Biotransformation of Eugenol via Protocatechuic Acid by Thermophilic Geobacillus sp. AY 946034 Strain

Giedraityte, G.B.; Kalėdienė, Lilija

doi:10.4014/jmb.1309.09069

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…The Geobacillus sp. was capable to grow with eugenol 0.01% (v/v) as the carbon resource and enhanced bacterial growth about eight times 33 .…”

Section: Discussionmentioning

confidence: 99%

“…It is showed that honeybees affect oral microbiome composition 24 . P. uorescens, P. putida, Corynebacterium sp., Fusarium solani, Byssochlamysfulva, and Penicillium simplicissimum, can degrade eugenol, an antibacterial agent present in clove 33 . A few eugenolconverting enzymes have been described, including vanillyl alcohol oxidase from Penicillium simplicissimum 34 , vanillin dehydrogenase from Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

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Cross pollination and inflorescence branching in the presence of Mycoacterium canetti in Floral microbiome of Syzygium aromaticum (L) Merr. and Perry

Santhakumari,

Sarojini

2024

Preprint

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Background Plant associated microorganisms have role in growth and development of plant, prevent infestation by plant pathogens. Purticularly, floral biome influence nectar taste and scent chemistry, by which insects are attracted to visit flowers. Methyl p-anisate and methyl phenylacetate (jasmine-like odour) produced by mycobacteria present in floral parts of several plants moreover honeybees are able to sense the presence of Mycobacterium tuberculosis. Genetic variants exists in highly self-pollinated Syzygium aromaticum which may occur only by cross pollination. Results A less nectar producing clove flower buds may be cross pollinated by the help of microbiome which has a Mycoacterium canetti as an abundant member. In the selected cultivars branching of inflorescence were observed may be due to as a consequence plant hormone elicited by the mycobacterium. Conclusions Mycoacterium canetti may help in cross pollination of plant and therby hereby helping genome diversity Keywords Syzygium aromaticum, Mycobacterium canetti, Floral biome, Pollination, genetic diversity

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“…The Geobacillus sp. was capable to grow with eugenol 0.01% (v/v) as the carbon resource and enhanced bacterial growth about eight times 33 .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cross pollination and inflorescence branching in the presence of Mycoacterium canetti in Floral microbiome of Syzygium aromaticum (L) Merr. and Perry

Santhakumari,

Sarojini

2024

Preprint

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“…To fulfill the huge market demand for flavor and fragrance, the relevant industries rely mostly on chemical methods of synthesis. Presently, various chemically synthesized flavors are available at cheap prices that lead to the generation of various harmful by‐products . So, taking into consideration the consumer's health interest, trends are shifting towards the use of green, eco‐friendly, and healthy processes for the generation of natural products.…”

Section: Introductionmentioning

confidence: 99%

“…prices that lead to the generation of various harmful byproducts [2]. So, taking into consideration the consumer's health interest, trends are shifting towards the use of green, eco-friendly, and healthy processes for the generation of natural products.…”

Section: Introductionmentioning

confidence: 99%

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

Singh

Sachan

Mukhopadhyay

et al. 2020

J of Separation Science

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An efficient ultra high performance liquid chromatography method of separation was developed for the analysis of six important methoxyphenol derivatives involved in the eugenol catabolic pathway. In the present study, an Acquity UPLC BEH C18 column was used for the chromatographic separation of the industrially important phenolic compounds such as vanillin, vanillic acid, ferulic acid, coniferyl alcohol, and coniferyl aldehyde obtained during microbial transformation of eugenol. Eluted components were identified using the dual wavelength (254 and 310 nm) UV detector. A gradient method of elution using mobile phase of aqueous 1 mM trifluoroacetic acid (Solvent A) and methanol (Solvent B) at a flow rate of 0.3 mL/min separated all the five intermediate methoxyphenol derivatives along with their precursor eugenol within 15 min with stable baseline resolution. Method validation was performed for the accurate quantification of vanillin, coniferyl aldehyde, and eugenol using the parameters of linearity, specificity, precision, limit of detection, limit of quantification, and robustness. The developed method would be helpful for clear separation and identification of the five most important intermediate metabolites of the eugenol catabolism pathway.

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“…Although mostly reported from mesophilic bacteria, a few thermophilic microorganisms have also been found to possess lignoaromatic-degrading potential (23)(24)(25)(26). Interestingly, immobilized protocatechuate 3,4-dioxygenase from Pseudomonas putida and dehydrogenases (DesV and LigV) from Sphingobium sp.…”

mentioning

confidence: 99%

A Novel Gene Cluster Is Involved in the Degradation of Lignin-Derived Monoaromatics in Thermus oshimai JL-2

Chakraborty

Suzuki-Minakuchi

Tanaka

et al. 2021

Appl Environ Microbiol

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A novel gene cluster involved in the degradation of lignin-derived monoaromatics such as p-hydroxybenzoate, vanillate, and ferulate has been identified in the thermophilic nitrate reducer Thermus oshimai JL-2. Based on conserved domain analyses and metabolic pathway mapping, the cluster was classified into upper and peripheral pathway operons. The upper pathway genes, responsible for the degradation of p-hydroxybenzoate and vanillate, are located on a 0.27 Mb plasmid, whereas the peripheral pathway genes, responsible for the transformation of ferulate, are spread throughout the plasmid and the chromosome. In addition, a lower pathway operon was also identified in the plasmid that corresponds to the meta-cleavage pathway of catechol. Spectrophotometric and gene induction data suggest that the upper and lower operons are induced by p-hydroxybenzoate, which the strain can degrade completely within 4 days of incubation, whereas the peripheral genes are expressed constitutively. The upper degradation pathway follows a less common route, proceeding via the decarboxylation of protocatechuate to form catechol, and involves a novel thermostable γ-carboxymuconolactone decarboxylase homolog, identified as protocatechuate decarboxylase based on gene deletion experiments. This gene cluster is conserved in only a few Thermales and shows traces of vertical expansion of catabolic pathways in these organisms toward lignoaromatics. IMPORTANCE High-temperature steam treatment of lignocellulosic biomass during the extraction of cellulose and hemi-cellulose fractions leads to the release of a wide array of lignin-derived aromatics into the natural ecosystem, some of which can have detrimental effects on the environment. Not only will identifying organisms capable of using such aromatics aid in environmental cleanup, but thermostable enzymes, if characterized, can be used for efficient lignin valorization. However, no thermophilic lignin degraders have been reported thus far. The present study reports T. oshimai JL-2 as a thermophilic bacterium with the potential to use lignin-derived aromatics. The identification of a novel thermostable protocatechuate decarboxylase gene in the strain further adds to its significance, as such an enzyme can be efficiently used in the biosynthesis of cis,cis-muconate, an important intermediate in the commercial production of plastics.

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Biotransformation of Eugenol via Protocatechuic Acid by Thermophilic Geobacillus sp. AY 946034 Strain

Cited by 9 publications

References 33 publications

Cross pollination and inflorescence branching in the presence of Mycoacterium canetti in Floral microbiome of Syzygium aromaticum (L) Merr. and Perry

Cross pollination and inflorescence branching in the presence of Mycoacterium canetti in Floral microbiome of Syzygium aromaticum (L) Merr. and Perry

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

A Novel Gene Cluster Is Involved in the Degradation of Lignin-Derived Monoaromatics in Thermus oshimai JL-2

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