Birth of healthy twins after fertilization with in vitro cultured spermatids from a patient with massive in vivo apoptosis of postmeiotic germ cells

Cruz-Navarro, Natalio; Moreno, Eduardo; Cañete, Maria Teresa A.; Mendoza, Carmen

doi:10.1016/s0015-0282(00)01569-7

Cited by 25 publications

(11 citation statements)

References 4 publications

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“…The reported low fertilization rate associated with spermatid injection may be related to a high frequency of apoptosis among postmeiotic germ cells. In vitro culture of testicular tissue may improve the results (Tesarik et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Mansour

Fahmy

Taha

et al. 2003

Journal of Andrology

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Almost one-third of all patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) have cancelled cycles due to failure to find spermatozoa. For these patients, every attempt should be made to rescue the cycles by searching for spermatids. In this retrospective study, we report our experience in using elongating (stage Sb2) and elongated (stage Sc and Sd1) spermatids for ICSI. The study included 488 consecutive ICSI and TESE cycles performed for 452 patients with nonobstructive azoospermia. In 179 (36.7%) cycles, neither spermatozoa nor mature spermatids (stage Sd2) suitable for injection were found. After an extensive search only Sb2, Sc, and Sd1 spermatids were found in 22 of these 179 cycles (12.3%). These spermatids were used for injection of retrieved oocytes. The fertilization rate was 33.2%, and 19 patients (86.4%) reached the embryo transfer stage. In 6 cycles a chemical pregnancy occurred, and 3 clinical pregnancies were established, resulting in the delivery of 3 healthy boys with normal karyotypes. When normal living spermatozoa or mature spermatids (stage Sd2) cannot be found during TESE, late spermatids (stage Sb2, Sc, and Sd1) can be used successfully and result in the delivery of healthy offspring.

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Section: Discussionmentioning

confidence: 99%

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Mansour

Fahmy

Taha

et al. 2003

Journal of Andrology

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“…In addition, culture of human round spermatids on Vero cell monolayer were induced to elongated spermatids and even to mature spermatozoa with normal morphology, which were even used to fertilize human oocyte and to develop embryo (Balaban et al 1999;Cremades et al 1999Cremades et al , 2001Tesarik et al 2000c;Tanaka et al 2003). These results may suggest that Vero cells or their products, under certain conditions, could be involved in the induction of meiosis and spermiogenesis.…”

Section: Induction Of Meiosis In Vitromentioning

confidence: 98%

In vitroculture of testicular germ cells: Regulatory factors and limitations

2007

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Spermatogenesis is regulated mainly by endocrine factors and also by testicular paracrine/autocrine growth factors. These factors are produced by Sertoli cells, germ cells, peritubular cells and interstitial cells, mainly Leydig cells and macrophages. The interactions and the ratio between Sertoli and germ cells in the seminiferous tubules ensure successful spermatogenesis. In order to culture spermatogonial stem cells (SSCs) in vitro, researchers tried to overcome some of the obstacles -- such as the low number of stem cells in the testis, absence of specific markers to identify SSCs -- in addition to difficulties in keeping the SSCs alive in culture. Recently, some growth factors important for the proliferation and differentiation of SSCs were identified, such as glial cell line derived neurotrophic factor (GDNF), stem cell factor (SCF) and leukemia inhibitory factor (LIF); also, markers for SSCs at different stages were reported. Therefore, some groups succeeded in culturing SSCs (under limitations), or more differentiated cells and even were able to produce in vitro germ cells from embryonic stem cells. Thus, success in culturing SSCs is dependent on understanding the molecular mechanisms behind self-renewal and differentiation. Culture of SSCs should be a good tool for discovering new therapeutic avenue for some infertile men or for patients undergoing chemotherapy/radiotherapy (pre-puberty or post-puberty).

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“…Later, the birth of two healthy babies (twins) was achieved by the same group using an in vitro culture system that supported the postmeiotic differentiation of round spermatids from patients with incomplete arrest of spermiogenesis and a high frequency of apoptosis among postmeiotic germ cells (48). …”

Section: In Vitro Maturation and Artmentioning

confidence: 99%

Intracytoplasmic spermatid injection and in vitro maturation: fact or fiction?

Vloeberghs¹,

Verheyen²,

Tournaye³

2013

Clinics

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Intracytoplasmic injection with testicular spermatozoa has become a routine treatment in fertility clinics. Spermatozoa can be recovered in half of patients with nonobstructive azoospermia. The use of immature germ cells for intracytoplasmic injection has been proposed for cases in which no spermatozoa can be retrieved. However, there are low pregnancy rates following intracytoplasmic injection using round spermatids from men with no elongated spermatids or spermatozoa in their testes. The in vitro culture of immature germ cells to more mature stages has been proposed as a means to improve this poor outcome. Several years after the introduction of intracytoplasmic injection with elongating and round spermatids, uncertainty remains as to whether this approach can be considered a safe treatment option. This review outlines the clinical and scientific data regarding intracytoplasmic injection using immature germ cells and in vitro matured germ cells.

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Birth of healthy twins after fertilization with in vitro cultured spermatids from a patient with massive in vivo apoptosis of postmeiotic germ cells

Cited by 25 publications

References 4 publications

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

In vitroculture of testicular germ cells: Regulatory factors and limitations

Intracytoplasmic spermatid injection and in vitro maturation: fact or fiction?

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