Biotoxin Detection Using Cell-Based Sensors

Banerjee, Pratik; Kintzios, Spyridon; Prabhakarpandian, Balabhaskar

doi:10.3390/toxins5122366

Cited by 52 publications

(27 citation statements)

References 97 publications

(168 reference statements)

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“…Therefore, to tackle this limitation, based on microbial whole-cell arrays principle, a wide spectrum of cell-based biosensors with an added value of living cells as a sensing element have been developed for various applications in different bio-and non-bio sectors of the modern world. Figure 2 shows a generalized working principle of cellular sensors [28]. Rider et al [29] developed a genetically engineered cell lines-based sensor for a rapid identification of pathogens using membrane incorporated pathogen-specific antibodies.…”

Section: Microbial Whole-cell Arraysmentioning

confidence: 99%

Advancements and Potential Applications of Microfluidic Approaches—A Review

Ahmed¹,

Akram²,

Bule

et al. 2018

Chemosensors

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A micro-level technique so-called “microfluidic technology or simply microfluidic” has gained a special place as a powerful tool in bioengineering and biomedical engineering research due to its core advantages in modern science and engineering. Microfluidic technology has played a substantial role in numerous applications with special reference to bioscience, biomedical and biotechnological research. It has facilitated noteworthy development in various sectors of bio-research and upsurges the efficacy of research at the molecular level, in recent years. Microfluidic technology can manipulate sample volumes with precise control outside cellular microenvironment, at micro-level. Thus, enable the reduction of discrepancies between in vivo and in vitro environments and reduce the overall reaction time and cost. In this review, we discuss various integrations of microfluidic technologies into biotechnology and its paradigmatic significance in bio-research, supporting mechanical and chemical in vitro cellular microenvironment. Furthermore, specific innovations related to the application of microfluidics to advance microbial life, solitary and co-cultures along with a multiple-type cell culturing, cellular communications, cellular interactions, and population dynamics are also discussed.

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Section: Microbial Whole-cell Arraysmentioning

confidence: 99%

Advancements and Potential Applications of Microfluidic Approaches—A Review

Ahmed¹,

Akram²,

Bule

et al. 2018

Chemosensors

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“…A SPR biosensor 183 has been developed for detection of three botulinum neurotoxin serotypes (A, B, and F) with detection limits between 0.5 and 1 ng/mL in less than an hour. CBBs are emerging as an alternative 18 to ELISA and immuno-PCR methods by eliminating shortcomings such as the inability of ELISA to differentiate between active or inactive toxins or holotoxin and reduced toxin. Human-induced pluripotent stem cells (hiPSCs) have been applied for detecting botulinum neurotoxin and achieving the quantitative determination of different serotypes.…”

Section: Biological Warfare Agentsmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

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The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for onsite diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.

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“…Cell-based sensors have been developed for PSP toxins, which utilize only cells instead of animals [11,12,13]. In 2012, Van Dolan et al described a receptor-based assay that was able to detect PSP toxins near the regulatory limits [4,14].…”

Section: Introductionmentioning

confidence: 99%

Integrating scFv into xMAP Assays for the Detection of Marine Toxins

Shriver‐Lake

Liu

Lee

et al. 2016

Toxins

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Marine toxins, such as saxitoxin and domoic acid are associated with algae blooms and can bioaccumulate in shell fish which present both health and economic concerns. The ability to detect the presence of toxin is paramount for the administration of the correct supportive care in case of intoxication; environmental monitoring to detect the presence of toxin is also important for prevention of intoxication. Immunoassays are one tool that has successfully been applied to the detection of marine toxins. Herein, we had the variable regions of two saxitoxin binding monoclonal antibodies sequenced and used the information to produce recombinant constructs that consist of linked heavy and light variable domains that make up the binding domains of the antibodies (scFv). Recombinantly produced binding elements such as scFv provide an alternative to traditional antibodies and serve to “preserve” monoclonal antibodies as they can be easily recreated from their sequence data. In this paper, we combined the anti-saxitoxin scFv developed here with a previously developed anti-domoic acid scFv and demonstrated their utility in a microsphere-based competitive immunoassay format. In addition to detection in buffer, we demonstrated equivalent sensitivity in oyster and scallop matrices. The potential for multiplexed detection using scFvs in this immunoassay format is demonstrated.

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Biotoxin Detection Using Cell-Based Sensors

Cited by 52 publications

References 97 publications

Advancements and Potential Applications of Microfluidic Approaches—A Review

Advancements and Potential Applications of Microfluidic Approaches—A Review

Development and Applications of Portable Biosensors

Integrating scFv into xMAP Assays for the Detection of Marine Toxins

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