Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration

Liu, Pei; Li, Mengna; Yu, Hongping; Fang, Haoyu; Yin, Junhui; Zhu, Daoyu; Yang, Qianhao; Ke, Qinfei; Huang, Yigang; Guo, Ya‐Jun; Gao, You-Shui; Zhang, Changqing

doi:10.1016/j.cej.2021.129531

Cited by 21 publications

(24 citation statements)

References 74 publications

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“… 56 , 72 Higher temperatures trigger the transfer of protons from chitosan’s −NH 3 + groups to the GP backbone, reducing the charge density of chitosan and favoring hydrophobic interchain interactions and hydrogen bonding between chitosan chains, resulting in the formation of a gel. 57 , 58 , 72 , 73 …”

Section: Resultsmentioning

confidence: 99%

“…56,72 Higher temperatures trigger the transfer of protons from chitosan's −NH 3 + groups to the GP backbone, reducing the charge density of chitosan and favoring hydrophobic interchain interactions and hydrogen bonding between chitosan chains, resulting in the formation of a gel. 57,58,72,73 We investigated the gelling behavior of chitosan/GP mixtures featuring three different molecular weights of chitosan (LMW = 250 kDa, MMW = 1250 kDa, and HMW = 1500 kDa) and various concentrations of CPMV (0−4.5 mg mL −1 ) at 37 °C. The gelation time was assessed by the flow and turbidity of each mixture following tube inversion (Figure 2A).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Chitosan is soluble in acids due to the electrostatic repulsion between its positively charged amine-protonated chains. The addition of GP neutralizes the solution (pH = 6.5–7.3) without inducing immediate precipitation or aggregation because GP deprotonates some of chitosan’s positively charged amine groups (−NH 3 + ), allowing electrostatic attraction between the GP backbone and chitosan’s remaining −NH 3 + groups, in turn exposing the glycerol moiety of GP to neighboring chitosan chains and enhancing their solubility when the temperature is below ∼23 °C. , Higher temperatures trigger the transfer of protons from chitosan’s −NH 3 + groups to the GP backbone, reducing the charge density of chitosan and favoring hydrophobic interchain interactions and hydrogen bonding between chitosan chains, resulting in the formation of a gel. ,,, …”

Section: Resultsmentioning

confidence: 99%

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Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

et al. 2022

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Cowpea mosaic virus (CPMV) is a potent immunogenic adjuvant and epitope display platform for the development of vaccines against cancers and infectious diseases, including coronavirus disease 2019. However, the proteinaceous CPMV nanoparticles are rapidly degraded in vivo. Multiple doses are therefore required to ensure long-lasting immunity, which is not ideal for global mass vaccination campaigns. Therefore, we formulated CPMV nanoparticles in injectable hydrogels to achieve slow particle release and prolonged immunostimulation. Liquid formulations were prepared from chitosan and glycerophosphate (GP) before homogenization with CPMV particles at room temperature. The formulations containing high-molecular-weight chitosan and 0–4.5 mg mL –1 CPMV gelled rapidly at 37 °C (5–8 min) and slowly released cyanine 5-CPMV particles in vitro and in vivo. Importantly, when a hydrogel containing CPMV displaying severe acute respiratory syndrome coronavirus 2 spike protein epitope 826 (amino acid 809–826) was administered to mice as a single subcutaneous injection, it elicited an antibody response that was sustained over 20 weeks, with an associated shift from Th1 to Th2 bias. Antibody titers were improved at later time points (weeks 16 and 20) comparing the hydrogel versus soluble vaccine candidates; furthermore, the soluble vaccine candidates retained Th1 bias. We conclude that CPMV nanoparticles can be formulated effectively in chitosan/GP hydrogels and are released as intact particles for several months with conserved immunotherapeutic efficacy. The injectable hydrogel containing epitope-labeled CPMV offers a promising single-dose vaccine platform for the prevention of future pandemics as well as a strategy to develop long-lasting plant virus-based nanomedicines.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

et al. 2022

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“…Other applications of LDHs in tissue engineering are summarized in Table 3. 463–476 However, further research effort should be devoted to evaluating the clinical application of LDHs in regenerative medicine comprehensively.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Layered double hydroxide-based nanomaterials for biomedical applications

et al. 2022

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“…Results show that in vivo assays PMMA&LDH has been demonstrated superior osteogenic ability, which boosts bone growth by 2.17-and 18.34-fold increments compared to other groups without LDH at 2 months, postoperatively 25 . In a similar study, Liu et al 26 fabricated an integrated strategy involving mesenchymal stem cells (MSCs) recruitment, and multifunctional therapeutic biomaterials of bone regeneration and vascularization containing a bilayer peptide-loaded scaffold consisting of CK2.1 coated β-glycerophosphate/ chitosan (CK2.1@GC) for cartilage regeneration and LL37 modified layered double hydroxide/chitosan (LL37@LC) for subchondral bone regeneration 26 . Drug-loaded-LDHs incorporated in biopolymers as composite for bone tissue application are also recently investigated.…”

Section: Introductionmentioning

confidence: 99%

Ibuprofen Release from Hydrotalcite-like Materials Filled into Chitosan/Alginate Composites as Promising Reabsorbable Membranes

et al. 2021

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Hydrotalcite-type materials, or layered double hydroxide (LDH), are very promising in applying slow-release drug systems through ion exchange and delamination characteristics at acidic pH. In this paper, Zn/Al LDHs in 2:1 and 3:1 molar ratios intercalated with the drug ibuprofen (IBU) were added to chitosan/alginate membranes. In the release tests, an increase in the solubility of the drug in the membranes could be observed, and the percentage of drug release is lower than the percentage of drug release in the free samples. The following release percentages were obtained: 15.48% and 17.5% of the drug in water, and 23.22% and 26.25% of the drug in PBS buffer solution, respectively, for membrane samples containing Zn 2 Al-IBU and Zn 3 Al-IBU LDHs. Thus, the synthesized hybrid membranes (chitosan/alginate-LDH-IBU) are promising in the application as resorbable membranes for bone grafting with drug release.

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Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration

Cited by 21 publications

References 74 publications

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

Layered double hydroxide-based nanomaterials for biomedical applications

Ibuprofen Release from Hydrotalcite-like Materials Filled into Chitosan/Alginate Composites as Promising Reabsorbable Membranes

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