Biotransformation of various natural sterols to androstenones by Mycobacterium sp. and some steroid-converting microbial strains

Sripalakit, Pattana; Wichai, Uthai; Saraphanchotiwitthaya, Aurasorn

doi:10.1016/j.molcatb.2006.04.007

Cited by 39 publications

(23 citation statements)

References 23 publications

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“…where m ad and m p are the weights of AD and PS, respectively; MW AD and MW p are the molecular weights of AD and PS, respectively (Sripalakit et al, 2006).…”

Section: Quantification Of Biotransformation Productmentioning

confidence: 99%

“…It is well-known that phytosterols (PSs) are suitable raw materials for microbial degradation to 17-ketosteroids because of low cost and easy availability (Fernandes and Cabral, 2007). In recent years, microbial selective cleavages of PS to AD and ADD have been reported by many researchers (Huang et al, 2006;Pérez et al, 2006;Sripalakit et al, 2006;Wang et al, 2006). All things considered, the major obstacle of microbial transformation of PS is the low aqueous solubility (1 μmol/L) of substrate (Malaviya and Gomes, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Zhang

Peng

et al. 2013

J. Zhejiang Univ. Sci. B

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Biotransformation of phytosterol (PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper. The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology. Androstenedione was the sole product in the fermentation broth catalyzed by the mutant M. neoaurum ZJUVN-08 strain. Results showed that molar ratio of hydroxypropyl-β-cyclodextrin (HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production. By analyzing the statistical model of three-dimensional surface plot, the optimal process conditions were observed at 0.1 g/L inducer, pH 7.0, molar ratio of HP-β-CD to PS 1.92:1, 8.98 g/L PS, and at 120 h of incubation time. Under these conditions, the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized (5.99 g/L), while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized (84.03%). The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results. It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process.

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“…where m ad and m p are the weights of AD and PS, respectively; MW AD and MW p are the molecular weights of AD and PS, respectively (Sripalakit et al, 2006).…”

Section: Quantification Of Biotransformation Productmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Zhang

Peng

et al. 2013

J. Zhejiang Univ. Sci. B

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“…The fundamental solution is to develop robust, efficient, highly productive microbial strains. Random mutagenesis is the conventional breeding approach and has been the main means of developing custom phytosterol-transforming strains (Egorova, et al, 2002;Donova, et al, 2005a;Sripalakit, et al, 2006). However, strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes.…”

Section: Development Of Ideal Organismsmentioning

confidence: 99%

From Soybean Phytosterols to Steroid Hormones

Wang¹,

Yao²,

Wei³

2011

Soybean and Health

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“…has been traditionally employed by many authors for the bioconversion of sterols for production of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD), the main precursors to produce steroidal drugs ( Fig. 1) (Marques et al 2009a;Pérez et al 2006;Rodina et al 2008;Sripalakit et al 2006;Voishvillo et al 2004). Nevertheless recently several reports have appeared on the use of free resting cells obtained by filtration or centrifugation of fermented broth (Carvalho et al 2009;Malaviya and Gomes 2008c;Marques et al 2007Marques et al , 2009bStaebler et al 2004;Cruz et al 2001;Wang et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of inoculation strategies, substrate to biomass ratio and nitrogen sources on the bioconversion of wood sterols by Mycobacterium sp.

Olivares

Acevedo

2011

World J Microbiol Biotechnol

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4-Androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) are the main precursors in the production of steroidal drugs from phytosterols. To carry out the bioconversion, different inoculation strategies have been proposed. We compared the use of whole fermented broth and of free resting cells of two mutant strains of Mycobacterium sp. (DSMZ2966 and DSMZ2967) in shake flasks. Also the effect of the nitrogen source (ammonium sulfate, ammonium chloride and ammonium nitrate) and the sterol to biomass ratio at high substrate concentrations (19.2 g/l and 48.1 g/l) was evaluated. We found that the bioconversion with free resting cells (cell pellets) is more efficient than that with whole fermented broth, increasing both AD and ADD production. The use of ammonium nitrate in the culture medium and low substrate to biomass ratios (close to 1.0) increased the production yield. We also found that the bioconversion can be run at high substrate concentration under non-sterile conditions.

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Biotransformation of various natural sterols to androstenones by Mycobacterium sp. and some steroid-converting microbial strains

Cited by 39 publications

References 23 publications

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

From Soybean Phytosterols to Steroid Hormones

Effect of inoculation strategies, substrate to biomass ratio and nitrogen sources on the bioconversion of wood sterols by Mycobacterium sp.

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