Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia

Sherwood, Juli L.; Petersen, James N.; Skeen, Rodney S.

doi:10.1002/(sici)1097-0290(19990805)64:3<342::aid-bit10>3.0.co;2-4

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…With benzoate as the primary substrate, the presence of nitrate resulted in less extensive CT transformations than without nitrate, consistent with the findings of Semprini et al (1992), Stensel and DeJong (1994), Petersen et al (1994), and Sherwood et al (1996Sherwood et al ( , 1999. However, when acetate was the primary substrate, equal or slightly greater CT transformation was observed in the presence of nitrate.…”

Section: Discussionsupporting

confidence: 86%

“…Nitrate reduction has particular appeal in this case due to significant nitrate contamination in Hanford ground water (Table 1). Interestingly, the results of Sherwood et al (1996, 1999) showed that relatively greater rates of CT transformation were achieved under nitrate‐limiting conditions. CT transformation continued in the absence of a primary substrate, although at a lesser rate, with less CF production on a relative basis.…”

Section: Introductionmentioning

confidence: 99%

“…However, the potential of this technology to succeed at the Hanford site remains in question. Of primary concern is the production of chloroform (CF, CHCl 3 ) as a toxic by-product of the biotransformation process by the microbial consortia indigenous to Hanford (Sherwood et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material

Niemet¹,

Semprini²

2005

Groundwater Monitoring Rem

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Continuous-flow and batch experiments were conducted using a column reactor system containing Hanford Aquifer material in order to assess the potential of in situ bioremediation of carbon tetrachloride (CT) at the Hanford site in southcentral Washington state. Benzoate and acetate were evaluated as primary substrates both in the presence and absence of nitrate as a potential electron acceptor. Each of the four resulting test conditions was first run under continuous-flow mode until a pseudo-steady state was achieved and then switched to batch mode. The longer residence time of the batch portion of the test resulted in more complete transformations and helped elucidate zones of variable activity within the column. Reductions in CT concentration and chloroform (CF) production were observed in all test conditions. Benzoate generally supported faster and more complete CT transformations than acetate, even though substrate use and denitrification was more rapid for acetate. Sulfate was present in all cases; yet, sulfate reduction was never observed, even during extended absences of nitrate and nitrite. CT transformation was most rapid near the point of injection, with rates decreasing toward the effluent end of the column. These results indicate that the microbial population at Hanford is capable of transforming CT in the subsurface. However, methods to control the production of CF may be necessary before this technology can be successfully implemented in the field.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material

Niemet¹,

Semprini²

2005

Groundwater Monitoring Rem

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Bioremediation: An Overview of How Microbiological Processes can be Applied to the Cleanup of Organic and Inorganic Environmental Pollutants

Prince

2003

Encyclopedia of Environmental Microbiology

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Bioremediation, Ecology, Technology Organic Contaminants Halogenated Aliphatic Compounds Halogenated Aromatic Compounds Nonchlorinated Pesticides and Herbicides Military Chemicals Other Organic Compounds Inorganic Contaminants Metals and Metalloids

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Microbial degradation of tetrachloromethane: mechanisms and perspectives for bioremediation

Penny¹,

Vuilleumier²,

Bringel³

2010

FEMS Microbiology Ecology

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Toxic man-made compounds released into the environment represent potential nutrients for bacteria, and microorganisms growing with such compounds as carbon and energy sources can be used to clean up polluted sites. However, in some instances, microorganisms contribute to contaminant degradation without any apparent benefit for themselves. Such cometabolism plays an important part in bioremediation, but is often difficult to control. Microbial degradation of tetrachloromethane (carbon tetrachloride, CCl(4)), a toxic ozone-depleting organic solvent mainly of anthropogenic origin, is only known to occur by cometabolic reduction under anoxic conditions. Yet no microbial system capable of using CCl(4) as the sole carbon source has been described. Microbial growth based on CCl(4) as a terminal electron acceptor has not been reported, although corresponding degradation pathways would yield sufficient energy. Known modes for the biodegradation of CCl(4) involve several microbial metabolites, mainly metal-bound coenzymes and siderophores, which are produced by facultative or strictly anaerobic bacteria and methanogenic Archaea. Recent reports have demonstrated that CCl(4) dechlorination rates are enhanced by redox-active organic compounds such as humic acids and quinones, which act as shuttles between electron-providing microorganisms and CCl(4) as a strong electron acceptor. The key factors underlying dechlorination of CCl(4), the practical aspects and specific requirements for microorganism-associated degradation of CCl(4) at contaminated sites and perspectives for future developments are discussed.

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Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia

Cited by 3 publications

References 21 publications

Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material

Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material

Bioremediation: An Overview of How Microbiological Processes can be Applied to the Cleanup of Organic and Inorganic Environmental Pollutants

Microbial degradation of tetrachloromethane: mechanisms and perspectives for bioremediation

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