This survey study was aimed at determining the Rational Drug Use (RDU) behaviors and health perceptions of the staff working in a state university.The population of this cross-sectional study consists of all the staff working in a state university in Turkey. The study sample consisted of a total of 342 staff including academicians, administrative personnel, janitors and security guards. The data were collected through an online questionnaire due to pandemic precautions. The obtained data were recorded on the SPSS version 18 program and descriptive statistics, normality tests, Mann-Whitney U test, Kruskal-Wallis test, and Spearman correlation were used in the analysis.The mean age of the participants in the study were found to be 36,54±8,05. 79,2% of the participants were male. 29,2% of them had a PhD. 44,4% were academic staff working in all departments while 55,6% included administrative personnel, janitors and security guards. The RDU knowledge mean scores of the participants (35,69±5,30) were considered sufficient. It was found that factors such as personal and parental educational status, profession, socioeconomic level, family type, gender, and distance from the health center of the residence had important effects on Rational Drug Use (p<0,05). The participants' health perceptions were at moderate level (51,15±7,97). It was revealed that educational status, profession, and socioeconomic level affected the perception of health (p<0,05). To improve rational drug use and health perceptions, it is crucial to reveal the current situation, and to determine the level of knowledge and attitudes of individuals. Training, studies, and social policies aimed at promoting the perception of health and rational drug use should be made widespread.