Biotype Characterization, Developmental Profiling, Insecticide Response and Binding Property of Bemisia tabaci Chemosensory Proteins: Role of CSP in Insect Defense

Liu, Guoxia; Ma, Hongmei; Xie, Hongyan; Xuan, Ning; Guo, Xia; Zhong-xue, Fan; Rajashekar, Balaji; Arnaúd, Philippe; Offmann, Bernard; Picimbon, Jean-François

doi:10.1371/journal.pone.0154706

Cited by 62 publications

(156 citation statements)

References 91 publications

(109 reference statements)

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“…We know little about this gene other than it has been observed to be expressed in queen haemolymph (Sadd et al, ), and carries an odorant binding domain, suggesting that it may play a role in the transportation of semiochemicals such as odours and pheromones in the haemolymph. Its role may be conserved as a homologous gene in the whitefly Bemisia tabaci is also upregulated after exposure to the neonicotinoid thiamethoxam (Liu et al, ). Several general functions (Gene Ontology terms) were shared by workers and queens, including oxidation–reduction process, glucose metabolic process and single‐organism catabolic process (Supporting information Figure ).…”

Section: Discussionmentioning

confidence: 99%

Caste‐ and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

et al. 2019

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Social bees are important insect pollinators of wildflowers and agricultural crops, making their reported declines a global concern. A major factor implicated in these declines is the widespread use of neonicotinoid pesticides. Indeed, recent research has demonstrated that exposure to low doses of these neurotoxic pesticides impairs bee behaviours important for colony function and survival. However, our understanding of the molecular‐genetic pathways that lead to such effects is limited, as is our knowledge of how effects may differ between colony members. To understand what genes and pathways are affected by exposure of bumblebee workers and queens to neonicotinoid pesticides, we implemented a transcriptome‐wide gene expression study. We chronically exposed Bombus terrestris colonies to either clothianidin or imidacloprid at field‐realistic concentrations while controlling for factors including colony social environment and worker age. We reveal that genes involved in important biological processes including mitochondrial function are differentially expressed in response to neonicotinoid exposure. Additionally, clothianidin exposure had stronger effects on gene expression amplitude and alternative splicing than imidacloprid. Finally, exposure affected workers more strongly than queens. Our work demonstrates how RNA‐Seq transcriptome profiling can provide detailed novel insight on the mechanisms mediating pesticide toxicity to a key insect pollinator.

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Section: Discussionmentioning

confidence: 99%

Caste‐ and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

et al. 2019

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“…Before the current study, the cDNA sequences of OBPs and CSPs had been only partly identified in B. tabaci (only eight OBP and 13 CSP sequences had been identified in MED, and only three CSP sequences had been identified in MEAM1), and especially in many cases, the sequences were incomplete (Li et al ., ; Liu et al ., , ; Wang et al ., , , ). In the current study, we obtained complete sequences for the eight OBPs and 19 CSPs in MEAM1 (Table ; Table ; Table S3) based on genome and antennae transcriptome data sets; these covered (and in some cases completed) all previously published sequences, and added six CSP sequences.…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide analysis of odorant‐binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci

Zeng

Yang

et al. 2018

Insect Science

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice, copulation, reproduction and other behaviors. Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information. In this study, we present a genome-wide and transcriptome-wide investigation of the OBPs and CSPs in B. tabaci MEAM1 (Middle East-Asia Minor1 species). Eight OBP and 19 CSP genes were identified that covered all previous sequences. Phylogenetic analyses showed that the CSP genes had a lineage-specific expansion (BtabBCSP1, BtabBCSP3, BtabBCSP13, BtabBCSP17, BtabBCSP18 and BtabBCSP19). Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real-time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages of B. tabaci MEAM1. Five OBP genes and 11 CSP genes significantly differed between males and females; four of the 19 CSP genes were highly expressed in adults, while two were highly expressed in nymphs. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MEAM1 adults were analyzed by qPCR. Four OBP genes found in B. tabaci MEAM1 were highly expressed in the head. Conversely, only two CSPs were enriched in the head, while the other six CSPs were specifically expressed in other tissues. Our results provide a foundation for future research on OBPs and CSPs in B. tabaci.

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“…Liu et al., 2014 b ; Zhu et al., 2016 a ); (17) H elicoverpa armigera and other species (Zhu et al., 2016 a ); (18) B ombyx mori (Xuan et al., ); (19) B emisia tabaci (G.X. Liu et al., 2014 a , 2016); P lutella xylostella (Bautista et al., ).…”

Section: Multiple Functions Of Obps and Cspsmentioning

confidence: 99%

“…Liu et al, 2014b;Zhu et al, 2016a); (17) Helicoverpa armigera and other species (Zhu et al, 2016a); (18) Bombyx mori (Xuan et al, 2015); (19) Bemisia tabaci (G.X. Liu et al, 2014aLiu et al, , 2016; Plutella xylostella (Bautista et al, 2015). while the only CSP found in reproductive organs of male locusts is also expressed in chemosensory structures, such as antennae, mouthparts and tarsi .…”

Section: (2) Pheromone Glands: Releasing Semiochemicalsmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.

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Biotype Characterization, Developmental Profiling, Insecticide Response and Binding Property of Bemisia tabaci Chemosensory Proteins: Role of CSP in Insect Defense

Cited by 62 publications

References 91 publications

Caste‐ and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

Caste‐ and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

Genome‐wide analysis of odorant‐binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

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