Birefringence of muscovite mica

El-Bahrawi, Mohamed; Nagib, N.N.; Khodier, S.A.; Sidki, H. M.

doi:10.1016/s0030-3992(98)00074-7

Cited by 33 publications

(20 citation statements)

References 13 publications

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“…However, mica exhibits a broad absorption spectrum that reduces the observed brightness of the fluorophores (Figure a). In addition, the birefringent properties of mica impart substantial aberration that distorts the PSF . Thus, mica intrinsically reduces the S/N in the fluorescence channel.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the birefringent properties of mica impart substantial aberration that distorts the PSF. 37 Thus, mica intrinsically reduces the S/N in the fluorescence channel. To minimize these effects, while providing sufficient mechanical stability and an appropriate optical working distance, thin slices (<10 μm) of mica are glued to glass coverslips.…”

Section: Resultsmentioning

confidence: 99%

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Orthogonal Probing of Single-Molecule Heterogeneity by Correlative Fluorescence and Force Microscopy

et al. 2018

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Correlative imaging by fluorescence and force microscopy is an emerging technology to acquire orthogonal information at the nanoscale. Whereas atomic force microscopy excels at resolving the envelope structure of nanoscale specimens, fluorescence microscopy can detect specific molecular labels, which enables the unambiguous recognition of molecules in a complex assembly. Whereas correlative imaging at the micrometer scale has been established, it remains challenging to push the technology to the single-molecule level. Here, we used an integrated setup to systematically evaluate the factors that influence the quality of correlative fluorescence and force microscopy. Optimized data processing to ensure accurate drift correction and high localization precision results in image registration accuracies of ∼25 nm on organic fluorophores, which represents a 2-fold improvement over the state of the art in correlative fluorescence and force microscopy. Furthermore, we could extend the Atto532 fluorophore bleaching time ∼2-fold, by chemical modification of the supporting mica surface. In turn, this enables probing the composition of macromolecular complexes by stepwise photobleaching with high confidence. We demonstrate the performance of our method by resolving the stoichiometry of molecular subpopulations in a heterogeneous EcoRV-DNA nucleoprotein ensemble.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Orthogonal Probing of Single-Molecule Heterogeneity by Correlative Fluorescence and Force Microscopy

et al. 2018

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“…The pairs of highly identical wave-plates have been selected for the experiment. The identity of the pair of the wave-plates was checked by the method similar to one described in paper [14]. The deviation in the phase retardance between the selected wave-plates in each pairs did not exceed 0.1 • .…”

Section: Resultsmentioning

confidence: 99%

Properties of an adjustable quarter-wave system under conditions of multiple beam interference

Bibikova

Кундикова

2013

Appl. Opt.

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We investigate the polarimetric properties of an adjustable two plate quarter-wave system. We take into account multiple beam interference within single wave plates. Different adjustments of an adjustable two plate quarter-wave system are required for the production of the left-handed and the right-handed circular polarized coherent light. We investigate experimentally laser light polarization conversion by the systems consisting of two birefringent mica plates. An adjustable two plate quarter-wave system produces high-quality circularly polarized coherent light with the intensity-related ellipticity better than 0.99 at any wavelength.

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“…In contrast, for imaging of the much smaller proteins and protein complexes the most commonly employed AFM substrate is muscovite mica (with an average roughness of 0.05 nm versus approximately 0.5 nm for glass). However, for fluorescence microscopy, mica possesses two unfavorable properties: light absorbance and birefringence [18], causing decreased SNR and distortions of the fluorescence signals, respectively, which lead to reduced center location and ultimately image registration accuracies [19]. We analyzed the effects of signal distortion and SNR on registration accuracy in simulation studies (Supp.…”

Section: Resultsmentioning

confidence: 99%

High accuracy FIONA–AFM hybrid imaging

Fronczek¹,

Quammen²,

Wang³

et al. 2011

Ultramicroscopy

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Multi-protein complexes are ubiquitous and play essential roles in many biological mechanisms. Single molecule imaging techniques such as electron microscopy (EM) and atomic force microscopy (AFM) are powerful methods for characterizing the structural properties of multi-protein and multi-protein–DNA complexes. However, a significant limitation to these techniques is the ability to distinguish different proteins from one another. Here, we combine high resolution fluorescence microscopy and AFM (FIONA–AFM) to allow the identification of different proteins in such complexes. Using quantum dots as fiducial markers in addition to fluorescently labeled proteins, we are able to align fluorescence and AFM information to ≥8 nm accuracy. This accuracy is sufficient to identify individual fluorescently labeled proteins in most multi-protein complexes. We investigate the limitations of localization precision and accuracy in fluorescence and AFM images separately and their effects on the overall registration accuracy of FIONA–AFM hybrid images. This combination of the two orthogonal techniques (FIONA and AFM) opens a wide spectrum of possible applications to the study of protein interactions, because AFM can yield high resolution (5–10 nm) information about the conformational properties of multi-protein complexes and the fluorescence can indicate spatial relationships of the proteins in the complexes.

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Birefringence of muscovite mica

Cited by 33 publications

References 13 publications

Orthogonal Probing of Single-Molecule Heterogeneity by Correlative Fluorescence and Force Microscopy

Orthogonal Probing of Single-Molecule Heterogeneity by Correlative Fluorescence and Force Microscopy

Properties of an adjustable quarter-wave system under conditions of multiple beam interference

High accuracy FIONA–AFM hybrid imaging

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