Biotypes and O serogroups of Escherichia coli involved in intestinal infections of weaned rabbits: clues to diagnosis of pathogenic strains

Camguilhem, R.; Milon, A.

doi:10.1128/jcm.27.4.743-747.1989

Cited by 61 publications

(32 citation statements)

References 12 publications

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“…AF/R2 also appears to be encoded by some other REPEC serotypes (Penteado et al, 2002). Experimentally, REPEC O103 strains caused watery or haemorrhagic diarrhoea in weaned rabbits (Camguilhem and Milon, 1989) and elaborated fimbriae transiently in vivo (Heczko et al, 2000).…”

Section: Rabbitsmentioning

confidence: 99%

“…coli O103, and particularly the O103:K-:H2 serotype, is the predominant REPEC reported from France and Spain (Camguilhem and Milon, 1989;Blanco et al, 1996Blanco et al, , 1997. Virulence is closely associated with the rhamnose non-fermenting biotype (Camguilhem and Milon, 1989) that encodes chromosomal genes for a fimbrial-type adhesin termed AF/R2, which is related to both the ETEC K88 adhesin and the ral-encoded adhesin of REPEC O15 (Pillien et al, 1996;Fiederling et al, 1997). AF/R2 also appears to be encoded by some other REPEC serotypes (Penteado et al, 2002).…”

Section: Rabbitsmentioning

confidence: 99%

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Attaching-effacing Bacteria in Animals

Wales

Woodward²,

Pearson

2005

Journal of Comparative Pathology

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Enteric bacteria with a demonstrable or potential ability to form attaching-effacing lesions, so-called attaching-effacing (AE) bacteria, have been found in the intestinal tracts of a wide variety of warm-blooded animal species, including man. In some host species, for example cattle, pigs, rabbits and human beings, attaching-effacing Escherichia coli (AEEC) have an established role as enteropathogens. In other host species, AE bacteria are of less certain significance. With continuing advances in the detection and typing of AE strains, the importance of these bacteria for many hosts is likely to become clearer. The pathogenic effects of AE bacteria result from adhesion to the intestinal mucosa by a variety of mechanisms, culminating in the formation of the characteristic intimate adhesion of the AE lesion. The ability to induce AE lesions is mediated by the co-ordinated expression of some 40 bacterial genes organized within a so-called pathogenicity island, known as the "Locus for Enterocyte Effacement". It is also believed that the production of bacterial toxins, principally Vero toxins, is a significant virulence factor for some AEEC strains. Recent areas of research into AE bacteria include: the use of Citrobacter rodentium to model human AEEC disease; quorum-sensing mechanisms used by AEEC to modulate virulence gene expression; and the potential role of adhesion in the persistent colonization of the intestine by AE bacteria. This review of AE bacteria covers their molecular biology, their occurrence in various animal species, and the diagnosis, pathology and clinical aspects of animal diseases with which they are associated. Reference is made to human pathogens where appropriate. The focus is mainly on natural colonization and disease, but complementary experimental data are also included.

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Section: Rabbitsmentioning

confidence: 99%

Section: Rabbitsmentioning

confidence: 99%

Attaching-effacing Bacteria in Animals

Wales

Woodward²,

Pearson

2005

Journal of Comparative Pathology

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“…EPEC infections are also responsible for diarrhea outbreaks in numerous animal species. Just weaned rabbits are particularly sensitive to EPEC infections, leading to considerable economical losses in industrial fattening farms of Western Europe (2, 3). Because EPEC strains isolated from humans do not cause diarrhea in animals, it is difficult to find a good animal model to study human infections.…”

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confidence: 99%

New flow cytometric method to quantify the inhibition of enteropathogenic Escherichia coli adhesion by anti‐adhesin antibodies

2003

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Background: Pathogenesis of enteropathogenic Escherichia coli (EPEC) infections can be divided in three stages. The first one is the intestinal colonization mediated by bacterial adhesins. The second and third stages are characterized by an intimate attachment of bacteria to the enterocytes. Little information is available on the specific immune response against EPEC. Here, we describe and validate a new approach to quantify the function of anti-EPEC adhesin antibodies (Abs). Methods: We developed a new method to quantify the function of anti-adhesin Abs by flow cytometry. We used pEGFP-E22 (a rabbit EPEC E22 strain expressing the GFP protein) and HeLa cells. The adhesion of E22 bacteria to HeLa cells is mediated by AF/R2, the specific E22 adhesin. We performed short-time interaction (30 min) between pEGFP-E22 and HeLa cells. After extensive washes, 10,000 HeLa cells were acquired by flow cytometry and bacterial adhesion was quantified. Different sera were used to inhibit bacterial adhesion and recombinant MPB-Afr2G (Afr2G is the main AF/R2 subunit) was also tested in this system.

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“…In France, EPEC strains belonging to serogroup 015 rarely have been found up to now (4), but E. coli strains belonging to serogroup 0103 have been isolated in 30 to 40% of diarrheic rabbits (5, lla). It has been demonstrated previously that some strains of E. coli 0103 are highly virulent for rabbits (2,17,26).…”

mentioning

confidence: 99%

Scanning and transmission electron microscopic study of adherence of Escherichia coli O103 enteropathogenic and/or enterohemorrhagic strain GV in enteric infection in rabbits

Licois¹,

Reynaud²,

Fédérighi³

et al. 1991

Infect Immun

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The GV strain (serotype 0103:H2:K-), originally isolated from a diarrheic rabbit, is an enteropathogenic Escherichia coli strain that produces diarrhea without synthesizing the classical enterotoxins and that is not invasive. This strain is characterized by a 117-kb plasmid (pREC-l). Histological study of the gut by scanning electron microscopy and transmission electron microscopy was performed on the GV strain, on a derivative strain cured of pREC-l, and on transconjugants obtained by transfer of pREC-l to nonpathogenic strains E. coli K-12 and 6100, not belonging to the 0103 serogroup. The GV strain adhered to the epithelial cells of the ileum and large intestine, whereas the cured GV strain did not. Transfer of plasmid pREC-1 to E. coli K-12 or 6100 allowed the bacteria to attach to the intestinal mucosa in the same manner as that of the wild-type GV strain. Thus, pREC-l seems to play an important role in attachment to and colonization of the intestinal tract of rabbits by E. coli serogroup 0103. Scanning electron microscopy showed numerous bacteria attached together and closely associated with intestinal villi. Transmission electron microscopy revealed effacing lesions characteristic of enteropathogenic E. coli strains: effacing of microvilli and cuplike projections (pedestal formations) associated with an acute inflammatory and hemorrhagic response. In contrast with the results reported for rabbit pathogenic 015 strains, it appeared that the Peyer's patches were not involved in the early stages of infection with the 0103 GV strain. This strain may represent a model for the study of the virulence and pathogenic effects of enteropathogenic and enterohemorrhagic E. coli strains.

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Biotypes and O serogroups of Escherichia coli involved in intestinal infections of weaned rabbits: clues to diagnosis of pathogenic strains

Cited by 61 publications

References 12 publications

Attaching-effacing Bacteria in Animals

Attaching-effacing Bacteria in Animals

New flow cytometric method to quantify the inhibition of enteropathogenic Escherichia coli adhesion by anti‐adhesin antibodies

Scanning and transmission electron microscopic study of adherence of Escherichia coli O103 enteropathogenic and/or enterohemorrhagic strain GV in enteric infection in rabbits

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