Birnavirus‐associated proventriculitis in French broiler chickens

Abstract

detection of CPnv, smears of proventriculus from four birds were spotted onto FTa Cards (Whatman) and shipped to the Clinical virology Laboratory, north Carolina state University, Usa. The rT-PCr assays were positive for three of four samples, confirming the molecular detection of CPnv. The sequence of one PCr product showed 99 per cent nucleotide identity with the reference CPnv sequence (genBank accession number hM038436). as far as we are aware, this is the first documented case of CPnvassociated proventriculitis outside the Usa. nevertheless, it is unlikely that this condition has never occurred before, in France or other european countries, and a thorough analysis of archival reports and archived tissues will likely show that proventriculitis caused by CPnv may be anything but exceptional in the field. Poultry veterinarians and laboratory staff should therefore play a key role in determining the prevalence and the pathological significance of CPnv in broiler chickens, on a global scale.

Detection of transmissible viral proventriculitis and chicken proventricular necrosis virus in the UK

“…Cases of chickens with lymphocytic and necrotizing proventriculitis, consistent with TVP, have been reported in the USA (Guy et al, 2011b), South Korea (Kim et al, 2015) and several countries in Europe (Kouwenhoven et al, 1978;Grau-Roma et al, 2010;Marguerie et al, 2011). However, as far as the authors are aware, this is the first peer-reviewed description of TVP as well as of CPNV in the UK.…”

“…TVP was first described in the Netherlands almost 40 years ago (Kouwenhoven et al, 1978). Since then, it has been reported in several countries in North America (Guy et al, 2011b;Noiva et al, 2015), Europe (Grau-Roma et al, 2010;Marguerie et al, 2011), and Asia (Kim et al, 2015). Since the emergence of the disease, there has been considerable discussion as to the aetiological agent responsible.…”

“…A few years ago, a new birnavirus, named chicken proventricular necrosis virus (CPNV), was detected in naturally and experimentally reproduced TVP-affected cases in the USA and proposed to be its cause (Guy et al, 2011a(Guy et al, , 2011b. CPNV has subsequently been detected in a few other studies in TVP-affected broiler chickens in France and the USA (Marguerie et al, 2011;Noiva et al, 2015).…”

“…Although few cases with proventricular lesions compatible to TVP have previously been reported in the UK 1 (Randall & Reece, 1996), as far as the authors are aware, there is no peer-reviewed report indicating the presence of either TVP or CPNV in the UK. Based on the recent description of both TVP (Grau-Roma et al, 2010;Marguerie et al, 2011) and CPNV (Marguerie et al, 2011) in Europe, the aims of this study were to determine whether TVP and CPNV are present in chickens in the UK, as well as to evaluate their association suggested previously.…”

Chicken proventricular necrosis virus related transmissible viral proventriculitis in broiler chickens in Poland

“…The rst reports on TVP date back to 1978 and come from the Netherlands [2], when Kouwenhoven et al reported a case of proventriculitis in commercial chicken broilers and proved that TVP was induced by an infectious factor. Since then, TVP cases have been identi ed and reported in the USA, Australia, China, South Korea, Spain, France, the UK, and Poland among other countries [3][4][5][6][7][8][9][10].…”