Birth of Lambs after Direct Transfer of Vitrified Ovine Embryos.

Abstract

Abstract. The aim of this study was to investigate in vivo viability of vitrified ovine embryos after direct transfer using a laparoscope. In vivo-derived ovine morulae and blastocysts were equilibrated in 25% glycerol and 25% ethylene glycol solution, loaded into a heat-pulled straw and vitrified. Thirty-eight embryos were transferred into 19 recipients (2 embryos per recipient) and pregnancy was examined at 54-59 days after transfer. The pregnancy, implantation, lambing, and survival rates were 15.8% (3/19), 10.5% (4/38), 15.8% (3/19) and 7.9% (3/38), respectively. The result indicates that viable lambs can be produced by direct transfer of vitrified ovine embryos.

Viability of OPS Vitrified Sheep Embryos After Direct Transfer

“…However, our lower results could not be applied to human error because this experiment was performed by the same technical team, suggesting that the observed difference may be related to the interval between flushing and embryos transference (10-12 h) or to the different batches and breed of female recipients It is desirable that cryopreserved embryos are transferred into the uterus as soon as possible, and the interval between post-thawing and transference has a great influence on subsequent viability. In this experiment, the interval between flushing and cryopreservation was about 8 h. In addition to that, the stepwise for warming embryos requires a long time until the transference and the number of manipulations affect embryonic survival (Okada et al 2002). Dattena et al (2004) reported no difference about embryo viability if one or three stepwise warming dilution was employed.…”

“…It is desirable that cryopreserved embryos are transferred into the uterus as soon as possible, and the interval between post‐thawing and transference has a great influence on subsequent viability. In this experiment, the interval between flushing and cryopreservation was about 8 h. In addition to that, the stepwise for warming embryos requires a long time until the transference and the number of manipulations affect embryonic survival (Okada et al. 2002).…”