Biotransformation pathways of phytol in recent anoxic sediments

Grossi, Vincent; Hirschler, A; Raphel, Danielle; Rontani, Jean‐François; Leeuw, J.W. de; Bertrand, Jean Claude

doi:10.1016/s0146-6380(98)00118-1

Cited by 69 publications

(30 citation statements)

References 40 publications

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“…As such, phytol remains in the sediment as a recent record of senescent algal biomass or that which has been consumed and incorporated into biomass by heterotrophs. Phytol itself is also degraded in sediments, with a recent study showing that 85 to 90% of phytol will be degraded within 3 mo (Grossi et al 1998). In contrast, the turnover time for chlorophyll was estimated to be in the order of hours to days based on sediment chl a concentrations and estimated rates of primary production presented in Cook et al (2004a) which is consistent with published chl a degradation rates in sediments under oxic conditions (Sun et al 1993).…”

Section: Stable Isotopes As Indicators Of Sources Of Organic Carbon Wsupporting

confidence: 74%

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Cook¹,

Revill²,

Clementson³

et al. 2004

Mar. Ecol. Prog. Ser.

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The sources and cycling of organic matter on 2 mudflats in the Huon Estuary, SE Tasmania, were investigated over 4 seasons using pigment and lipid biomarkers as well as stable isotopes of carbon and nitrogen; 1 site was in the upper estuary,1 site was in a marine side-arm. The organic matter within the sediments at both sites was dominated by terrestrial sources, but most of this was refractory and only slowly remineralised. Significant, but seasonally variable, amounts of organic matter were derived from microphytobenthos (MPB), but this was rapidly remineralised and comprised only a minor fraction of the preserved sediment organic matter pool. Compound specific stable-isotope analysis of bacterial biomarkers suggested that the bacteria within the sediment were consuming microalgae-derived material. Separation of MPB from the sediment at the site in the upper estuary using the lens-tissue technique was found to significantly fractionate the algae community with cyanobacteria being enriched in the separated fraction. Nitrogen fixation probably contributed significantly to the growth requirements of cyanobacteria at the site in the upper estuary, as indicated by the low δ 15 N value of the cyanobacteria separated from the sediment.KEY WORDS: Microphytobenthos · Stable isotope · Compound-specific isotope-ratio mass spectrometry · Fatty acids · Sterols · Accessory pigment · Mudflat Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 280: [55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72] 2004 1999, Cahoon & Safi 2002), but this may shift seasonally to chlorophytes and cyanobacteria (Barranguet et al. 1997). In some areas, intertidal sediments may be dominated by cyanobacteria where high rates of nitrogen fixation may supply a significant amount of nitrogen to the benthic community (Stal et al. 1984, Bautista & Paerl 1985, Stal 1995.A number of approaches have been used to estimate the contributions of organic matter from various sources to sediments. Isotope ratios of 13 C/ 12 C have been used to estimate the relative contribution of terrestrial and marine sources to sedimentary organic matter (Fry & Sherr 1984). While this approach is relatively simple and gives an integrated estimate of sources for the total carbon in the sample, it will only provide useful information when there are 2 welldefined end-members. Furthermore, this technique gives little information about the type of marine or terrestrial organic matter in question.The use of lipid and pigment biomarkers such as fatty acids and sterols can allow the sources of various subfractions of organic matter to be identified much more specifically than any of the above approaches. However, unambiguously assigning sources to biomarkers is difficult, as they are rarely produced by a single class of organism. The advent of compound-specific stableisotope analysis has provided a powerful new tool for the differentiation between 2 sources of the same biomarker, provided the isotopic sign...

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Section: Stable Isotopes As Indicators Of Sources Of Organic Carbon Wsupporting

confidence: 74%

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Cook¹,

Revill²,

Clementson³

et al. 2004

Mar. Ecol. Prog. Ser.

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“…The hydration of double bonds seems common in the metabolisms of many organisms (32). This reaction has been hypothesized to occur during the anaerobic biotransformation of some isoprenoid alkenes (19,27) and during the mineralization of 1-hexadecene by a methanogenic enrichment culture that also continued to grow on primary long-chain alcohols (28). However, as far as we know, hydration of a double bond to yield a primary alcohol via an anti-Markovnikov-oriented reaction has never been proven to occur in an alkene-degrading anaerobic bacterium.…”

Section: Discussionmentioning

confidence: 99%

Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803 ^T

Grossi

Cravo‐Laureau

Méou³

et al. 2007

Appl Environ Microbiol

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The alkane-and alkene-degrading, marine sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T , known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6-to 10-week incubations allowed the identification of saturated 2-and 4-ethyl-, 2-and 4-methyl-, and monounsat-

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“…As a constituent of the photosynthetic pigment of plants, cyanobacteria, and algae (Volkman and Maxwell, 1986;Bauer et al, 1993), phytol and its derivatives can be used as indicators of photoautotrophic organisms and specific metabolic processes involved in the degradation of their biomass. Phytol is a common component of recent sediments (Rontani et al, 1996;Grossi et al, 1998), and it has been speculated that it is the major source of other isoprenoids of 20 or fewer carbon atoms in geological samples (Rontani and Volkman, 2003). The large quantity of phytol in the first layer ( Supplementary Fig.…”

Section: Fatty Acids Fatty Acid Biomarkers (Supplementarymentioning

confidence: 99%

Spatially Resolved Genomic, Stable Isotopic, and Lipid Analyses of a Modern Freshwater Microbialite from Cuatro Ciénegas, Mexico

et al. 2012

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Microbialites are biologically mediated carbonate deposits found in diverse environments worldwide. To explore the organisms and processes involved in microbialite formation, this study integrated genomic, lipid, and both organic and inorganic stable isotopic analyses to examine five discrete depth horizons spanning the surface 25 mm of a modern freshwater microbialite from Cuatro Ciénegas, Mexico. Distinct bacterial communities and geochemical signatures were observed in each microbialite layer. Photoautotrophic organisms accounted for approximately 65% of the sequences in the surface community and produced biomass with distinctive lipid biomarker and isotopic (d 13 C) signatures. This photoautotrophic biomass was efficiently degraded in the deeper layers by heterotrophic organisms, primarily sulfate-reducing proteobacteria. Two spatially distinct zones of carbonate precipitation were observed within the microbialite, with the first zone corresponding to the phototroph-dominated portion of the microbialite and the second zone associated with the presence of sulfatereducing heterotrophs. The coupling of photoautotrophic production, heterotrophic decomposition, and remineralization of organic matter led to the incorporation of a characteristic biogenic signature into the inorganic CaCO 3 matrix. Overall, spatially resolved multidisciplinary analyses of the microbialite enabled correlations to be made between the distribution of specific organisms, precipitation of carbonate, and preservation of unique lipid and isotopic geochemical signatures. These findings are critical for understanding the formation of modern microbialites and have implications for the interpretation of ancient microbialite records.

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Biotransformation pathways of phytol in recent anoxic sediments

Cited by 69 publications

References 40 publications

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803 ^T

Spatially Resolved Genomic, Stable Isotopic, and Lipid Analyses of a Modern Freshwater Microbialite from Cuatro Ciénegas, Mexico

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Biotransformation pathways of phytol in recent anoxic sediments

Cited by 69 publications

References 40 publications

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803 T

Spatially Resolved Genomic, Stable Isotopic, and Lipid Analyses of a Modern Freshwater Microbialite from Cuatro Ciénegas, Mexico

Contact Info

Product

Resources

About

Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803 ^T