Birth defects and anti–heat shock protein 70 antibodies in early pregnancy

Child, D. F.; Hudson, Peter; Hunter-Lavin, Claire; Mukhergee, Sagarika; China, Susnata; Williams, Clare; Williams, John

doi:10.1379/csc-130r1.1

Cited by 21 publications

(14 citation statements)

References 25 publications

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“…Child et al (2006) reported that serum anti-hsp70 antibody levels are significantly elevated at 16 weeks of gestation in women who later gave birth to babies with birth defects (cleft lip or palate or neural tube defects). A recent study identified altered production of heat shock proteins in development of chemical-induced cleft palate formation in mice (Zhu et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Saito

Damasceno

Dallaqua

et al. 2013

Cell Stress and Chaperones

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We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; nontreated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R0 0.5908, P00.0024; R00.4877, P00.0134, respectively) and the number of malformations per fetus (R 00.6103, P0 0.0015; R 00.4875, P 00.0134, respectively). The antihsp60 IgG concentration was correlated with the number of malformations per fetus (R00.3887, P00.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R00.3999, P00.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.

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Section: Discussionmentioning

confidence: 99%

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Saito

Damasceno

Dallaqua

et al. 2013

Cell Stress and Chaperones

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“…Furthermore, the presence of anti-Hsp60 and anti-Hsp70 antibodies in the serum and formation of Hsp60-and Hsp70-immune complexes in the placenta were associated with preterm birth (Ziegert et al 1999). Recently, serum anti-Hsp70 Data are presented as median (25-75 percentile) Hsp heat shock protein, AU arbitrary unit, IUGR intrauterine growth restriction levels were found to be significantly elevated at 16 weeks of gestation in women who later gave birth to babies with birth defects, suggesting a prior increase in Hsp70 expression (Child et al 2006). Infection/inflammation and oxidative and hemodynamic stress have also been implicated in the pathogenesis of preeclampsia (von Dadelszen et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Circulating anti-heat-shock-protein antibodies in normal pregnancy and preeclampsia

Molvarec

Derzsy

Kocsis

et al. 2009

Cell Stress and Chaperones

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It has been previously reported that circulating anti-heat-shock-protein (Hsp) antibody levels are elevated in cardiovascular disorders. The aim of the present study was to determine circulating antihuman Hsp60, antimycobacterial Hsp65, and antihuman Hsp70 antibody levels in healthy pregnant women and preeclamptic patients and to investigate their relationship to the clinical characteristics of the study subjects, as well as to the markers of inflammation (C-reactive protein (CRP)), endothelial activation (von Willebrand factor antigen), or endothelial injury (fibronectin), oxidative stress (malondialdehyde) and to serum Hsp70 levels. Ninety-three preeclamptic patients and 127 normotensive healthy pregnant women were involved in this case control study. Serum anti-Hsp60, anti-Hsp65, anti-Hsp70, and Hsp70 levels were measured with enzyme-linked immunosorbent assay (ELISA). Serum CRP levels were determined by an autoanalyzer using the manufacturer's kit. Plasma von Willebrand factor antigen levels were quantified by ELISA, while plasma fibronectin concentration by nephelometry. Plasma malondialdehyde levels were measured by the thiobarbituric-acid-based colorimetric assay. For statistical analyses, nonparametric methods were applied. Anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibodies were detected in all of our serum samples. There were no significant differences in serum anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibody levels between the control and preeclamptic groups. Serum levels of Hsp70 and CRP, as well as plasma levels of VWF antigen, fibronectin, and malondialdehyde, were significantly higher in preeclamptic patients than in normotensive healthy pregnant women. Serum anti-Hsp60 antibody levels showed significant correlations with serum anti-Hsp65 antibody levels both in the control and the preeclamptic groups (Spearman R = 0.55 and 0.59; p < 0.001, respectively). However, no other relationship was found between clinical features (maternal age, smoking status, parity, body mass index, gestational age at blood draw, systolic and diastolic blood pressure, gestational age at delivery, and fetal birth weight) and measured laboratory parameters of the study subjects and serum anti-Hsp antibody levels in either study group. In conclusion, anti-Hsp60 and anti-Hsp70 antibodies as naturally occurring autoantibodies are present in the peripheral circulation of healthy pregnant women. Nevertheless, humoral immunity against heat shock proteins was not associated with preeclampsia. Further studies are warranted to explore the role of heat shock proteins and immune reactivity to them in the immunobiology of normal pregnancy and preeclampsia.

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“…22 High level of antibodies to Hsp70 in early pregnancy correlated with increased birth defects. 23 There are numerous reports on Hsp70 expression on the surface of cancer cells. Hsp70 surface expression may be regarded as another feature shared by ESC and cancer cells.…”

Section: Cellsmentioning

confidence: 99%

Heat shock induces apoptosis in human embryonic stem cells but a premature senescence phenotype in their differentiated progeny

Алексеенко

Zemelko²,

Зенин³

et al. 2012

Cell Cycle

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Birth defects and anti–heat shock protein 70 antibodies in early pregnancy

Cited by 21 publications

References 25 publications

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

Circulating anti-heat-shock-protein antibodies in normal pregnancy and preeclampsia

Heat shock induces apoptosis in human embryonic stem cells but a premature senescence phenotype in their differentiated progeny

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