Biotization of tissue culture banana plantlets with Methylobacterium salsuginis to enhance the survival and growth under greenhouse and open environment condition

Abstract

Aim: This study aimed to investigate the impact of M. salsuginis TNMB03 biotization on tissue culture banana cv. Grande Naine plantlets growth and survival under greenhouse and open environmental condition (exposed to direct sunlight). Methodology: Banana plantlets were transferred from culture flasks to protray and maintained under the greenhouse and open environmental condition for 30 days with or without M. salsuginis TNMB03 treatment. After 30 days, plant growth parameters like pseudostem height, girth, number of leaves, leaf area, fresh and dry biomass, root parameters, plantlet survival, chlorophyll a and b, total chlorophyll, carotenoids and soluble protein and Methylobacterium population in upper and lower surface of leaf, as well as endophytic population were assessed. Results: This study showed that the plantlets biotized with M. salsuginis TNMB03 had better acclimatization response under both the experimental condition than that of uninoculated plantlets. Positive influence on the survival and growth of M. salsuginis TNMB03 biotized plantlets was observed when transferred directly to greenhouse and open environmental condition. Inoculation of M. salsuginis TNMB03 increased the plant height, girth and number of leaves, root length, lateral root and biomass in comparison to the uninoculated plantlets in greenhouse and open environment. Uninoculated plantlets kept under open environment had lower chlorophyll content and sun scorching damages compared to M. salsuginis TNMB03 inoculated plants, which had dark green leaves and increased chlorophyll content. Interpretation: This study shows a new potential technique of using M. salsuginis TNMB03 in tissue culture plantlets, which can help in enhancing the growth of plantlets transferred from culture vessel to greenhouse or open environmental condition without undergoing the routine acclimatization procedure.

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

“…The plantlets are further hardened after their initial adaptation in the plastic tunnel until they reach 25-30 cm in height and have 3-5 leaves in the glasshouse environment [17,19,41]. A significant percentage of in vitro-derived plantlets do not survive when moved from a controlled in vitro environment to an ambient glasshouse or outdoor conditions [39,42], because the juvenile in vitro plantlets are under stress since the glasshouse environment has much lower relative humidity (RH), high irradiation, and septic conditions [22,43,44].…”

Biotization of Endophytes Trichoderma asperellum and Bacillus subtilis in Mentha spicata Microplants to Promote Growth, Pathogen Tolerance and Specialized Plant Metabolites

“…When comparing the treatments with the pathogen (P) and the treatment with the endophytes T. asperellum (T) and with B. subtilis , no significant differences were found ( Figure 5 B). In recent years, research on plant–micro-organism association has been of great scientific and commercial interest due to its effect on improving plant quality, promoting plant development, and tolerance to biotic and abiotic problems [ 17 ].…”

“…which have been widely used as biocontrol agents and biostimulants to inhibit pathogen infection, induce systemic or localized resistance, enhance tolerance to biotic stresses, and improve plant vigor and growth [ 13 , 14 ]. Biotization of microplants is a promising biotechnological practice to improve seedling survival and quality during the acclimatization phase and, under field conditions, increase tolerance to biotic and abiotic stresses; therefore, it can contribute to the reduction of chemical inputs in plant production [ 15 , 16 , 17 ].…”

Impact of Glyphosate and Glyphosate-Based Herbicides on Phyllospheric Methylobacterium

“…Combined with the results presented herein, the relevant literature supports our theory that GBH's may increase crop susceptibility to infections by selectively attenuating the protective effects of Methylobacteria colonization. This is supported by the fact that, in addition to soybean, the genus of Methylobacteria comprises a major part of the phyllosphere in several of the same food crop systems that exhibit increased susceptibility to infectious disease when exposed to GBH's including: apple [130,131], wheat [61, 132,133], tomato [134,135,136], hemp [137], cotton [138,139], and banana [140,141,142,143]. Importantly, the application of glyphosate has also been previously found to decrease host phytoalexin levels, even when attempting to elicit an immune response through deliberate pathogen challenge [144,145].…”