Biotransformation of 4-methylcoumarins by cambial meristematic cells of Camptotheca acuminata

Zhang, Yuhua; Jiang, Jia-Yi; Qin, Ningbo; Zhang, Qian; Yan, Chunyan

doi:10.1039/c9ra00522f

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…The cultures of stem cell lines with a very high proportion of stem cells were established in this way. Until now, stem cell cultures have been established exclusively from isolated and cultured innately undifferentiated cambial meristematic cells from stem segments [22,[29][30][31][32][33]. However, the use of cambial meristematic cells from stem explants may not be the only way to establish an in vitro culture of stem cells.…”

Section: Discussionmentioning

confidence: 99%

Suspension Culture of Stem Cells Established from Calendula officinalis L.

Kaňuková¹,

Lenkavská²,

Gubišová³

et al. 2023

Preprint

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Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allows to consider the establishment of stem cell cultures in a broader range of species. Therefore, this study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from explants taken from leaves and roots. Appropriate combinations of plant growth regulators and light parameters for in vitro cultivation were selected. Already at this stage, stem and dedifferentiated cells could be identified, and cell suspension cultures were established from specific parts of the formed callus. Cell suspension cultures with a high proportion of stem cells originating from roots (92–93%) or leaves (72–73%) were developed. The amount of stem cells derived from the roots in the cell suspension reached a plateau at a level of 5.60–5.72105.

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Section: Discussionmentioning

confidence: 99%

Suspension Culture of Stem Cells Established from Calendula officinalis L.

Kaňuková¹,

Lenkavská²,

Gubišová³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…To date, several studies on CMCs have been reported, but only in a limited number of plant species, mainly medicinal ones, e.g., Taxus cuspidata , Ginkgo biloba , Solanum lycopersicon 22 , Panax ginseng 22 , 28 , Catharanthus roseus 29 , Tripterygium wilfordii 30 , Camptotheca acuminate 31 , Ocimum basilicum 32 , Fraxinus mandshurica 33 . However, no study has been presented on the establishment and cultivation of stem cell or stem-like cell suspension cultures derived from Calendula officinalis L. Establishment of stem cell cultures in small volumes or in bioreactors should ensure higher production of cells and secondary metabolites in comparison with the cultivation of dedifferentiated cells in suspension cultures.…”

Section: Introductionmentioning

confidence: 99%

Suspension culture of stem cells established of Calendula officinalis L.

Kaňuková,

Lenkavská,

Gubišová

et al. 2024

Sci Rep

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Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allow to consider the establishment of stem cell cultures in a broader range of species. This study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from leaf and root explants, and already at this stage, stem and dedifferentiated cells could be identified. The cell suspension cultures established both from the root- and leaf-derived calli contained a high proportion of stem cells (92–93% and 72–73%, respectively). The most effective combination of growth regulators for the development of stem cells in calli as well as cell cultures was 1.0 mg/L 2,4-D and 0.5 mg/L BAP. The highest amount of stem cells (5.60–5.72 × 105) was in cell suspension derived from the roots. An effective protocol for the establishment of marigold stem cell suspension culture was developed. The ratio of root-derived stem cells against dedifferentiated cells exceeded 90%.

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Biotransformation of 4-methylcoumarins by cambial meristematic cells of Camptotheca acuminata

Abstract: Cambial meristematic cell (CMC) suspension cultures were investigated as a new biotransformation system for the first time.

Cited by 2 publications

References 33 publications

Suspension Culture of Stem Cells Established from <em>Calendula officinalis </em>L.

Suspension Culture of Stem Cells Established from <em>Calendula officinalis </em>L.

Suspension culture of stem cells established of Calendula officinalis L.

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