Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil

Abstract

Chlorothalonil is a widely used fungicide, but the contamination of soil and water environments by this chemical causes potential threats to biodiversity. Given the metabolic potential of soil microorganisms, there is a need for developing microbiological approaches to degrade persistent compounds, such as chlorothalonil, in contaminated sites. Here in this study, we isolated a bacterial strain (namely, BJ1) capable of degrading chlorothalonil from a chlorothalonil-contaminated farmland soil in the Shandong Province, China. Using 16S rDNA gene sequencing, morphological and biological characteristics, we identified the strain BJ1 as Stenotrophomonas acidaminiphila. The strain BJ1 uses chlorothalonil as a sole carbon source. At initial concentrations of 50, 100, 200 and 300 mg l−1, it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30°C and pH 7.0). Two metabolites, methyl-2,5,6-trichloro-3-cyano-4-methoxy-benzoate and methyl-3-cyano-2,4,5,6-tetrachlorobenzoate, were detected and identified based on HPLC–MS analysis, which suggests that the strain BJ1 metabolized chlorothalonil through the hydroxylation of chloro-group and hydration of cyano-group. The results of this study highlight the great potential for this bacterium to be used in chlorothalonil pollution remediation.

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

“…Stenotrophomonas sp. was previously reported to degrade organophosphorus pesticides [ 49 ], chlorothalonil [ 50 ], butachlor [ 51 ], and aflatoxin B1 [ 52 ]. To our knowledge, this is the first research reporting on DBP degradation by S. acidaminiphila .…”

“…The initial enrichment culture flask was incubated at 30°C and 180 rpm for 5 days. Subsequently, 5 ml medium aliquots were transferred to new Erlenmeyer flasks containing 50 ml LB/MM medium and different DBP concentrations (50,100,200, 400, 800, 1,600 mg/l). After six rounds of culturing, the final suspensions were diluted, plated, and incubated on MM agar plates containing 50 mg/l DBP [1] to obtain a single colony using DBP as the sole carbon and energy source.…”

Biodegradation of cefalexin by two bacteria strains from sewage sludge

“…The optical density values at a wavelength of 600 nm (OD 600 ) were tested at the same interval as cefalexin concentration with a UV-2365 ultraviolet spectrophotometer (Unicosh Inc., Shanghai, China) for measurement of bacterial growth according to the method established by previous studies [25].…”

Aislamiento, identificación y caracterización de cepas bacterianas con potencial de degradación de los plaguicidas clorotalonil y clorpirifos

“…No obstante, existen innumerables informes de especies bacterianas capaces de tolerar y degradar este tipo de plaguicidas; por ejemplo, Enterobacter cloacae TUAH-1 y Stenotrophomonas acidaminiphila BJ1 (Zhang, et al, 2019) pueden metabolizar clorotalonil, mientras que otras, como Pseudomonas aeruginosa, o Bacillus cereus (Lakshmi, Kumar & Khanna, 2008) y Alcaligenes faecalis (Yang, et al, 2005) pueden degradar clorpirifos.…”