Bioutilization of Chicken Feather Wastes by Newly Isolated Keratinolytic Bacteria Into Protein Hydrolysates With Improved Functionalities

Abstract

In this study, a novel feather-degrading bacteria B. amyloliquefaciens KB1 was isolated from chicken farm bed (CFB), identified by morphological, physico-biochemical tests followed by 16s rDNA analysis. Among observed isolates, bacterial isolate (KB1) showed the highest degree of feather degradation (74.78 ± 2.94 %) and total soluble protein (205 ± 0.03 mg/ g). Using the same species of bacteria, the optimum fermentation condition was found at 40 oC, pH 9, and 1 % (w/v) feather concentration that produced 260 mg/ g of soluble protein and 86.16 % feather degradation using response surface methodology in a Box-Behnken design space. The obtained hydrolysates exhibited bioactive properties. The amino acid profile showed the increase in concentration of essential amino acid compared with feather meal broth. The selection of safe screening source of this new bacteria in CFB produced hydrolysates with enhanced bioactivity applicable for food, feed, and cosmetic applications along with environmental remediation.

Protease Catalyzed Production of Spent Hen Meat Hydrolysate Powder for Health Food Applications

“…e generated hydrolysates have been shown to possess enhanced nutritional and therapeutic activities including but not limited to antioxidant activity [3,4], antihypertensive activity [5], hypoglycaemic effect [6], antimicrobial properties [7], and antitumor activity [8]. Functional characteristics of these hydrolysates are due to bioactive peptides formed during hydrolysis of native proteins.…”