Biphasic dose–response of antioxidants in hypericin-induced photohemolysis

Martirosyan, Alina; Vardapetyan, Hrachik; Tiratsuyan, S. G.; Hovhannisyan, A.

doi:10.1016/j.pdpdt.2011.03.339

Cited by 13 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It may be suggested that at higher fluence rates, increased radical concentration may favour radical quenching through intermolecular interactions resulting in formation of non-active adducts before the PD effect may be exerted, or implies fast degradation of cellularly incorporated curcumin. 47,48 Tanaka, Hamblin et al observed a similar behavior when they examined the effect of Photofrin-mediated photodynamic therapy on bacterial arthritis in mouse model. 49,50 After application of PDT for the treatment of an MRSA arthritis infection in the mouse knee (1 μg Photofrin, 635 nm diode laser illumination), a biphasic light dose response occurred.…”

Section: Papermentioning

confidence: 71%

Curcumin derivatives as photosensitizers in photodynamic therapy: photophysical properties and in vitro studies with prostate cancer cells

Kazantzis

Koutsonikoli

Mavroidi

et al. 2020

Photochem Photobiol Sci

106

View full text Add to dashboard Cite

show abstract

Section: Papermentioning

confidence: 71%

Curcumin derivatives as photosensitizers in photodynamic therapy: photophysical properties and in vitro studies with prostate cancer cells

Kazantzis

Koutsonikoli

Mavroidi

et al. 2020

Photochem Photobiol Sci

106

View full text Add to dashboard Cite

show abstract

“…In addition to the modulatory and antioxidant effects, it has been reported that some bioflavonoids possess anti‐androgenic properties in vivo depending on their bioavailability and kinetics related to metabolism and bio‐transformation (Das et al ., ). Hence, they may display strong antioxidants or pro‐oxidant properties depending on the physiological milieu and potential interactions thereof (Laughton et al ., ; Aruoma et al ., ; Lee & Lee, ; Martirosyan et al ., ). This may have been responsible for the diverse effects on the longer tail length observed in HN80 when compared to HN40.…”

Section: Discussionmentioning

confidence: 97%

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

et al. 2017

View full text Add to dashboard Cite

SUMMARYHighly active antiretroviral therapy has evolved over the years, leading to a boost in the quality of life in people living with HIV and AIDS. However, growing evidence has shown that highly active antiretroviral therapy has deleterious effects on the testes and the overall reproductive capacity. Therefore, this study is to determine the adjuvant potential of Naringenin on highly active antiretroviral therapy-induced perturbations in fertility of male Sprague-Dawley rats. Thirty adult male Sprague-Dawley rats were divided into six groups viz -Control; H: 30 mg/kg of highly active antiretroviral therapy (EFV, 600 mg + FTC, 200 mg + TDF, 300 mg); N40: Naringenin, 40 mg/kg; N80: Naringenin, 80 mg/kg; HN40: highly active antiretroviral therapy + Naringenin, 40 mg/kg; HN80: highly active antiretroviral therapy + Naringenin, 80 mg/kg. The rats were euthanized after 4 weeks. Results showed that there was a significant decrease in sperm count (p < 0.001), spermatozoa with normal morphology (p < 0.001) and progressive sperm motility (p < 0.05) of H compared to the control and the HN groups. Likewise, fragmentations increased (p < 0.05) in tail lengths of sperm DNA in H compared to control. HN40 and HN80 decreased tail lengths compared to H (p < 0.001). There was also a decrease in %tail DNA and tail moment in HN40 (p < 0.001) compared to H. Luteinizing hormone significantly increased (p < 0.05) in HN40, HN80, and N40 (p < 0.001) but decreased in H (p < 0.05) compared to control. The diameter of the seminiferous tubules also decreased (p < 0.05) in H compared to control, N80, and HN40. Likewise, the area of the seminiferous tubules in group H decreased (p < 0.05) compared to N80 and HN80. The seminiferous tubules epithelium increased (p < 0.05) in N40 and HN40 compared to H. This study establishes that highly active antiretroviral therapy has deleterious effects on the testicular microanatomy, sperm parameters, and sperm DNA of Sprague-Dawley rats, which may impair fertility but Naringenin is a potential complimentary adjuvant.

show abstract

“…Photodynamic therapy (PDT) is a cytotoxic treatment induced by light interactions, cell or tissue molecular oxygen and photosensitizing molecules, termed the photosensitizer (PS) ( 1 ). Once irradiated by visible light, the PS can react through two photo-oxidative pathways, classified as type I and type II ( 2 ). The type I pathway involves the generation of free radicals through PS or a substrate.…”

Section: Introductionmentioning

confidence: 99%

Hypericin-mediated photodynamic therapy induces apoptosis in K562 human leukemia cells through JNK pathway modulation

Wang

Zhuang

et al. 2015

Molecular Medicine Reports

View full text Add to dashboard Cite

Hypericin (Hyp) is traditionally used as an antidepressant and antiviral agent. It selectively accumulates in spheroids and is also used as a photosensitizer in the photodynamic therapy of cancer. The present study aimed to investigate the cytotoxic effect of Hyp-mediated photodynamic therapy (Hyp-PDT) on cell growth and apoptosis of K562 leukemia cells, and to examine the underlying mechanisms. Hyp-PDT was performed with different light intensities (0.1, 0.3 and 0.5 mW/cm2), different concentrations of Hyp (0, 0.2, 0.4 and 0.8 µg/ml) and different durations of irradiation (0, 2, 4 and 8 min) in order to select the optimal conditions for subsequent experiments. A concentration of 0.4 µg/ml Hyp with a 5 h drug-light interval and 4 min irradiation at 0.3 mW/cm2 light intensity was selected as the optimal conditions. The effects of Hyp-PDT on apoptosis were determined by detecting morphological changes under microscopy and by performing western blot analysis. The results revealed that Hyp-PDT suppressed cell viability in a light intensity-, dose- and irradiation duration-dependent manner. The expression levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated-C-Jun N terminal kinase (JNK) l were significantly upregulated following Hyp-PDT. These results indicated that Hyp-PDT decreased cell viability and induced mitochondria-caspase-dependent apoptosis in the K562 cells through regulation of the JNK pathway. These findings suggest that Hyp-PDT may be developed as an effective treatment for leukemia.

show abstract

Biphasic dose–response of antioxidants in hypericin-induced photohemolysis

Cited by 13 publications

References 24 publications

Curcumin derivatives as photosensitizers in photodynamic therapy: photophysical properties and in vitro studies with prostate cancer cells

Curcumin derivatives as photosensitizers in photodynamic therapy: photophysical properties and in vitro studies with prostate cancer cells

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

Hypericin-mediated photodynamic therapy induces apoptosis in K562 human leukemia cells through JNK pathway modulation

Contact Info

Product

Resources

About