Biotransformation of lactose to galactitol

Muniruzzaman, Syed; Itoh, Hiromichi; Yoshino, Akira; Katayama, Takuya; Izumori, Ken

doi:10.1016/0922-338x(94)90204-6

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…Galactitol has been produced using chemical routes based on hydrogenation [11]. It has also been reported that is produced by several yeast during growth on galactose [48, 49]. In one notable study, thirteen yeasts were screened for galactitol production using galactose as a sole carbon source [49].…”

Section: Discussionmentioning

confidence: 99%

“…However, the key advantage of using R. toruloides is that it can also co-produce other value-added chemicals, in particular lipids and lipid-based chemicals. In addition to yeast, the bacterium Mycobacterium smegmatis was found to produce 7 g/L galactitol from 10 g/L galactose and 10 g/L glucose [48]. This appears to be the only reported case of galactitol production in bacteria.…”

Section: Discussionmentioning

confidence: 99%

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Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Jagtap

Bedekar

Liu

et al. 2019

Biotechnol Biofuels

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Background: Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Results: Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Conclusions: Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymers with applications in medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloides can produce multiple value-added chemicals from a wide range of sugars.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Jagtap

Bedekar

Liu

et al. 2019

Biotechnol Biofuels

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“…Muniruzzaman et al constructed a new method for galactitol production from lactose. In this method, lactose was first transformed into galactose by commercial β-D-galactosidase, subsequently converted to galactitol by Mycobacterium smegmatis SMDU strains (Muniruzzaman et al 1994). Using the engineered Saccharomyces cerevisiae, 3.5 g/L galactitol was obtained from 40 g/L of lactose directly by microbial fermentation (Liu et al 2019).…”

Section: Production Of Galactitolmentioning

confidence: 99%

Sugar alcohols derived from lactose: lactitol, galactitol, and sorbitol

Zhang

Chen

et al. 2020

Appl Microbiol Biotechnol

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Lactose is a common natural disaccharide composed of galactose and glucose molecules. It is mainly found in the whey, the byproduct of cheese and casein industries. As the supply of lactose far exceeds demand, a lot of lactose was discarded as the waste every year, which not only leads to resource waste, but also causes environmental pollution. Therefore, the deep processing of lactose as the feedstock has become a hot research topic. The lactose-derived sugar alcohols, including lactitol, sorbitol, and galactitol, have shown great potential applications not only in food manufacture, but also in pharmaceutical, cosmetic, and material fields. In this paper, we focus on the property, physiological effect, production, and application of the lactose-derived sugar alcohols. Key points• The deep processing of lactose as the feedstock has become a hot research topic.• The lactose-derived sugar alcohols show great application values.• Recent advances in the lactose-derived sugar alcohols are reviewed.

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