the metabolism of carbohydrates, lipids and deaminated residues of some amino acids. Moreover, another biotin function as gene expression regulator was reported recently [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study

et al. 2009

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“…First, Leon-Del-Rio and co-workers suggested that holocarboxylase synthetase plays a critical role in biotin-dependent signaling by cGMP and PKG (22, 42). A possible involvement of holocarboxylase synthetase in the regulation of NO and NOS has not been investigated in this study.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Signaling Depends on Biotin in Jurkat Human Lymphoma Cells

Rodriguez-Melendez

Zempleni

2009

The Journal of Nutrition

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Biotin affects gene expression through a diverse array of cell signaling pathways. Previous studies provided evidence that cGMP-dependent signaling also depends on biotin, but the mechanistic sequence of cGMP regulation by biotin is unknown. Here we tested the hypothesis that the effects of biotin in cGMP-dependent cell signaling are mediated by nitric oxide (NO). Human lymphoid (Jurkat) cells were cultured in media containing deficient (0.025 nmol/L), physiological (0.25 nmol/L), and pharmacological (10 nmol/L) concentrations of biotin for 5 wk. Both levels of intracellular biotin and NO exhibited a dose-dependent relationship in regard to biotin concentrations in culture media. Effects of biotin on NO levels were disrupted by the NO synthase (NOS) inhibitor N-monomethyl-arginine. Biotin-dependent production of NO was linked with biotin-dependent expression of endothelial and neuronal NOS, but not inducible NOS. Previous studies revealed that NO is an activator of guanylate cyclase. Consistent with these previous observations, biotin-dependent generation of NO increased the abundance of cGMP in Jurkat cells. Finally, the biotin-dependent generation of cGMP increased protein kinase G activity. Collectively this study is consistent with the hypothesis that biotin-dependent cGMP signaling in human lymphoid cells is mediated by NO.

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Biotin-dependent regulation of gene expression in human cells

Cited by 17 publications

References 16 publications

Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile

Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile

Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study

Nitric Oxide Signaling Depends on Biotin in Jurkat Human Lymphoma Cells

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