Biotransformation of chloramphenicol by white-rot-fungi Trametes versicolor under cadmium stress

Tan, Zewen; Losantos, Diana; Li, Yongtao; Sarrà, Montserrat

doi:10.1016/j.biortech.2022.128508

Cited by 14 publications

(2 citation statements)

References 48 publications

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“…In evaluating the efficacy of the WRF for removing TnBP and TBEP, both G. lucidum and T. versicolor demonstrated remarkable efficiency, by removing more than 90% of these compounds within a very short period (4 days). This proficiency is consistent with findings from other studies where this ability by the fungi to remove complex xenobiotics was tested (Beltrań-Flores et al, 2022;Shokrollahzadeh et al, 2023;Tan et al, 2023). Although exhibiting lower overall performances, P. ostreatus and P. sanguineus are also strong candidates for the removal of these OPFRs.…”

Section: Fungal Selection For Opfr Removalsupporting

confidence: 91%

“…The main objective of this work was to identify fungal candidates capable of effectively removing water-soluble OPFRs, while using organic matter present in wastewater. The screening included four WRF, well-studied by the group (Palli et al, 2017;Jaeń-Gil et al, 2019;Beltrań-Flores et al, 2021;Tan et al, 2023), but also two Ascomycota fungi. These Ascomycota fungi, selected from the Trichoderma and Aspergillus genera, also produce extracellular ligninolytic enzymes (laccase and peroxidases) (Dhakar et al, 2015;Kurkina et al, 2021), have demonstrated high efficiency in removing contaminants (Tripathi et al, 2013;Mukherjee, 2016), and possess a range of other enzymes such as oxidases, cellulases, xylanases, and amylases, which could potentially contribute to the degradation of either the OPFRs or other complex constituents in wastewater.…”

Section: Introductionmentioning

confidence: 99%

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OPFR removal by white rot fungi: screening of removers and approach to the removal mechanism

Losantos,

Sarra,

Caminal

2024

Front. Fungal Biol.

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The persistent presence of organophosphate flame retardants (OPFRs) in wastewater (WW) effluents raises significant environmental and health concerns, highlighting the limitations of conventional treatments for their remotion. Fungi, especially white rot fungi (WRF), offer a promising alternative for OPFR removal. This study sought to identify fungal candidates (from a selection of four WRF and two Ascomycota fungi) capable of effectively removing five frequently detected OPFRs in WW: tributyl phosphate (TnBP), tributoxy ethyl phosphate (TBEP), trichloroethyl phosphate (TCEP), trichloro propyl phosphate (TCPP) and triethyl phosphate (TEP). The objective was to develop a co-culture approach for WW treatment, while also addressing the utilization of less assimilable carbon sources present in WW. Research was conducted on carbon source uptake and OPFR removal by all fungal candidates, while the top degraders were analyzed for biomass sorption contribution. Additionally, the enzymatic systems involved in OPFR degradation were identified, along with toxicity of samples after fungal contact. Acetate (1.4 g·L-1), simulating less assimilable organic matter in the carbon source uptake study, was eliminated by all tested fungi in 4 days. However, during the initial screening where the removal of four OPFRs (excluding TCPP) was tested, WRF outperformed Ascomycota fungi. Ganoderma lucidum and Trametes versicolor removed over 90% of TnBP and TBEP within 4 days, with Pleorotus ostreatus and Pycnoporus sanguineus also displaying effective removal. TCEP removal was challenging, with only G. lucidum achieving partial removal (47%). A subsequent screening with selected WRF and the addition of TCPP revealed TCPP’s greater susceptibility to degradation compared to TCEP, with T. versicolor exhibiting the highest removal efficiency (77%). This observation, plus the poor degradation of TEP by all fungal candidates suggests that polarity of an OPFR inversely correlates with its susceptibility to fungal degradation. Sorption studies confirmed the ability of top-performing fungi of each selected OPFR to predominantly degrade them. Enzymatic system tests identified the CYP450 intracellular system responsible for OPFR degradation, so reactions of hydroxylation, dealkylation and dehalogenation are possibly involved in the degradation pathway. Finally, toxicity tests revealed transformation products obtained by fungal degradation to be more toxic than the parent compounds, emphasizing the need to identify them and their toxicity contributions. Overall, this study provides valuable insights into OPFR degradation by WRF, with implications for future WW treatment using mixed consortia, emphasizing the importance of reducing generated toxicity.

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Section: Fungal Selection For Opfr Removalsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

OPFR removal by white rot fungi: screening of removers and approach to the removal mechanism

Losantos,

Sarra,

Caminal

2024

Front. Fungal Biol.

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Novel method for rapid monitoring of OPFRs by LLE and GC–MS as a tool for assessing biodegradation: validation and applicability

Losantos,

Palacios,

Berge

et al. 2024

Anal Bioanal Chem

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Organophosphate flame retardants (OPFRs) are high-production volume chemicals widely present in environmental compartments. The presence of water-soluble OPFRs (tri-n-butyl phosphate (TnBP), tris(2-butoxyethyl) phosphate (TBEP), tris(2-chloroethyl) phosphate (TCEP), tris(2-chloroisopropyl) phosphate (TCPP), and triethyl phosphate (TEP)) in water compartments evidences the struggle of conventional wastewater treatment plants (WWTPs) to effectively eliminate these toxic compounds. This study reports for the first time the use of white-rot fungi as a promising alternative for the removal of these OPFRs. To accomplish this, a simple and cost-efficient quantification method for rapid monitoring of these contaminants’ concentrations by GC–MS while accounting for matrix effects was developed. The method proved to be valid and reliable for all the tested parameters. Sample stability was examined under various storage conditions, showing the original samples to be stable after 60 days of freezing, while post-extraction storage techniques were also effective. Finally, a screening of fungal degraders while assessing the influence of the glucose regime on OPFR removal was performed. Longer chain organophosphate flame retardants, TBP and TBEP, could be easily and completely removed by the fungus Ganoderma lucidum after only 4 days. This fungus also stood out as the sole organism capable of partially degrading TCEP (35% removal). The other chlorinated compound, TCPP, was more easily degraded and 70% of its main isomer was removed by T. versicolor. However, chlorinated compounds were only partially degraded under nutrient-limiting conditions. TEP was either not degraded or poorly degraded, and it is likely that it is a transformation product from another OPFR’s degradation. These results suggest that degradation of chlorinated compounds is dependent on the concentration of the main carbon source and that more polar OPFRs are less susceptible to degradation, given that they are less accessible to radical removal by fungi. Overall, the findings of the present study pave the way for further planned research and a potential application for the degradation of these contaminants in real wastewaters. Graphical Abstract

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Biotransformation of enrofloxacin-copper combined pollutant in aqueous environments by fungus Cladosporium cladosporioides (CGMCC 40504)

Ma,

Zhang,

Yuan

et al. 2024

World J Microbiol Biotechnol

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Biotransformation of chloramphenicol by white-rot-fungi Trametes versicolor under cadmium stress

Cited by 14 publications

References 48 publications

OPFR removal by white rot fungi: screening of removers and approach to the removal mechanism

OPFR removal by white rot fungi: screening of removers and approach to the removal mechanism

Novel method for rapid monitoring of OPFRs by LLE and GC–MS as a tool for assessing biodegradation: validation and applicability

Biotransformation of enrofloxacin-copper combined pollutant in aqueous environments by fungus Cladosporium cladosporioides (CGMCC 40504)

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