Biotinylation reagents for the study of cell surface proteins

Elia, Giuliano

doi:10.1002/pmic.200800097

Cited by 172 publications

(169 citation statements)

References 64 publications

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“…28,29 Avidin has been widely used for labeling biopolymers and cells because of its strong binding to biotin-tagged molecules, such as fluorophores, DNA, lipids, and proteins. 74 The avidin molecule is roughly cubic (molecular dimensions: ~4.0 × 5.0 × 5.5 nm) and two pairs of biotin binding sites are located on opposite faces of the molecule. 28,29 It is possible to assemble enzyme architectures through the LbL deposition of avidin and biotin-labeled enzymes (Fig.…”

Section: ·2 Biological Affinity-based Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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Section: ·2 Biological Affinity-based Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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“…ubilization Approach-Use of biotin tagging of cell surface proteins together with streptavidin beads has been used to pull down cell surface proteins from cells and tissue (25,26). Thus, we used this approach to enrich plasma membrane proteins from cultured myoblasts.…”

Section: Isolation Of Sarcolemmal Proteome Using the Membrane Sol-mentioning

confidence: 99%

“…At least three different affinity-based enrichment strategies have been developed to increase the purity and yield of cell membrane proteome (22). One approach uses biotin to selectively tag cell surface proteins followed by the use of streptavidin beads to pull down the tagged proteins (25,26). Another approach relies on the assumption that the majority of membrane proteins are glycosylated, and lectin (27) or hydrazide chemistry (28) is used to tag these proteins.…”

mentioning

confidence: 99%

Use of Quantitative Membrane Proteomics Identifies a Novel Role of Mitochondria in Healing Injured Muscles

Sharma

Medikayala

Defour

et al. 2012

Journal of Biological Chemistry

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Background: Cellular processes involved in healing injured skeletal muscle fibers are poorly understood. Results: Using an improved quantitative membrane proteomics approach for cells and tissues, we have identified accumulation of mitochondria at the site of sarcolemma injury as a key requirement for myofiber healing. Conclusion: Mitochondria are the earliest responders to myofiber injury. Significance: This work identifies a novel function of mitochondria in muscle injury.

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“…The availability of func" onal groups, such as carboxyl, amino, thiol and imidazols in protein and enzymes allow interac" on with carbodiimides [22][23][24][25]. The reac" on of carbodiimide alone with a protein can lead to deac" va" on, but since o-acylureas are liable to hydrolysis reac" on occurs in presence of water.…”

Section: Applications Of Carbodiimidesmentioning

confidence: 99%

Polycarbodiimide and polyurethane cross-linkers for leather finishing

Ramkumar¹,

Murali²,

Preethi³

et al. 2017

LFJ

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ABSTRACT. This review focuses on polyurethane and carboxylic acid-carbodiimide cross-linked systems for leather fi nishes. Recently, these cross-links have become very popular in leather fi nishing. The cross-links in leather fi nishes improve the strength proper# es of the fi nished leather, the excess of cross-links causes bri$ leness. The mul# func# onal carbodiimide cross-linkers are useful for leather surface proper# es improvement. The carbodiimide groups are sensi# ve to hydrolysis; life # me of this func# onal group during polymeriza# on is enhanced. Water dispersible isocyanates, polyurethane copolymers and polyisocyanate cross-links have applica# ons in new technologies are discussed in this review. KEY WORDS: cross-linker, polycarbodiimide, diisocyanate, leather, polyurethane AGENŢI DE RETICULARE PE BAZĂ DE POLICARBODIIMIDĂ ŞI POLIEURETAN PENTRU FINISAREA PIELIIREZUMAT. Acest ar# col se concentrează asupra sistemelor de re# culare pe bază de poliuretan şi acid carboxilic-carbodiimidă pentru fi nisarea pielii. Recent, aceste # puri de re# culări au devenit foarte populare în fi nisarea pieilor. Re# cularea pielii la fi nisare îmbunătăţeşte proprietăţile de rezistenţă ale pieii fi nite, în # mp ce excesul de legături încrucişate determină fragilitate. Agenţii de re# culare mul# funcţionali carbodiimidici sunt u# li pentru îmbunătăţirea proprietăţilor de suprafaţă ale pielii. Grupările carbodiimidice sunt sensibile la hidroliză; durata de viaţă a acestui grup funcţional în # mpul polimerizării este sporită. Re# culările cu izocianaţii dispersabili în apă, copolimerii poliuretanici şi poliizocianaţi discutate în această lucrare au aplicaţii în tehnologii noi. CUVINTE CHEIE: agent de re# culare, policarbodiimidă, diizocianat, piele, poliuretan AGENTS DE RÉTICULATION POLYCARBODIIMIDE ET POLYURÉTHANE POUR LA FINITION DU CUIRRÉSUMÉ. Cet ar# cle met l'accent sur les systèmes de ré# cula# on à base de polyuréthane et de l'acide carboxylique-carbodiimide pour la fi ni# on du cuir. Récemment, ces types de ré# cula# ons sont devenus très populaires dans la fi ni# on du cuir. La ré# cula# on du cuir dans l'opéra# on de fi ni# on améliore les propriétés de résistance du cuir fi ni, tandis que la ré# cula# on excessive entraîne une fragilité. Les agents de ré# cula# on mul# fonc# onnels de carbodiimide sont u# les pour améliorer les propriétés de surface du cuir. Les groupes carbodiimide sont sensibles à l'hydrolyse; la durée de vie de ce groupe fonc# onnel pendant la polymérisa# on est augmentée. Les ré# cula# ons avec des isocyanates dispersables dans l'eau, des copolymères de polyuréthane et des polyisocyanates décrits dans cet ar# cle ont des applica# ons dans de nouvelles technologies. MOTS CLÉS: agent de ré# cula# on, polycarbodiimide, diisocyanate, cuir, polyuréthane

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Biotinylation reagents for the study of cell surface proteins

Cited by 172 publications

References 64 publications

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Use of Quantitative Membrane Proteomics Identifies a Novel Role of Mitochondria in Healing Injured Muscles

Polycarbodiimide and polyurethane cross-linkers for leather finishing

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