Biotyping of Escherichia coli by a simple multiple-inoculation agar plate technique

Buckwold, Frederick J.; Ronald, Allan R.; Harding, G. K. M.; Marrie, Tom; L, Fox; Cates, C. Y.

doi:10.1128/jcm.10.3.275-278.1979

Cited by 27 publications

(9 citation statements)

References 5 publications

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“…Escherichia coli with pathogenic virulence markers and representative isolates from different sample sources were biotyped using a modified method of Buckwold, Ronald, Harding, Marrie, Fox and Cates (1979). Briefly, the biotyping system consisted of 12 carbohydrate fermentation tests, divided into four groups of three tests: fermentation of maltose, sucrose and dulcitol; fermentation of trehalose, raffinose and salicin; fermentation of lactose, arabinose and cellobiose; and fermentation of xylose, sorbitol and inositol.…”

Section: Methodsmentioning

confidence: 99%

Virulence genes, serobiotypes and antibiotic resistance profile ofEscherichia colistrains isolated from aquaculture and other sources

Surendraraj

Thampuran

Joseph

2009

Aquaculture Research

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In order to determine the prevalence of pathogenic Escherichia coli, a total number of 155 E. coli isolates from aquaculture, clinical and veterinary sources were screened for seven pathogenic virulence markers and a house-keeping gene by a polymerase chain reaction. The targeted virulence genes included eaeA of enteropathogenic E. coli, elt and est of enterotoxigenic E. coli (ETEC), ipaH of enteroinvasive E. coli, pCVD432 of enteroaggregative E. coli, stx, hlyA and eaeA of shigatoxigenic E. coli (STEC) and Enterohaemorrhagic E. coli. All the isolates were positive for phoA, the house-keeping gene for E. coli. Among the 155 isolates, seven numbers (4.5%) harboured the virulence markers belonging to the pathogenic group ETEC and STEC. The virulent genes detected in these groups were elt, est, hlyA and stx. The sources of these virulence genes were ¢sh (hlyA), shrimp (elt), feeder canal water (hlyA and elt) of aquaculture origin and from diarrhoea a¡ected cow (hlyA, est and stx). The isolates with pathogenic traits belonged to the serogroups O6 or O29 and the remaining could not be typed. They showed resistance to two to four antibiotics out of the 12 antibiotics tested. Biotyping revealed that three isolates belonged to a single biotype (7333) and the remaining isolates were of diverse types. In conclusion, a molecular tool such as PCR proves as more e¡ective tool for detection of this pathogen than the conventional methods. Detection of these emerging pathogens in aquaculture samples warrants for strict adherence to hygienic handling at retail outlets and proper cooking by the consumer before consumption.

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Section: Methodsmentioning

confidence: 99%

Virulence genes, serobiotypes and antibiotic resistance profile ofEscherichia colistrains isolated from aquaculture and other sources

Surendraraj

Thampuran

Joseph

2009

Aquaculture Research

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“…Bacteriuria was continuous in one patient, and superinfection developed in another. Cure (defined as the finding of less than 10 4 cfu/ml of urine in a six‐weeks post‐treatment, properly collected specimen) was observed in only one instance. Among patients with documented recurrences six weeks after the discontinuation of drug therapy, three showed bacterial relapse (a measure of drug failure), and six showed re‐infections.…”

Section: Resultsmentioning

confidence: 99%

“…Quantitative analysis for bacteriuria was carried out by the calibrated loop technique. Biotyping of Escherichia coli was performed to differentiate bacterial persistence (relapse) from reinfection in patients with recurrent E. coli urinary tract infections 4 . Results of the antibody‐coated bacterial (ACB) immunofluorescence test to localize the tissue site of a urinary‐tract infection were considered positive when more than two uniformly fluorescent bacteria were seen during microscopic examination of the urinary sediment for a minimum of 5 minutes 5 …”

Section: Methodsmentioning

confidence: 99%

Catheter‐related Urosepsis in the Elderly: A Prospective Study of Community‐derived Infections

Gleckman

Blagg

Hibert

et al. 1982

J American Geriatrics Society

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For the period 1979-1980, a prospective study was made of 13 elderly patients with catheter-related urosepsis, who required hospitalization. Several features appeared to distinguish these patients: (1) a traumatic catheter-related event invariably preceded the acute symptomatic process, (2) polymicrobic bacteriuria predominated, and (3) the urinary pathogens were often resistant to the antimicrobial agents commonly prescribed for communityassociated symptomatic urinary-tract infections. A diagnostic and therapeutic approach for these patients is outlined.The infected urinary tract has been identified as the most common source for bacteremia in elderly patients admitted to a community hospital.' For the elderly patient, acute symptomatic urosepsis often necessitates hospitalization and occasionally results in death. In the present prospective study we assessed the clinical, bacteriologic, immunologic, and the rape u t i c feature s of s y m p t o m at i c , catheterassociated urosepsis as experienced by 13 consecutive elderly patients who required hospital admission. Our intent was to focus on the features of the disease that appear to be unique to the elderly catheterized patient, and to outline a diagnostic and therapeutic approach for these patients. MATERIALS AND METHODSThis prospective study commenced in December 1979 and was concluded in November 1980. During this period, 4955 patients aged 65 or older were discharged from the medical and surgical services of the hospital.The study group consisted of 13 symptomatic, elderly white febrile patients with indwelling bladder-catheters who were admitted to the hospital because of community-acquired urosepsis. Fever, constitutional symptoms, and "significant" bacteriuria (more than 105 colony-forming units per ml of a recognized urinary pathogen) were required criteria for participation in the study. Patients were excluded if another infection, a neoplasm, or an inflammatory disorder existed that could provide an alternative explanation for the admission fever and symptoms.Vincent Hospital, a 650-bed community hospital and major teaching affiliate of the University of Massachusetts Medical School. The patients' private physicians arid the members of the medical house staff made all the diagnostic and therapeutic decisions.The patients were classified according to the severity of their associated medical illnesses? Identification of septic shock required the presence of fever, altered mental status, and a systolic blood pressure less than 90 mm Hg.3 Patients known to be hypertensive before hospitalization were considered to have sustained shock if their admission systolic blood pressures fell by more than 50 mm Hg.The study received the approval of the institutional review committee for human research of Saint Vincent Hospital. Statistical differences between groups were analyzed with Student's twotailed t-test.Quantitative analysis for bacteriuria was carried out by the calibrated loop technique. Biotyping of Escherichia coli was performed to differentiate bacteria...

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“…Thirty-six strains were tested on a single 9 cm petri dish for each carbohydrate except aesculin where it was found to be more appropriate to test 20 strains on a plate. It was not found necessary to cut the agar between the inocula as suggested by Buckwold et al (1979).…”

Section: Inoculation and Incubation Of Mediamentioning

confidence: 99%

“…A number of schemes have been reported for differentiating (biotyping) strains of Escherichia coli isolated from human patients (particularly those suffering from urinary infections) based on the investigation of their antigenic properties, metabolic activity, morphology and resistance to either antibacterial drugs or chemicals (Bettleheim & Taylor, 1969;Elek & Hignay, 1970;Davies, 1977;Buckwold et al 1979;Crichton & Old, 1979;Old et al 1980. ) One typing method alone is often not satisfactory for confirming the identity of strains, but the use of methods in combination makes identification more accurate (Old et al 1980).…”

Section: Introductionmentioning

confidence: 99%

The biotyping ofEscherichia coliisolated from healthy farm animals

Hinton

Allen

Linton

1982

J. Hyg.

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A total of 2973 Escherichia coli, isolated from six different groups of animals, were examined for their ability to ferment adonitol, dulcitol, raffinose, rhamnose and sorbose in solid media. Twenty-nine fermentation patterns were recorded although 2443 (82%) of the E. coli belonged to seven of the 32 possible biotypes. Ninety-six O-serotypes were identified within the 2973 E. coli. The number of O-serotypes represented in the 15 most common biotypes ranged from three to 15. Serotypes O8 and O9 were found most commonly in the different groups of animals and several biotypes amongst these two O-serotypes were identified in two or more groups of the animals. The ability of the E. coli to metabolize aesculin, ornithine, salicin and sucrose was also assessed. These test proved less reproducible and were not included in the primary biotyping scheme although their use allowed the enumeration of additional biotypes. The application of biotyping to the study of the ecology of drug-resistant strains of E. coli in five situations is briefly presented.

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Biotyping of Escherichia coli by a simple multiple-inoculation agar plate technique

Cited by 27 publications

References 5 publications

Virulence genes, serobiotypes and antibiotic resistance profile ofEscherichia colistrains isolated from aquaculture and other sources

Virulence genes, serobiotypes and antibiotic resistance profile ofEscherichia colistrains isolated from aquaculture and other sources

Catheter‐related Urosepsis in the Elderly: A Prospective Study of Community‐derived Infections

The biotyping ofEscherichia coliisolated from healthy farm animals

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