2017
DOI: 10.3791/56457
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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Abstract: Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lysates and therefore the interactions found will reflect those that occur within the intracellular context. Connexin43 (Cx43), a protein that forms gap junction channe… Show more

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Cited by 2 publications
(3 citation statements)
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“…2b), suggesting altered mitochondrial trafficking. This change in mitochondrial localization might arise from cytoskeleton rearrangement 35 and contribute to the reported TAT-Cx43266-283-induced inhibition of GSC invasion and migration 26 , as previously shown by others 37 .…”
Section: Tat-cx43266-283 Reduces Mitochondrial Metabolism Without Incsupporting
confidence: 58%
See 1 more Smart Citation
“…2b), suggesting altered mitochondrial trafficking. This change in mitochondrial localization might arise from cytoskeleton rearrangement 35 and contribute to the reported TAT-Cx43266-283-induced inhibition of GSC invasion and migration 26 , as previously shown by others 37 .…”
Section: Tat-cx43266-283 Reduces Mitochondrial Metabolism Without Incsupporting
confidence: 58%
“…1d). This might be due to changes in the polymerization state of the actin cytoskeleton caused by TAT-Cx43266-283 35 , which can modulate GLUT-1 clustering without affecting glucose transport 36 . The levels of GLUT-3, another glucose transporter frequently co-opted by cancer cells 13 , were not affected by TAT-Cx43266-283 under these experimental conditions ( Fig.…”
Section: Tat-cx43266-283 Decreases Glucose Uptake Selectively In Gscsmentioning
confidence: 99%
“…Previous studies from our group showed increased glucose uptake in rat astrocytes by the inhibition of Cx43-gap junctional communication [80] through a mechanism involving microtubules, suggesting that changes in the cytoskeleton could participate in the effects of TAT-Cx43 266–283 on glucose uptake. However, TAT-Cx43 266–283 affects actin but not tubulin distribution in GSCs [81] . Furthermore, while the inhibition of Src with dasatinib exerted a similar effect to TAT-Cx43 266–283 on glucose uptake, the disruption of actin polymerisation with latrunculin A did not modify the rate of glucose uptake (data not shown).…”
Section: Discussionmentioning
confidence: 97%