Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

Wang, Qiaoning; Wang, Hongdan; Jiang, Yaru; Lv, Min; Wang, Xiaoyan; Chen, Lingxin

doi:10.1016/j.jhazmat.2020.125023

Cited by 16 publications

(2 citation statements)

References 84 publications

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“…The excessive abuse and uncontrolled discharges of antibiotics pose a severe threat to ecological safety and public health. Sulfamethoxazole (SMX), classified as one of the top 10 priority drugs in European countries, has been frequently detected in various water bodies with an average concentration of 0.01–2.0 μg/L. , Unfortunately, long-term exposure to low doses of SMX can induce mass reproduction of antibiotic-resistant bacteria and transmission resistance genes. , Thus, it is urgent to develop an effective technology for removing SMX from natural water bodies.…”

Section: Introductionmentioning

confidence: 99%

MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

et al. 2022

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By anchoring MoS2 nanosheets onto FeS2 derived from different MIL-100(Fe) precursors, a series of FeS2@MoS2-x samples featuring sulfur vacancies (SVs) were prepared as efficient peroxymonosulfate (PMS) activators to degrade sulfamethoxazole (SMX) from aqueous solution. Benefiting from the strongly reductive sulfur species (S2– and S2 2–), enriched Mo(IV) sites, and abundant SVs, 40 μM SMX was completely removed by the FeS2@MoS2-2/PMS system in 7 min (0.2 g/L FeS2@MoS2-2, 0.25 mM PMS). The k obs obtained by FeS2@MoS2-2 was 0.598 min–1, which was 5.8 and 51.1 times higher than that of FeS2 (0.103 min–1) and MoS2 (0.012 min–1), respectively. Quenching experiments, electron paramagnetic resonance (EPR) analysis, and 18O isotope labeling tests evidenced the involvement of radical (•OH, SO4 •–) and non-radical (1O2, FeIV = O) pathways in the FeS2@MoS2-2/PMS system, and MoS2 anchoring enormously enhanced the contribution of non-radicals to 45.5%. In addition, SVs possessed favorable affinity toward PMS and dissolved oxygen (DO), promoting continuous production of reactive active species. The degradation pathways of SMX were unveiled as well. The satisfactory recyclability, stability, and universality enabled FeS2@MoS2-2 to serve as a promising candidate for PMS activation. This study provides a novel strategy to construct sulfur vacancy-featuring Fe-based sulfide catalysts using MIL-100(Fe) as sacrificial templates for activating PMS to treat refractory organic-polluted water.

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Section: Introductionmentioning

confidence: 99%

MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

et al. 2022

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“…The samples from control were defined as CK-1, CK-2, and CK-3, while samples from selenite treatment were set as Se-1, Se-2, and Se-3, respectively. The total RNA extraction, RNA integrity assessment, and cDNA library construction were conducted at Beijing Genomics Institute (BGI, Shenzhen, China) according to the methods described by Wang et al (2021) . Finally, RNA- sequencing of the cDNA library was carried out on a BGISEQ-500 platform (BGI, Shenzhen, China).…”

Section: Methodsmentioning

confidence: 99%

Selenite Reduction by Proteus sp. YS02: New Insights Revealed by Comparative Transcriptomics and Antibacterial Effectiveness of the Biogenic Se0 Nanoparticles

Wang

Sun

et al. 2022

Front. Microbiol.

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Biotransformation of selenite by microorganisms is an effective detoxification (in cases of dissimilatory reduction, e.g., to Se0) and assimilation process (when Se is assimilated by cells). However, the current knowledge of the molecular mechanism of selenite reduction remains limited. In this study, a selenite-resistant bacterium was isolated and identified as Proteus sp. YS02. Strain YS02 reduced 93.2% of 5.0 mM selenite to selenium nanoparticles (SeNPs) within 24 h, and the produced SeNPs were spherical and localized intracellularly or extracellularly, with an average dimension of 140 ± 43 nm. The morphology and composition of the isolated and purified SeNPs were characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM) with energy-dispersive X-ray (EDX) spectrometry, and Fourier transform infrared (FTIR) spectroscopy. FTIR spectroscopy indicated the presence of proteins, polysaccharides, and lipids on the surface of the isolated SeNPs. Furthermore, the SeNPs showed excellent antimicrobial activity against several Gram-positive and Gram-negative pathogenic bacteria. Comparative transcriptome analysis was performed to elucidate the selenite reduction mechanism and biosynthesis of SeNPs. It is revealed that 197 genes were significantly upregulated, and 276 genes were significantly downregulated under selenite treatment. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that genes associated with ABC transporters, sulfur metabolism, pentose phosphate pathway (PPP), and pyruvate dehydrogenase were significantly enhanced, indicating selenite is reduced by sulfite reductase with PPP and pyruvate dehydrogenase supplying reducing equivalents and energy. This work suggests numerous genes are involved in the response to selenite stress, providing new insights into the molecular mechanisms of selenite bioreduction with the formation of SeNPs.

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Biological transformation as a technique in pollution decontamination

Nouri

2022

Microbial Consortium and Biotransformation for Pollution Decontamination

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Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

Cited by 16 publications

References 84 publications

MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

Selenite Reduction by Proteus sp. YS02: New Insights Revealed by Comparative Transcriptomics and Antibacterial Effectiveness of the Biogenic Se0 Nanoparticles

Biological transformation as a technique in pollution decontamination

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Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

Cited by 16 publications

References 84 publications

MoS2 Nanosheets Anchored onto MIL-100(Fe)-Derived FeS2 as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

MoS2 Nanosheets Anchored onto MIL-100(Fe)-Derived FeS2 as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

Selenite Reduction by Proteus sp. YS02: New Insights Revealed by Comparative Transcriptomics and Antibacterial Effectiveness of the Biogenic Se0 Nanoparticles

Biological transformation as a technique in pollution decontamination

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MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism

MoS₂ Nanosheets Anchored onto MIL-100(Fe)-Derived FeS₂ as a Peroxymonosulfate Activator for Efficient Sulfamethoxazole Degradation: Insights into the Mechanism