Birth after intracytoplasmic sperm injection with use of testicular sperm from men with kartagener/immotile cilia syndrome

Çayan, Selahíttín; Conaghan, J.; Schriock, Eldon D.; Ryan, Isabelle P.; Black, Lauri D.; Turek, Paul J.

doi:10.1016/s0015-0282(01)01974-4

Cited by 69 publications

(46 citation statements)

References 4 publications

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“…The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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Purpose To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture. Materials and methods Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage. Results 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks. Conclusions The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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“…The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

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“…It has been suggested that the probability of injecting viable (but immotile) spermatozoa was greater when testicular sperms were used [17], while ejaculated sperm, even if tested viable, were less successful [16]. Indeed, several pregnancies were achieved using ICSI with TESE material [5,17,18].…”

Section: Discussionmentioning

confidence: 99%

“…Even using the activation methods discussed so far may not keep embryologists from facing complete fertilization failure [13,16,26,27], fertilization problems [17,18] or nonappearance of pregnancy [6,7]. Possible explanations for this unwanted happening are rare but could be related to the quality of the selected spermatozoon.…”

Section: Discussionmentioning

confidence: 99%

“…Neither continuous reduction of period of abstinence (from 3 to 1 day) nor application of a ready-to-use theophylline (SpermMobil, Gynemed, Lensahn, Germany) resulted in motile spermatozoa. Consequently, electron microscope analysis of spermatozoa was recommended in order to quantify the percentage of sperms with normal 9+2 microtubule structure [17] which the patient refused because the procedure appeared too time-consuming. For the same reasons testicular biopsy was not accepted; much rather, the couple decided to opt for a cycle of controlled ovarian hyperstimulation (COH) with the husband's immotile sperms from the ejaculate.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous approaches have been published suggesting hypoosmotic swell (HOS) test [11,16], usage of testicular sperm [5,17,18] or chemical activation of immotile sperm [12,14] in order to increase the percentage of viable sperm available for ICSI. This is the first report of a healthy twin live-birth after usage of ionophore treatment in a patient suffering from Kartagener syndrome who showed failed sperm activation with the dimethylxanthine theophylline.…”

Section: Introductionmentioning

confidence: 99%

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Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Ebner

Maurer

Oppelt

et al. 2015

J Assist Reprod Genet

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Purpose To evaluate whether it is a feasible option to target the oocyte (with Ca 2+ -ionophore) in case that sperm motility cannot be restored in Kartagener syndrome. Methods A case of a male Kartagener syndrome with exclusively immotile spermatozoa that did not react to the dimethylxanthine theophylline. Thus, half of the associated oocytes were treated for 15 min with the ready-to-use-ionophore CultActive immediately after ICSI whereas the other 50 % were injected with routine ICSI without artificial oocyte activation. Rates of fertilization, blastulation, pregnancy and live birth were evaluated. Results Fertilization check revealed that none of the conventionally injected but 4/6 (66.7 %) of the artificially activated oocytes showed two pronuclei. Three embryos were of good and one of fair quality. Corresponding blastocyst formation rate was 3 out of 4 (75 %). A double embryo transfer led to a healthy twin birth in the 34th week of gestation (two boys with a birth weight of 1724 g and 2199 g). Conclusions This case indicates that Ca 2+-ionophore treatment in cycles from theophylline-resistant Kartagener syndrome patients is a feasible option. The future will show if routine application of A23187 in Kartagener or primary cilia dyskinesis patients will be of benefit.

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Poor Quality Ejaculate Sperm: Do the Data Support the Use of Testis Sperm?

Oates

2013

Biennial Review of Infertility

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Birth after intracytoplasmic sperm injection with use of testicular sperm from men with kartagener/immotile cilia syndrome

Cited by 69 publications

References 4 publications

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

Poor Quality Ejaculate Sperm: Do the Data Support the Use of Testis Sperm?

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