Biotin‐Functionalized Cellulose‐Based Monolayers as Sensitive Interfaces for the Detection of Single Molecules

Jung, Stefan; Angerer, Bernhard; Löscher, Frank; Niehren, Stefan; Winkle, Johannes; Seeger, Stefan

doi:10.1002/cbic.200500338

Cited by 17 publications

(15 citation statements)

References 31 publications

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“…Biotin-functionalized microcrystalline cellulose mono layers could be used to detect single molecules after specifi c binding onto a streptavidin coating. [ 7 ] Dong et al developed a three-step reaction pathway to covalently attach fl uorescent fl uorescein-5 ′ -isothiocyanate (FITC) mole cules to the surface of cellulose nanocrystals. [ 6 ] These fl uorescently labeled cellulose nanocrystals enable the use of fl uorescence techniques, such as bioimaging applications.…”

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confidence: 99%

Synthesis and Fluorescent Properties of Carbazole‐Substituted Hydroxyethylcelluloses

Zhang

Zhou

Zhang

2012

Macro Chemistry & Physics

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Carbazole-substituted hydroxyethylcelluloses (Cz-HECs) are homogeneously synthesized by reacting hydroxyethylcellulose (HEC) with N -3 ′ -bromopropyl carbazole (Br-Cz). The structure of Cz-HECs is characterized with elemental analysis and NMR, and the DS of the carbazole is in the range 0.11-0.43, determined from nitrogen content. The fl uorescent properties of Cz-HECs are measured using a spectrofl uorimeter. The results show that the fl uorescence lifetime increases as the DS increases, and the fl uorescent intensity of Cz-HECs is stronger than that of Br-Cz. All samples exhibit concentration self-quenching properties. The addition of acrylonitrile, as an electron acceptor, quenches the emission spectra of Cz-HECs, and the fl uorescence quenching is found to be a dynamic quenching by analyzing with the Stern-Volmer equation.

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confidence: 99%

Synthesis and Fluorescent Properties of Carbazole‐Substituted Hydroxyethylcelluloses

Zhang

Zhou

Zhang

2012

Macro Chemistry & Physics

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“…Particularly, cellulose has been employed in the manufacture of dialysis membranes due to their high hydrophilicity [10]. Cellulose membranes have been biofunctionalized by carboxybetaines for improving blood compatibility [11] or by chimeric avidin [12] to be used as sensor with even single molecule sensitivity [13]. The chemical nature of both types of membranes renders them excellent support materials for biotechnological and medical applications [14].…”

Section: Introductionmentioning

confidence: 99%

Membrane surface functionalization via theophylline derivative coating and streptavidin immobilization

Hierrezuelo¹,

Romero²,

Benavente³

et al. 2014

Colloids and Surfaces B: Biointerfaces

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“…Therefore, unspecific adsorption of proteins is often effectively suppressed and smooth cellulose surfaces show a low‐fouling behavior 7, 8. These low levels of unspecific binding allowed for instance the application of cellulose thin films as support for the detection of single molecules 9, 10. Thin films of cellulose, deposited on solid and transparent supports made of glass or plastics are therefore promising for the development of DNA or protein microarrays and bioanalytical lab‐on‐a‐chip devices.…”

Section: Introductionmentioning

confidence: 99%

Functional Patterning of Biopolymer Thin Films Using Enzymes and Lithographic Methods

Kargl

Mohan

Köstler

et al. 2012

Adv Funct Materials

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Two different lithographic techniques for the patterning of thin biopolymer films are developed. The first method is based on using a microstructured elastomeric mold for the structuring of thin films of regenerated cellulose. The thin films are manufactured by spin‐coating of trimethylsilyl cellulose (TMSC) and subsequent regeneration. The microchannels formed by the mold and the cellulose film are filled with a cellulase solution by capillary action. In the areas exposed to the enzyme solution, the cellulose film is digested, whereas the area in contact with the mold is protected from the enzymatic activity. Optical thickness measurements, atomic force microscopy and fluorescent staining confirm a successful patterning of cellulose on several substrates by this method. The second method is based on the structured regeneration of thin TMSC films. TMSC surfaces are protected with metal masks and exposed to vapors of hydrochloric acid. These treatments result in hydrophilic cellulose structures surrounded by hydrophobic TMSC with differing physicochemical properties. Treatments of the obtained structures with cellulases allow the selective removal of pure cellulose, whereas a TMSC pattern remains on the surface. These TMSC can be regenerated back to pure cellulose by treatments with vapors of hydrochloric acid. The developed methods allow the effective fabrication of micropatterned biopolymer thin films suitable for further functionalization and application in, e.g., bioanalytical devices. This is shown by the immobilization and detection of single‐stranded DNA on structured cellulose surfaces. Owing to the versatility of both patterning approaches the methods can be further extended to other combinations of substrates and enzymes.

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Biotin‐Functionalized Cellulose‐Based Monolayers as Sensitive Interfaces for the Detection of Single Molecules

Cited by 17 publications

References 31 publications

Synthesis and Fluorescent Properties of Carbazole‐Substituted Hydroxyethylcelluloses

Synthesis and Fluorescent Properties of Carbazole‐Substituted Hydroxyethylcelluloses

Membrane surface functionalization via theophylline derivative coating and streptavidin immobilization

Functional Patterning of Biopolymer Thin Films Using Enzymes and Lithographic Methods

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